Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   U6037_RS24695 Genome accession   NZ_CP140706
Coordinates   5596120..5597820 (+) Length   566 a.a.
NCBI ID   WP_322844842.1    Uniprot ID   -
Organism   Pseudomonas sp. B33.4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5591120..5602820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U6037_RS24670 - 5591290..5594241 (+) 2952 WP_322844839.1 DUF748 domain-containing protein -
  U6037_RS24675 - 5594241..5594537 (+) 297 WP_322844840.1 DUF2845 domain-containing protein -
  U6037_RS24685 - 5594807..5595160 (-) 354 WP_150650535.1 BON domain-containing protein -
  U6037_RS24690 pilA/pilAI 5595457..5595894 (-) 438 WP_322844841.1 pilin Machinery gene
  U6037_RS24695 pilB 5596120..5597820 (+) 1701 WP_322844842.1 type IV-A pilus assembly ATPase PilB Machinery gene
  U6037_RS24700 pilC 5597823..5599040 (+) 1218 WP_322844843.1 type II secretion system F family protein Machinery gene
  U6037_RS24705 pilD 5599042..5599914 (+) 873 WP_322844844.1 A24 family peptidase Machinery gene
  U6037_RS24710 coaE 5599911..5600534 (+) 624 WP_322844845.1 dephospho-CoA kinase -
  U6037_RS24715 yacG 5600531..5600731 (+) 201 WP_007959585.1 DNA gyrase inhibitor YacG -
  U6037_RS24720 - 5600738..5600953 (-) 216 WP_008078491.1 hypothetical protein -
  U6037_RS24725 - 5601019..5601708 (-) 690 WP_322844846.1 energy-coupling factor ABC transporter permease -
  U6037_RS24730 - 5601827..5602453 (+) 627 WP_008078503.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62588.52 Da        Isoelectric Point: 5.9184

>NTDB_id=915043 U6037_RS24695 WP_322844842.1 5596120..5597820(+) (pilB) [Pseudomonas sp. B33.4]
MNDIALSGLAKQLVQAELLTEKSAQQASQQAQRNRLSLVSYLVQNKLVKSSQVAEIASEHFGMALLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHTSGLEDMADVDLDG
VDVESIDDNKQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCGHCKKPIEIPRETLIKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDLQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=915043 U6037_RS24695 WP_322844842.1 5596120..5597820(+) (pilB) [Pseudomonas sp. B33.4]
ATGAATGACATCGCTTTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCCGAACTGCTCACGGAAAAAAGCGCCCAGCAAGC
CTCGCAACAGGCCCAGCGTAATCGCTTGTCGCTGGTCAGCTATCTGGTGCAGAACAAACTGGTCAAGAGCTCACAGGTCG
CCGAAATCGCCTCCGAGCATTTCGGCATGGCCTTGCTTGATCTCAATTGCCTCGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGCGAAAAACTGGTCCGCCAGCATCACGCCCTGCCCTTGTGGCGGCGAGGCAACAAGCTGTTCGTGGGGATTTCCGA
TCCGAGCAATCATCAAGCGATCAACGACATTCAGTTCAGCACCGGACTGAGCACCGAAGCCATCCTGGTCGAGGACGACA
AGCTCACCGATGCCATCGAGAAGTTCTTCGACACCCACACCAGTGGCCTGGAGGACATGGCCGATGTCGATCTCGACGGG
GTCGATGTCGAGTCCATCGACGACAACAAGCAAGACGCTATCGGCGGACTCGACGCCGACGATGCGCCGGTGGTGCGTTT
CGTTCACAAGATGTTGCTGGATGCGATCAAGAGTGGCTCTTCCGACTTGCACTTCGAACCCTACGAGAAGAACTACCGGG
TACGGGTGCGCACCGACGGCATTCTGCGCGAAGTGGCCAAGCCACCGATTCAACTGGCCGGGCGCATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTCGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAAATGCGCCTGTCGAAGAGCAA
GTCGATCGATTTTCGGGTCAACACGCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGCG
CACAGATCGGCATCGACGCCCTCGGCTATGAGCCCGAGCAAAAGGATCTGTACATGGCCGCTCTCAAGCAGCCACAGGGC
ATGATTCTGGTCACCGGGCCCACGGGCTCGGGGAAAACCGTGTCGCTGTACACCGGCCTGAATATCCTTAACACCGTCGA
CATCAATATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAGACAAG
GTCTCGACTTTGCTCAGGCACTGCGCTCGTTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACCTCGTGCTCTCGACCCTGCACACCAACAGCGCGGCGGA
AACCCTGACCCGCCTGCACAACATGGGCATTCCCGGTTTCAACATCGCCACATCGGTCAGCCTGATTATCGCTCAGCGTC
TGGCGCGCAAGCTCTGCGGCCATTGCAAGAAACCCATCGAGATCCCCCGCGAAACCTTGATCAAGGAAGGTTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAACGGCGGCTACAAAGGACGCGTGGGGAT
ATATGAAGTGGTGAAGAACACACCAGAGCTGCAACGTTTGATCATGGCCGAAGGCAACTCGCTGGAAATCGACCTGCAGA
TGCGTCGCGATGGCTTCGACGACCTGCGCACCTCAGGGCTGCACAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AATCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.615

99.47

0.583
  pilB Acinetobacter baumannii D1279779

57.979

99.647

0.578
  pilB Legionella pneumophila strain ERS1305867

56.007

100

0.56
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

50

99.293

0.496
  pilB Vibrio campbellii strain DS40M4

48.862

100

0.493
  pilF Neisseria gonorrhoeae MS11

49.378

99.47

0.491
  pilF Thermus thermophilus HB27

36.918

98.587

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.679

93.64

0.362