NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	RO483_RS12540	Genome accession	NZ_CP135968
Coordinates	2725695..2726144 (+)	Length	149 a.a.
NCBI ID	WP_065645564.1	Uniprot ID	-
Organism	Vibrio alginolyticus strain S10
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2720695..2731144

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RO483_RS12525 (RO483_12525)	pdhR	2722728..2723495 (-)	768	WP_005379989.1	pyruvate dehydrogenase complex transcriptional repressor PdhR	-
RO483_RS12530 (RO483_12530)	ampD	2723901..2724452 (-)	552	WP_042521337.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
RO483_RS12535 (RO483_12535)	nadC	2724545..2725432 (+)	888	WP_258664543.1	carboxylating nicotinate-nucleotide diphosphorylase	-
RO483_RS12540 (RO483_12540)	pilA	2725695..2726144 (+)	450	WP_065645564.1	pilin	Machinery gene
RO483_RS12545 (RO483_12545)	pilB	2726148..2727833 (+)	1686	WP_315956559.1	type IV-A pilus assembly ATPase PilB	Machinery gene
RO483_RS12550 (RO483_12550)	pilC	2727857..2729080 (+)	1224	WP_017635918.1	type II secretion system F family protein	Machinery gene
RO483_RS12555 (RO483_12555)	pilD	2729145..2730014 (+)	870	WP_054576331.1	A24 family peptidase	Machinery gene
RO483_RS12560 (RO483_12560)	coaE	2730015..2730629 (+)	615	WP_054576332.1	dephospho-CoA kinase	-

Sequence

Protein

Download Length: 149 a.a. Molecular weight: 15559.72 Da Isoelectric Point: 7.7816

                                >NTDB_id=887923 RO483_RS12540 WP_065645564.1 2725695..2726144(+) (pilA) [Vibrio alginolyticus strain S10]

MKNSKQKKQQGFTLIELMIVVAVIGVLAAIAIPQYQNYVAKSELGAGLATITSVRTNVEDYIVTNGSFPDGTTAGQLTTD

LGVIQPNNGAITFDDANSNILLTFAGSGNSPDVNNAKIALNRAAGGTWTCKATLDAKLLPKSCSKDANL

Nucleotide

Download Length: 450 bp

                                >NTDB_id=887923 RO483_RS12540 WP_065645564.1 2725695..2726144(+) (pilA) [Vibrio alginolyticus strain S10]

ATGAAAAACAGTAAACAGAAAAAACAACAGGGTTTTACTCTGATTGAATTAATGATTGTGGTGGCCGTTATTGGTGTTTT

AGCAGCGATTGCTATCCCTCAATACCAAAACTATGTGGCCAAGTCAGAGTTGGGGGCTGGACTAGCAACCATAACCTCAG

TTCGAACTAATGTTGAAGATTACATTGTTACAAATGGTAGTTTTCCTGATGGTACAACTGCTGGCCAGCTAACAACTGAC

TTAGGAGTGATTCAGCCAAACAATGGTGCAATAACTTTTGATGACGCCAATAGCAACATCCTGTTAACTTTTGCCGGTTC

TGGAAATAGCCCTGACGTAAATAATGCAAAAATAGCACTAAACAGAGCGGCTGGTGGTACATGGACATGTAAAGCCACCC

TAGATGCTAAACTACTTCCAAAATCATGCTCTAAGGATGCTAATTTATAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	52.288	100	0.537
pilA	Vibrio cholerae strain A1552	52.288	100	0.537
pilA	Vibrio cholerae C6706	52.288	100	0.537
pilA	Pseudomonas aeruginosa PAK	45.638	100	0.456
pilA	Vibrio parahaemolyticus RIMD 2210633	47.059	91.275	0.43
pilA/pilAI	Pseudomonas stutzeri DSM 10701	41.667	96.644	0.403
pilA	Acinetobacter baumannii strain A118	40.58	92.617	0.376
pilA2	Legionella pneumophila str. Paris	39.855	92.617	0.369
pilA	Haemophilus influenzae 86-028NP	36.242	100	0.362
pilA	Haemophilus influenzae Rd KW20	35.294	100	0.362
pilA	Acinetobacter nosocomialis M2	37.762	95.973	0.362
pilA/pilA1	Eikenella corrodens VA1	33.333	100	0.362
pilA2	Legionella pneumophila strain ERS1305867	39.13	92.617	0.362

Detailed information NTG0887923