Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PsasTeo6_RS25010 Genome accession   NZ_CP139776
Coordinates   5477639..5478637 (-) Length   332 a.a.
NCBI ID   WP_322265040.1    Uniprot ID   -
Organism   Pseudomonas asiatica strain Teo6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5472639..5483637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PsasTeo6_RS24990 (PsasTeo6_40040) argS 5473816..5475552 (+) 1737 WP_013974613.1 arginine--tRNA ligase -
  PsasTeo6_RS24995 (PsasTeo6_40048) - 5475554..5476252 (+) 699 WP_003257831.1 SPOR domain-containing protein -
  PsasTeo6_RS25000 (PsasTeo6_40056) - 5476290..5476709 (-) 420 WP_013974614.1 TM2 domain-containing protein -
  PsasTeo6_RS25005 (PsasTeo6_40064) - 5476876..5477511 (+) 636 WP_322265039.1 C40 family peptidase -
  PsasTeo6_RS25010 (PsasTeo6_40072) pilT 5477639..5478637 (-) 999 WP_322265040.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PsasTeo6_RS25015 (PsasTeo6_40080) - 5478703..5479389 (+) 687 WP_061550317.1 YggS family pyridoxal phosphate-dependent enzyme -
  PsasTeo6_RS25020 (PsasTeo6_40088) proC 5479428..5480246 (+) 819 WP_182328848.1 pyrroline-5-carboxylate reductase -
  PsasTeo6_RS25025 (PsasTeo6_40096) - 5480257..5480847 (+) 591 WP_013974619.1 YggT family protein -
  PsasTeo6_RS25030 (PsasTeo6_40104) - 5480947..5482086 (+) 1140 WP_013974620.1 homoserine O-acetyltransferase -
  PsasTeo6_RS25035 (PsasTeo6_40112) metW 5482094..5482714 (+) 621 WP_039612534.1 methionine biosynthesis protein MetW -
  PsasTeo6_RS25040 (PsasTeo6_40120) - 5482737..5483171 (+) 435 WP_003257823.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35841.50 Da        Isoelectric Point: 6.3081

>NTDB_id=910917 PsasTeo6_RS25010 WP_322265040.1 5477639..5478637(-) (pilT) [Pseudomonas asiatica strain Teo6]
MDVTDLLARAVDAGASDLHLAAGQLPMLRLDGELQRMALPTLGPVALIEGMAPMLDEDQRRQWAQGDELDLALELPALGR
FRLNLFRQLHGPAATFRLIPGHIATLDELDLGDVFQAVAQCRDGLILVGGPTGSGKSSTLAALLDQLNRDRALHIITLED
PVEVIHSSQRSLVNQREVGRHCGGFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHTRSAVSSVDRL
VEVFATEEKPLVRAMLAESLRLVVAQVLVRRAGGGRVAAREVLVVTPAVRNLVREGRMAQLCSVMQAGGADGMRTMEGAM
RGLKERGLISDL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=910917 PsasTeo6_RS25010 WP_322265040.1 5477639..5478637(-) (pilT) [Pseudomonas asiatica strain Teo6]
ATGGATGTGACCGACCTGTTGGCCCGGGCCGTGGATGCGGGGGCTTCCGACCTGCACCTGGCGGCGGGCCAGTTACCGAT
GTTGCGCCTGGATGGCGAGCTGCAGCGCATGGCCCTGCCGACCTTGGGCCCGGTCGCCTTGATCGAGGGCATGGCACCCA
TGCTGGATGAAGACCAGCGCCGACAGTGGGCCCAGGGTGATGAGCTGGACCTGGCGCTGGAATTGCCGGCGCTAGGGCGC
TTTCGGCTGAATCTGTTTCGCCAGTTGCACGGCCCTGCGGCCACCTTTCGCCTGATCCCGGGGCATATCGCCACGCTCGA
TGAACTCGACCTGGGCGATGTGTTTCAAGCTGTTGCGCAGTGCCGAGATGGCTTGATCCTCGTCGGTGGGCCGACCGGCA
GTGGCAAGTCCAGCACCTTGGCGGCACTGCTCGACCAGCTGAACCGCGATCGTGCTCTGCATATCATCACCCTTGAGGAC
CCTGTCGAAGTTATCCACAGCAGCCAGCGCAGCCTGGTCAACCAGCGTGAGGTCGGCCGCCATTGCGGTGGGTTCGCCCA
GGGGCTGCGCAGTGCCCTGCGCCAGGACCCGGATGTGATCATGATCGGTGAGCTGCGCGACCTGGAAACCATTCGCCTGG
CCTTGCGTGCGGCTGAGACCGGGCACCTGGTACTGGCGACCGTGCATACGCGTTCGGCGGTGAGCAGTGTTGACCGGTTG
GTGGAGGTGTTCGCTACCGAAGAGAAGCCGTTGGTGCGGGCGATGCTGGCCGAGTCGTTGCGCCTGGTGGTAGCGCAGGT
GCTGGTCAGGCGCGCGGGCGGAGGGCGGGTTGCTGCGCGGGAAGTGCTAGTGGTGACGCCAGCGGTGCGTAACCTGGTTC
GGGAAGGGCGGATGGCGCAGTTGTGTTCGGTGATGCAGGCGGGTGGTGCGGACGGGATGCGGACGATGGAGGGGGCGATG
CGAGGGTTGAAGGAGAGGGGGCTGATCAGCGATCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.121

99.398

0.518
  pilT Acinetobacter nosocomialis M2

51.212

99.398

0.509
  pilT Acinetobacter baumannii D1279779

51.212

99.398

0.509
  pilT Acinetobacter baumannii strain A118

51.212

99.398

0.509
  pilT Pseudomonas stutzeri DSM 10701

50.909

99.398

0.506
  pilT Acinetobacter baylyi ADP1

50.606

99.398

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.544

99.096

0.491
  pilT Vibrio cholerae strain A1552

49.544

99.096

0.491
  pilT Legionella pneumophila strain Lp02

48.788

99.398

0.485
  pilT Legionella pneumophila strain ERS1305867

48.788

99.398

0.485
  pilT Neisseria meningitidis 8013

48.64

99.699

0.485
  pilT Neisseria gonorrhoeae MS11

48.338

99.699

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.921

99.699

0.458
  pilU Pseudomonas stutzeri DSM 10701

37.16

99.699

0.37
  pilU Vibrio cholerae strain A1552

37.117

98.193

0.364