Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RY019_RS17720 Genome accession   NZ_CP136540
Coordinates   3718913..3719893 (-) Length   326 a.a.
NCBI ID   WP_048966719.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain E378246     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3713913..3724893
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RY019_RS17690 (RY019_17650) - 3714790..3715287 (+) 498 WP_032621896.1 SprT family zinc-dependent metalloprotease -
  RY019_RS17695 (RY019_17655) endA 3715382..3716089 (+) 708 WP_022649352.1 deoxyribonuclease I -
  RY019_RS17700 (RY019_17660) rsmE 3716141..3716872 (+) 732 WP_003860033.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RY019_RS17705 (RY019_17665) gshB 3716904..3717851 (+) 948 WP_003860032.1 glutathione synthase -
  RY019_RS17710 (RY019_17670) - 3717926..3718486 (+) 561 WP_022649353.1 YqgE/AlgH family protein -
  RY019_RS17715 (RY019_17675) ruvX 3718486..3718902 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  RY019_RS17720 (RY019_17680) pilT 3718913..3719893 (-) 981 WP_048966719.1 type IV pilus twitching motility protein PilT Machinery gene
  RY019_RS17725 (RY019_17685) - 3719911..3720612 (+) 702 WP_048966720.1 YggS family pyridoxal phosphate-dependent enzyme -
  RY019_RS17730 (RY019_17690) - 3720634..3721200 (+) 567 WP_400174505.1 YggT family protein -
  RY019_RS17735 (RY019_17695) yggU 3721197..3721493 (+) 297 WP_022649356.1 DUF167 family protein YggU -
  RY019_RS17740 (RY019_17700) - 3721497..3722090 (+) 594 WP_022649357.1 XTP/dITP diphosphatase -
  RY019_RS17745 (RY019_17705) hemW 3722083..3723231 (+) 1149 WP_022649358.1 radical SAM family heme chaperone HemW -
  RY019_RS17750 (RY019_17710) - 3723288..3723650 (+) 363 WP_022651832.1 endonuclease domain-containing protein -
  RY019_RS17755 (RY019_17715) - 3723763..3724479 (-) 717 WP_003862419.1 DUF2884 domain-containing protein -
  RY019_RS17760 (RY019_17720) - 3724537..3724863 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35800.80 Da        Isoelectric Point: 6.6606

>NTDB_id=891526 RY019_RS17720 WP_048966719.1 3718913..3719893(-) (pilT) [Enterobacter hormaechei strain E378246]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLADRQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSQRCLIRQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=891526 RY019_RS17720 WP_048966719.1 3718913..3719893(-) (pilT) [Enterobacter hormaechei strain E378246]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ATGAACAGCAGGGCGCATGGTGGGCAAATGGACAGGTGGATTTTGCCGTTACCCTCGCAGACCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTACGCCTGTTGCCGCTAACGTGTCCGCAGCTCTCTGCGCTAGG
CGTGCCGCGAGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTTCTCAATCACCATACGGACGGGCACATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGTCAACGTTGCCTCATCCGGCAGCGGGAGATAGGCCAGCATAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGTGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCTGAGACTGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGATACC
TTCCCGGCGCAGGAGAAAGATCCGGTTCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTACTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGTA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.653

99.08

0.433
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38