Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   UM396_RS13295 Genome accession   NZ_CP139723
Coordinates   2582136..2583191 (-) Length   351 a.a.
NCBI ID   WP_322310332.1    Uniprot ID   -
Organism   Geobacillus subterraneus strain OXR-76     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2577136..2588191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UM396_RS13265 (UM396_13265) - 2577432..2578100 (-) 669 WP_322310326.1 pilus assembly protein PilO -
  UM396_RS13270 (UM396_13270) - 2578097..2578672 (-) 576 WP_322310327.1 fimbrial protein -
  UM396_RS13275 (UM396_13275) pilM 2578662..2579591 (-) 930 WP_322310328.1 pilus assembly protein PilM -
  UM396_RS13280 (UM396_13280) - 2579617..2580378 (-) 762 WP_322310329.1 A24 family peptidase -
  UM396_RS13285 (UM396_13285) - 2580407..2580850 (-) 444 WP_322310330.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  UM396_RS13290 (UM396_13290) - 2580938..2582149 (-) 1212 WP_322310331.1 type II secretion system F family protein -
  UM396_RS13295 (UM396_13295) pilT 2582136..2583191 (-) 1056 WP_322310332.1 type IV pilus twitching motility protein PilT Machinery gene
  UM396_RS13300 (UM396_13300) - 2583204..2584868 (-) 1665 WP_168368848.1 GspE/PulE family protein -
  UM396_RS13305 (UM396_13305) - 2584865..2586223 (-) 1359 WP_322310333.1 G5 domain-containing protein -
  UM396_RS13310 (UM396_13310) - 2586240..2587019 (-) 780 WP_322310334.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38538.75 Da        Isoelectric Point: 8.0025

>NTDB_id=910732 UM396_RS13295 WP_322310332.1 2582136..2583191(-) (pilT) [Geobacillus subterraneus strain OXR-76]
MKEKLEALLRAAFECRASDVHLTVGAPPIFRLNGELKAYGHERLRLEETEQMAKAIIPPPLWQQFQEAGELDFSYGIAGV
SRFRFNVFKQRSCVSLAVRLIPMRIPTLEELGMPSVLKRIAEKPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMSKHIITL
EDPIEYLHKHGKSIIDQREIGFDTGSFAAGLRAALRQDPDVILVGEMRDLETIQTAVTAAETGHLVLGTLHTASAPAAIE
RMIDVFPPAQQAQIRLQLASVLVAIIAQRLFPNGRKSGRTAALEILINNAAVANLIRNKKEHQIVNIMQTSRNAGMQTME
MSIKELVADGRIDPLAAETYLAGERGEHGAI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=910732 UM396_RS13295 WP_322310332.1 2582136..2583191(-) (pilT) [Geobacillus subterraneus strain OXR-76]
ATGAAGGAAAAGCTTGAGGCGTTATTGCGCGCCGCCTTTGAATGCCGCGCGTCCGATGTGCATTTAACCGTCGGCGCGCC
GCCGATTTTCCGCTTGAATGGGGAGCTGAAGGCATACGGCCATGAGCGGCTTCGTTTGGAAGAGACCGAACAAATGGCAA
AAGCCATCATTCCGCCGCCGCTTTGGCAGCAGTTCCAAGAAGCGGGGGAGCTCGATTTTTCGTACGGCATAGCCGGCGTC
TCGCGCTTCCGTTTCAATGTGTTTAAACAGCGGTCGTGCGTGTCGCTCGCTGTGCGCCTCATTCCGATGCGCATTCCGAC
GCTTGAGGAGCTCGGCATGCCGAGCGTCCTGAAACGCATCGCTGAAAAGCCGCACGGGCTCGTGCTTGTCACCGGGCCGA
CCGGCAGCGGCAAATCGACGACATTGGCGGCGATGATCGACTATATGAACAAAACGATGTCGAAACATATCATTACGCTC
GAGGATCCGATCGAATATTTGCATAAACACGGAAAATCGATCATCGACCAGCGTGAGATCGGATTTGACACCGGCAGTTT
TGCTGCTGGTTTGCGCGCTGCGCTCCGCCAAGATCCGGATGTCATTTTAGTCGGGGAAATGCGCGATTTGGAGACGATTC
AAACGGCGGTCACCGCCGCGGAAACGGGGCATTTAGTGTTAGGCACGCTTCATACAGCGAGCGCGCCGGCGGCAATCGAG
CGGATGATTGACGTCTTTCCGCCGGCCCAGCAGGCACAGATTCGTCTCCAGCTCGCGTCCGTGCTTGTGGCCATCATCGC
CCAGCGTCTGTTTCCAAACGGCCGCAAAAGCGGGCGGACAGCCGCGCTTGAGATTTTAATCAACAATGCGGCCGTTGCCA
ATTTGATCCGCAACAAAAAGGAGCATCAAATCGTCAACATTATGCAGACGAGCCGCAACGCGGGCATGCAGACGATGGAA
ATGAGCATCAAAGAGCTTGTGGCCGACGGACGCATCGATCCGCTTGCCGCCGAGACGTATTTGGCAGGGGAGCGTGGCGA
GCATGGCGCAATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.292

90.883

0.484
  pilT Acinetobacter baumannii strain A118

47.748

94.872

0.453
  pilT Acinetobacter baumannii D1279779

47.748

94.872

0.453
  pilT Neisseria gonorrhoeae MS11

47.605

95.157

0.453
  pilT Neisseria meningitidis 8013

47.605

95.157

0.453
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.466

92.877

0.45
  pilT Vibrio cholerae strain A1552

48.466

92.877

0.45
  pilT Pseudomonas aeruginosa PAK

48.916

92.023

0.45
  pilT Acinetobacter nosocomialis M2

47.447

94.872

0.45
  pilT Acinetobacter baylyi ADP1

47.546

92.877

0.442
  pilT Pseudomonas stutzeri DSM 10701

47.368

92.023

0.436
  pilT Legionella pneumophila strain ERS1305867

47.188

91.168

0.43
  pilT Legionella pneumophila strain Lp02

47.188

91.168

0.43
  pilU Pseudomonas stutzeri DSM 10701

42.553

93.732

0.399
  pilU Vibrio cholerae strain A1552

41.337

93.732

0.387