Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   U6037_RS24690 Genome accession   NZ_CP140706
Coordinates   5595457..5595894 (-) Length   145 a.a.
NCBI ID   WP_322844841.1    Uniprot ID   -
Organism   Pseudomonas sp. B33.4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5590457..5600894
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U6037_RS24670 - 5591290..5594241 (+) 2952 WP_322844839.1 DUF748 domain-containing protein -
  U6037_RS24675 - 5594241..5594537 (+) 297 WP_322844840.1 DUF2845 domain-containing protein -
  U6037_RS24685 - 5594807..5595160 (-) 354 WP_150650535.1 BON domain-containing protein -
  U6037_RS24690 pilA/pilAI 5595457..5595894 (-) 438 WP_322844841.1 pilin Machinery gene
  U6037_RS24695 pilB 5596120..5597820 (+) 1701 WP_322844842.1 type IV-A pilus assembly ATPase PilB Machinery gene
  U6037_RS24700 pilC 5597823..5599040 (+) 1218 WP_322844843.1 type II secretion system F family protein Machinery gene
  U6037_RS24705 pilD 5599042..5599914 (+) 873 WP_322844844.1 A24 family peptidase Machinery gene
  U6037_RS24710 coaE 5599911..5600534 (+) 624 WP_322844845.1 dephospho-CoA kinase -
  U6037_RS24715 yacG 5600531..5600731 (+) 201 WP_007959585.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14572.67 Da        Isoelectric Point: 8.4936

>NTDB_id=915042 U6037_RS24690 WP_322844841.1 5595457..5595894(-) (pilA/pilAI) [Pseudomonas sp. B33.4]
MKKQQGFTLIELLIVVAIIGILATIALPQYSKYQARSKVTAGLAEISAMKVPFEDTINQGTAPDITTVNGGTTTTSNCTV
AVTGTAATGAGSISCTLLNAPGPVLGKTITLTRSGAATGNTSGLWTCGTTVNADYSPKGCTASGT

Nucleotide


Download         Length: 438 bp        

>NTDB_id=915042 U6037_RS24690 WP_322844841.1 5595457..5595894(-) (pilA/pilAI) [Pseudomonas sp. B33.4]
ATGAAAAAACAACAAGGTTTTACTCTGATCGAGCTGTTGATCGTGGTGGCGATCATCGGGATTCTGGCGACCATCGCCCT
GCCGCAGTATTCCAAGTATCAGGCTCGGTCGAAGGTGACGGCTGGGCTGGCGGAAATCAGCGCGATGAAGGTGCCGTTCG
AGGACACCATCAACCAAGGGACCGCTCCGGATATAACCACTGTAAATGGCGGTACAACCACCACCTCCAACTGTACGGTG
GCGGTTACAGGCACAGCGGCGACGGGTGCGGGCAGCATCAGTTGCACGTTGCTCAACGCTCCGGGACCGGTACTGGGCAA
AACCATCACGCTCACTCGTTCGGGCGCTGCTACCGGCAATACCTCTGGCTTGTGGACCTGTGGCACTACTGTTAACGCGG
ATTATTCGCCAAAAGGCTGTACCGCCAGCGGTACGTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

48.611

99.31

0.483
  pilA Acinetobacter baumannii strain A118

46.897

100

0.469
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.857

100

0.455
  pilA Vibrio cholerae strain A1552

42.857

100

0.455
  pilA Vibrio cholerae C6706

42.857

100

0.455
  pilA Pseudomonas aeruginosa PAK

40.909

100

0.434
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.972

97.241

0.428
  comP Acinetobacter baylyi ADP1

39.869

100

0.421
  pilA Vibrio parahaemolyticus RIMD 2210633

41.135

97.241

0.4
  pilA2 Legionella pneumophila str. Paris

40

96.552

0.386
  pilA2 Legionella pneumophila strain ERS1305867

39.286

96.552

0.379
  pilA Ralstonia pseudosolanacearum GMI1000

39.259

93.103

0.366