Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SIB52_RS09050 Genome accession   NZ_CP138962
Coordinates   1663998..1665074 (+) Length   358 a.a.
NCBI ID   WP_413404612.1    Uniprot ID   -
Organism   Synechococcus sp. MIT S9501     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1658998..1670074
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB52_RS09025 rsgA 1659108..1659989 (-) 882 WP_413404603.1 ribosome small subunit-dependent GTPase A -
  SIB52_RS09030 - 1660009..1660251 (-) 243 WP_413404605.1 sulfurtransferase TusA family protein -
  SIB52_RS09035 dnaJ 1660254..1661384 (-) 1131 WP_413404608.1 molecular chaperone DnaJ -
  SIB52_RS09040 grpE 1661427..1662164 (-) 738 WP_413406182.1 nucleotide exchange factor GrpE -
  SIB52_RS09045 pilB/pilB1 1662194..1663987 (+) 1794 WP_413404610.1 GspE/PulE family protein Machinery gene
  SIB52_RS09050 pilT 1663998..1665074 (+) 1077 WP_413404612.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB52_RS09055 - 1665078..1666502 (+) 1425 WP_413404614.1 type II secretion system F family protein -
  SIB52_RS09060 - 1666516..1667805 (-) 1290 WP_413404616.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB52_RS09065 msrB 1667735..1668244 (-) 510 WP_413404618.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIB52_RS09070 dusA 1668314..1669318 (+) 1005 WP_413404620.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SIB52_RS09075 - 1669343..1669948 (-) 606 WP_413404622.1 RNA recognition motif domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39304.98 Da        Isoelectric Point: 7.0976

>NTDB_id=906291 SIB52_RS09050 WP_413404612.1 1663998..1665074(+) (pilT) [Synechococcus sp. MIT S9501]
MELMIEDLMQELVKNGGSDLHIASGQPPYGRFSGKLRPMRDAALPEESCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSTIPSIEMLNLPPVVVETSQRPRGLVLVTGPTGSGKTTTLAALLDHINHSRAEHILTI
EDPIEFVYKSDRSLVHQRQLNEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAITAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPGQQTQIRVQLSGSLLAVFSQTLCQRNNPAPGQFGRVMAQEIMINTPAIANLIREGKTAQLYSQIQTGGELGM
QTLEKALANLVKNNTITKTEALSKASKEGELDRLISSP

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=906291 SIB52_RS09050 WP_413404612.1 1663998..1665074(+) (pilT) [Synechococcus sp. MIT S9501]
ATGGAGCTGATGATCGAGGACCTGATGCAGGAACTGGTGAAAAACGGAGGCAGTGACCTGCATATCGCCAGCGGCCAGCC
CCCCTACGGACGTTTCAGCGGCAAGCTGCGGCCGATGCGTGATGCAGCTCTGCCGGAGGAGAGCTGTAACCGACTGATCT
TCTCGATGCTCAACAACAGCCAACGCAAGACGCTTGAGCAGACATGGGAACTCGACTGCGCCTACGGACTCAAGGGCGTT
GCCCGCTTCCGGGTGAATGTGTATCGCCAGAAAGGAAGCTATGCGGCCTGCCTGCGAGCACTGGGAAGCACAATCCCAAG
CATCGAAATGCTGAATCTGCCTCCGGTGGTGGTGGAAACCAGTCAACGACCGCGGGGGCTCGTTTTGGTGACTGGACCCA
CAGGCTCAGGCAAGACAACCACCCTGGCCGCATTGCTGGATCACATCAACCACAGCCGGGCCGAACACATCCTCACCATC
GAAGATCCGATCGAGTTTGTTTACAAAAGCGATCGCAGCCTGGTGCACCAGCGTCAGCTCAATGAGGACACTCGCAGCTT
TGGAAATGCACTCAGAGCAGCCCTGCGGGAAGACCCCGACGTGATCCTGGTCGGAGAGATGCGCGACCTGGAAACCATTC
AGCTGGCCATCACCGCCGCGGAGACCGGTCACCTCGTCTTCGGCACTCTGCACACCAGTTCCGCTGCCCAGACGGTGGAC
CGCATGGTGGATGTGTTTCCTCCCGGCCAGCAGACCCAGATCCGTGTGCAACTCTCCGGAAGCCTGTTGGCGGTGTTTTC
CCAGACGCTTTGCCAACGCAACAACCCCGCTCCAGGTCAGTTCGGCCGAGTGATGGCTCAGGAAATCATGATCAACACCC
CTGCCATTGCCAACCTGATCCGCGAGGGCAAGACCGCTCAGCTGTATTCACAGATTCAGACCGGCGGCGAGCTGGGGATG
CAGACCCTGGAAAAAGCACTGGCGAATCTGGTGAAAAACAACACAATCACCAAAACGGAAGCCCTATCCAAAGCCTCCAA
AGAAGGCGAACTGGATCGGCTGATCAGCTCTCCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-246)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.666

95.251

0.511
  pilT Pseudomonas stutzeri DSM 10701

53.353

95.81

0.511
  pilT Legionella pneumophila strain Lp02

54.006

94.134

0.508
  pilT Legionella pneumophila strain ERS1305867

54.006

94.134

0.508
  pilT Acinetobacter nosocomialis M2

51.895

95.81

0.497
  pilT Acinetobacter baumannii D1279779

51.895

95.81

0.497
  pilT Acinetobacter baumannii strain A118

51.895

95.81

0.497
  pilT Neisseria meningitidis 8013

50.729

95.81

0.486
  pilT Neisseria gonorrhoeae MS11

50.437

95.81

0.483
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.143

97.765

0.48
  pilT Acinetobacter baylyi ADP1

51.807

92.737

0.48
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.294

94.972

0.478
  pilT Vibrio cholerae strain A1552

50.294

94.972

0.478
  pilU Pseudomonas stutzeri DSM 10701

39.031

98.045

0.383
  pilU Vibrio cholerae strain A1552

38.529

94.972

0.366