Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LDP65_RS16855 Genome accession   NZ_AP025152
Coordinates   3722559..3723665 (-) Length   368 a.a.
NCBI ID   WP_126608180.1    Uniprot ID   A0AAV5NVK2
Organism   Vibrio penaeicida strain TUMSAT-OK1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3717559..3728665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDP65_RS16825 - 3718304..3718804 (+) 501 WP_126608177.1 SprT family zinc-dependent metalloprotease -
  LDP65_RS16830 - 3718891..3719592 (+) 702 WP_126608178.1 endonuclease -
  LDP65_RS16835 rsmE 3719691..3720422 (+) 732 WP_126608179.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LDP65_RS16840 gshB 3720437..3721387 (+) 951 WP_101112561.1 glutathione synthase -
  LDP65_RS16845 - 3721423..3721986 (+) 564 WP_101112562.1 YqgE/AlgH family protein -
  LDP65_RS16850 ruvX 3722033..3722461 (+) 429 WP_101112582.1 Holliday junction resolvase RuvX -
  LDP65_RS16855 pilU 3722559..3723665 (-) 1107 WP_126608180.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LDP65_RS16860 pilT 3723674..3724711 (-) 1038 WP_101112564.1 type IV pilus twitching motility protein PilT Machinery gene
  LDP65_RS16865 - 3724735..3725445 (+) 711 WP_126608181.1 YggS family pyridoxal phosphate-dependent enzyme -
  LDP65_RS16870 proC 3725508..3726326 (+) 819 WP_126608182.1 pyrroline-5-carboxylate reductase -
  LDP65_RS16875 - 3726355..3726912 (+) 558 WP_126608183.1 YggT family protein -
  LDP65_RS16880 yggU 3726912..3727202 (+) 291 WP_126608184.1 DUF167 family protein YggU -
  LDP65_RS16885 - 3727226..3727657 (+) 432 WP_126608185.1 DUF4426 domain-containing protein -
  LDP65_RS16890 - 3727748..3728347 (+) 600 WP_101112570.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41289.25 Da        Isoelectric Point: 6.0176

>NTDB_id=90095 LDP65_RS16855 WP_126608180.1 3722559..3723665(-) (pilU) [Vibrio penaeicida strain TUMSAT-OK1]
MELNQVLSEMVAQKASDIYITVGAPVLLRVHGELQPLGEVLTETQAFSLLDSMMDEERRGDYRKNKESNFAIVRDVGRFR
VSAFFQRELPGAVIRRIETQIPTFEDLRLPDILQDLSIAKRGLVLVVGATGSGKSTTMAAMTGFRNQNRTGHILTVEDPI
EFVHEHNKCIITQREVGLDTDSYEIALKNSLRQAPDMILIGEIRSRETMQYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVIAQQLIRDKSGKGRHGVFEILLNTPRVSDLIRRGELHELKATMSKSNESGMKTFDQCLY
ELVVGDKITEQDAMHSADSANDLRLMLKTNRGDDFSGGALDGVKIDMN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=90095 LDP65_RS16855 WP_126608180.1 3722559..3723665(-) (pilU) [Vibrio penaeicida strain TUMSAT-OK1]
ATGGAACTGAATCAAGTCTTATCTGAAATGGTGGCTCAGAAAGCCTCAGATATCTACATTACCGTTGGTGCACCTGTTTT
GCTGCGAGTCCATGGCGAATTGCAGCCATTGGGAGAAGTGTTGACGGAAACACAAGCCTTTTCCCTGCTCGATAGCATGA
TGGATGAAGAGCGACGAGGCGATTACCGTAAAAATAAAGAGTCCAACTTCGCGATTGTTCGCGATGTGGGTCGCTTTAGG
GTCAGTGCTTTTTTCCAGCGTGAATTACCAGGAGCGGTAATACGCCGAATTGAAACTCAAATACCTACCTTTGAGGATCT
TCGATTACCCGATATCTTACAAGACTTGTCTATTGCTAAACGTGGTTTGGTGCTGGTGGTTGGTGCTACAGGTTCAGGTA
AATCAACCACCATGGCAGCCATGACAGGCTTTCGTAATCAAAACCGAACAGGGCACATTCTGACGGTTGAGGATCCTATT
GAATTTGTTCACGAGCACAACAAGTGCATCATCACACAACGTGAAGTTGGGCTGGATACCGATTCTTATGAAATTGCGTT
GAAGAACTCGCTTCGTCAAGCGCCAGATATGATCTTGATTGGTGAAATTCGTTCACGAGAGACCATGCAATACGCCATGA
CATTTGCTGAGACTGGGCACTTGTGTATGGCGACTCTGCACGCCAATAACGCCAACCAAGCACTAGAGCGAATTCTTCAT
TTAGTGCCTAAAGAGCAAAAAGAGCAATTCCTATTCGATCTCTCCATGAACCTCCGAGGAGTGATCGCTCAGCAATTGAT
CCGGGATAAGAGCGGTAAAGGGCGTCATGGGGTGTTTGAGATCTTATTGAATACGCCAAGGGTATCTGATCTTATTCGTC
GCGGAGAATTGCATGAGCTAAAAGCGACGATGTCGAAATCGAATGAATCGGGTATGAAAACCTTCGATCAGTGTTTGTAC
GAGCTGGTGGTTGGCGACAAGATTACAGAGCAAGACGCCATGCACAGCGCCGATTCGGCAAACGATTTAAGACTAATGCT
GAAAACCAACCGTGGAGACGATTTCTCTGGTGGCGCATTGGACGGTGTGAAGATCGATATGAATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

78.747

99.728

0.785
  pilU Pseudomonas stutzeri DSM 10701

56.857

95.109

0.541
  pilU Acinetobacter baylyi ADP1

53.561

95.38

0.511
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.901

88.043

0.378
  pilT Legionella pneumophila strain Lp02

40.708

92.12

0.375
  pilT Legionella pneumophila strain ERS1305867

40.708

92.12

0.375
  pilT Vibrio cholerae strain A1552

41.018

90.761

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.761

0.372
  pilT Pseudomonas aeruginosa PAK

40.118

92.12

0.37
  pilT Acinetobacter baylyi ADP1

41.846

88.315

0.37
  pilT Pseudomonas stutzeri DSM 10701

39.233

92.12

0.361

Multiple sequence alignment