Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   AABJ99_RS19975 Genome accession   NZ_AP027764
Coordinates   4183531..4184913 (-) Length   460 a.a.
NCBI ID   WP_001029698.1    Uniprot ID   A0A2T3RLH6
Organism   Escherichia coli strain 2017-02-2CC     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4178531..4189913
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABJ99_RS19955 (VEE76_38150) ettA 4179845..4181512 (+) 1668 WP_000046749.1 energy-dependent translational throttle protein EttA -
  AABJ99_RS19960 (VEE76_38160) nadS 4181568..4181852 (-) 285 WP_000007436.1 NadS family protein -
  AABJ99_RS19965 (VEE76_38170) - 4181854..4182186 (-) 333 WP_000513551.1 type II toxin-antitoxin system RelE/ParE family toxin -
  AABJ99_RS19970 (VEE76_38180) nadR 4182278..4183510 (-) 1233 WP_338387413.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  AABJ99_RS19975 (VEE76_38190) radA/sms 4183531..4184913 (-) 1383 WP_001029698.1 DNA repair protein RadA Machinery gene
  AABJ99_RS19980 (VEE76_38200) serB 4184962..4185930 (-) 969 WP_001132956.1 phosphoserine phosphatase -
  AABJ99_RS19985 (VEE76_38210) ytjB 4186036..4186680 (+) 645 WP_000124615.1 YtjB family periplasmic protein -
  AABJ99_RS19990 (VEE76_38220) lplA 4186708..4187724 (+) 1017 WP_000105850.1 lipoate--protein ligase LplA -
  AABJ99_RS19995 (VEE76_38230) - 4187756..4188019 (+) 264 WP_039020380.1 helix-turn-helix transcriptional regulator -
  AABJ99_RS20000 (VEE76_38240) deoD 4188180..4188899 (-) 720 WP_000224877.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49442.02 Da        Isoelectric Point: 7.1936

>NTDB_id=90096 AABJ99_RS19975 WP_001029698.1 4183531..4184913(-) (radA/sms) [Escherichia coli strain 2017-02-2CC]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=90096 AABJ99_RS19975 WP_001029698.1 4183531..4184913(-) (radA/sms) [Escherichia coli strain 2017-02-2CC]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCAGATTATCCGCGCTGGCAGGGGCAGTGCAGCGC
CTGCCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGTGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTTTCCGATATCAGCCTTGAGGAGTTGCCGCGTTTTTCAACCGGA
TTTAAAGAGTTCGACCGCGTATTAGGCGGTGGGGTGGTGCCGGGAAGCGCCATTCTGATTGGCGGTAATCCAGGCGCAGG
GAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTATATGTCACTGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTCGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAA
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTTATTGACTCCATCCAGGTGATGCATATGGCGGATGT
TCAGTCATCGCCTGGCAGCGTGGCACAGGTGCGTGAAACGGCGGCCTATCTGACGCGCTTCGCCAAAACGCGTGGCGTGG
CGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCAGGCCCTAAAGTGCTGGAACACTGTATCGACTGCTCG
GTGCTTCTGGACGGCGATGCCGACTCCCGTTTCCGCACCTTACGCAGCCATAAAAACCGTTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACTGAACAGGGGCTGCGTGAAGTTAGCAACCCTTCCGCCATCTTTTTAAGCCGCGGCGATGAAG
TGACCTCTGGTAGTTCAGTGATGGTGGTATGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCGATGATGGCGAATCCACGCCGCGTCGCAGTAGGGCTGGAGCAAAACCGTCTGGCAATCCTGCTGGCGGTGCTTCACCG
TCACGGTGGGTTGCAAATGGCCGATCAGGATGTGTTTGTTAACGTGGTCGGTGGCGTGAAGGTAACCGAAACCAGTGCCG
ATTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCACTACCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGCCAGGAGCGGATTTCTGAAGCGGCGAAACATGGTTTTCGGCGGGC
GATTGTTCCGGCAGCCAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3RLH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43

Multiple sequence alignment