Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   R4712_RS06255 Genome accession   NZ_CP137110
Coordinates   1241341..1242357 (+) Length   338 a.a.
NCBI ID   WP_074196785.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 16H2041     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1236341..1247357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4712_RS06235 nagA 1237488..1238639 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  R4712_RS06240 - 1238802..1239860 (+) 1059 WP_044812807.1 zinc-dependent alcohol dehydrogenase family protein -
  R4712_RS06245 - 1240011..1240376 (+) 366 WP_000286415.1 DUF1033 family protein -
  R4712_RS06250 comGA/cglA/cilD 1240452..1241393 (+) 942 WP_016398028.1 competence type IV pilus ATPase ComGA Machinery gene
  R4712_RS06255 comGB/cglB 1241341..1242357 (+) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4712_RS06260 comGC/cglC 1242359..1242685 (+) 327 WP_000738626.1 comG operon protein ComGC Machinery gene
  R4712_RS06265 comGD/cglD 1242678..1243082 (+) 405 WP_288176807.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4712_RS06270 comGE/cglE 1243045..1243347 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4712_RS06275 comGF/cglF 1243310..1243771 (+) 462 WP_001864569.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4712_RS06280 comGG/cglG 1243749..1244162 (+) 414 WP_001864570.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4712_RS06285 - 1244299..1244893 (+) 595 Protein_1230 class I SAM-dependent methyltransferase -
  R4712_RS06290 comYH 1244954..1245907 (+) 954 WP_001866278.1 class I SAM-dependent methyltransferase Machinery gene
  R4712_RS06295 - 1245958..1247148 (+) 1191 WP_000167766.1 acetate kinase -
  R4712_RS06300 - 1247149..1247280 (+) 132 WP_000768904.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38392.40 Da        Isoelectric Point: 9.4802

>NTDB_id=895873 R4712_RS06255 WP_074196785.1 1241341..1242357(+) (comGB/cglB) [Streptococcus pneumoniae strain 16H2041]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEV
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=895873 R4712_RS06255 WP_074196785.1 1241341..1242357(+) (comGB/cglB) [Streptococcus pneumoniae strain 16H2041]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAGTC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

100

100

1
  comGB/cglB Streptococcus pneumoniae D39

100

100

1
  comGB/cglB Streptococcus pneumoniae R6

100

100

1
  comGB/cglB Streptococcus pneumoniae TIGR4

100

100

1
  comGB/cglB Streptococcus mitis SK321

95.266

100

0.953
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

71.131

99.408

0.707
  comYB Streptococcus mutans UA140

57.547

94.083

0.541
  comYB Streptococcus mutans UA159

57.547

94.083

0.541
  comGB Lactococcus lactis subsp. cremoris KW2

51.198

98.817

0.506