Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   U4W77_RS18645 Genome accession   NZ_CP140493
Coordinates   3846535..3847515 (-) Length   326 a.a.
NCBI ID   WP_022651830.1    Uniprot ID   A0AAE8X1F8
Organism   Enterobacter hormaechei strain 2023CK-01313     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3841535..3852515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U4W77_RS18615 (U4W77_18565) - 3842413..3842910 (+) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -
  U4W77_RS18620 (U4W77_18570) endA 3843005..3843712 (+) 708 WP_022651828.1 deoxyribonuclease I -
  U4W77_RS18625 (U4W77_18575) rsmE 3843764..3844495 (+) 732 WP_022651829.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  U4W77_RS18630 (U4W77_18580) gshB 3844527..3845474 (+) 948 WP_003860032.1 glutathione synthase -
  U4W77_RS18635 (U4W77_18585) - 3845549..3846109 (+) 561 WP_003860031.1 YqgE/AlgH family protein -
  U4W77_RS18640 (U4W77_18590) ruvX 3846109..3846525 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  U4W77_RS18645 (U4W77_18595) pilT 3846535..3847515 (-) 981 WP_022651830.1 type IV pilus twitching motility protein PilT Machinery gene
  U4W77_RS18650 (U4W77_18600) - 3847533..3848234 (+) 702 WP_003860026.1 YggS family pyridoxal phosphate-dependent enzyme -
  U4W77_RS18655 (U4W77_18605) - 3848256..3848822 (+) 567 WP_003860024.1 YggT family protein -
  U4W77_RS18660 (U4W77_18610) yggU 3848819..3849115 (+) 297 WP_003860023.1 DUF167 family protein YggU -
  U4W77_RS18665 (U4W77_18615) - 3849119..3849712 (+) 594 WP_022651831.1 XTP/dITP diphosphatase -
  U4W77_RS18670 (U4W77_18620) hemW 3849705..3850853 (+) 1149 WP_003860019.1 radical SAM family heme chaperone HemW -
  U4W77_RS18675 (U4W77_18625) - 3850910..3851272 (+) 363 WP_022651832.1 endonuclease domain-containing protein -
  U4W77_RS18680 (U4W77_18630) - 3851385..3852101 (-) 717 WP_003862419.1 DUF2884 domain-containing protein -
  U4W77_RS18685 (U4W77_18635) - 3852159..3852485 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35653.57 Da        Isoelectric Point: 6.2335

>NTDB_id=914539 U4W77_RS18645 WP_022651830.1 3846535..3847515(-) (pilT) [Enterobacter hormaechei strain 2023CK-01313]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFSEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=914539 U4W77_RS18645 WP_022651830.1 3846535..3847515(-) (pilT) [Enterobacter hormaechei strain 2023CK-01313]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAATG
ACGAACAGCAGGGCGCCTGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTGCGCCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACTCTGGCCGCGATGGTCGATTTTCTCAATCACCAGACAGACGGGCACATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTTCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGTGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGTGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAATCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377