NTDB

Overview

Name	comGA/cglA/cilD	Type	Machinery gene
Locus tag	R4712_RS06250	Genome accession	NZ_CP137110
Coordinates	1240452..1241393 (+)	Length	313 a.a.
NCBI ID	WP_016398028.1	Uniprot ID	A0A0U0BVC9
Organism	Streptococcus pneumoniae strain 16H2041
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1235452..1246393

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R4712_RS06230	-	1235518..1237335 (+)	1818	WP_044792290.1	acyltransferase family protein	-
R4712_RS06235	nagA	1237488..1238639 (+)	1152	WP_001134457.1	N-acetylglucosamine-6-phosphate deacetylase	-
R4712_RS06240	-	1238802..1239860 (+)	1059	WP_044812807.1	zinc-dependent alcohol dehydrogenase family protein	-
R4712_RS06245	-	1240011..1240376 (+)	366	WP_000286415.1	DUF1033 family protein	-
R4712_RS06250	comGA/cglA/cilD	1240452..1241393 (+)	942	WP_016398028.1	competence type IV pilus ATPase ComGA	Machinery gene
R4712_RS06255	comGB/cglB	1241341..1242357 (+)	1017	WP_074196785.1	competence type IV pilus assembly protein ComGB	Machinery gene
R4712_RS06260	comGC/cglC	1242359..1242685 (+)	327	WP_000738626.1	comG operon protein ComGC	Machinery gene
R4712_RS06265	comGD/cglD	1242678..1243082 (+)	405	WP_288176807.1	competence type IV pilus minor pilin ComGD	Machinery gene
R4712_RS06270	comGE/cglE	1243045..1243347 (+)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
R4712_RS06275	comGF/cglF	1243310..1243771 (+)	462	WP_001864569.1	competence type IV pilus minor pilin ComGF	Machinery gene
R4712_RS06280	comGG/cglG	1243749..1244162 (+)	414	WP_001864570.1	competence type IV pilus minor pilin ComGG	Machinery gene
R4712_RS06285	-	1244299..1244893 (+)	595	Protein_1230	class I SAM-dependent methyltransferase	-
R4712_RS06290	comYH	1244954..1245907 (+)	954	WP_001866278.1	class I SAM-dependent methyltransferase	Machinery gene

Sequence

Protein

Download Length: 313 a.a. Molecular weight: 35546.46 Da Isoelectric Point: 6.0083

                                >NTDB_id=895872 R4712_RS06250 WP_016398028.1 1240452..1241393(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 16H2041]

MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA

YDQKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ

VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY

ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide

Download Length: 942 bp

                                >NTDB_id=895872 R4712_RS06250 WP_016398028.1 1240452..1241393(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 16H2041]

ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATCTATTTTGTCCCTAAGTT

AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG

TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC

TATGACCAGAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT

GCACGATGAGGAGCAGGACCTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT

ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA

GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC

CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG

CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT

GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG

GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC

TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0U0BVC9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	99.681	100	0.997
comGA/cglA/cilD	Streptococcus pneumoniae D39	99.681	100	0.997
comGA/cglA/cilD	Streptococcus pneumoniae R6	99.681	100	0.997
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	99.681	100	0.997
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	96.166	100	0.962
comYA	Streptococcus gordonii str. Challis substr. CH1	78.065	99.042	0.773
comYA	Streptococcus mutans UA159	65.916	99.361	0.655
comYA	Streptococcus mutans UA140	65.916	99.361	0.655
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	62.581	99.042	0.62
comGA	Lactococcus lactis subsp. cremoris KW2	54.808	99.681	0.546
comGA	Latilactobacillus sakei subsp. sakei 23K	42.642	84.665	0.361

Detailed information NTG0895872