Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RO483_RS12545 Genome accession   NZ_CP135968
Coordinates   2726148..2727833 (+) Length   561 a.a.
NCBI ID   WP_315956559.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain S10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2721148..2732833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RO483_RS12525 (RO483_12525) pdhR 2722728..2723495 (-) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  RO483_RS12530 (RO483_12530) ampD 2723901..2724452 (-) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RO483_RS12535 (RO483_12535) nadC 2724545..2725432 (+) 888 WP_258664543.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RO483_RS12540 (RO483_12540) pilA 2725695..2726144 (+) 450 WP_065645564.1 pilin Machinery gene
  RO483_RS12545 (RO483_12545) pilB 2726148..2727833 (+) 1686 WP_315956559.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RO483_RS12550 (RO483_12550) pilC 2727857..2729080 (+) 1224 WP_017635918.1 type II secretion system F family protein Machinery gene
  RO483_RS12555 (RO483_12555) pilD 2729145..2730014 (+) 870 WP_054576331.1 A24 family peptidase Machinery gene
  RO483_RS12560 (RO483_12560) coaE 2730015..2730629 (+) 615 WP_054576332.1 dephospho-CoA kinase -
  RO483_RS12565 (RO483_12565) zapD 2730657..2731397 (+) 741 WP_005373086.1 cell division protein ZapD -
  RO483_RS12570 (RO483_12570) yacG 2731556..2731750 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  RO483_RS12575 (RO483_12575) rplS 2732063..2732416 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62133.98 Da        Isoelectric Point: 5.2979

>NTDB_id=887924 RO483_RS12545 WP_315956559.1 2726148..2727833(+) (pilB) [Vibrio alginolyticus strain S10]
MLTNLSTVLRQKGVLTFSQEESLIEQVKASGISMPEALLSSGLFTSSELAEHLSSLFGLNQPELSQYEYASLCQQLGLRE
LITRHNALPLQRTSSTLLLAIADPTNQQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRALGQEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKQSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=887924 RO483_RS12545 WP_315956559.1 2726148..2727833(+) (pilB) [Vibrio alginolyticus strain S10]
ATGTTAACCAACCTATCAACCGTTCTTCGTCAAAAAGGAGTACTGACCTTTTCCCAAGAAGAATCGTTAATAGAGCAAGT
CAAAGCTTCTGGCATTTCGATGCCAGAGGCTTTACTCAGTTCTGGATTATTTACGTCAAGCGAACTCGCTGAGCACTTAA
GTTCTCTCTTTGGTTTAAATCAGCCCGAGTTATCTCAGTACGAGTATGCTTCGCTTTGTCAGCAACTTGGCCTTAGGGAA
TTAATCACGCGGCATAACGCACTCCCACTCCAGCGTACGTCTTCAACGTTATTATTAGCGATTGCCGACCCCACCAACCA
ACAAGCAGAAGATGATTTTCGCTTTGCGACAGGATTGCAAGTAGAACTTGTTCTCGCTGATTTTCGCGAGCTGACCGCTG
CGATTCGGCGCTTATATGGCCGCGCACTTGGGCAAGAAAAGTCTGGGTTAAAAGAGATCAACCAAGAAGAGTTAGCCAAC
CTAGTTGACGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGATGAGTCTCCTGTTAGCCGTTACATCAA
CCAAATTCTGTTGGACGCTGTGCGCAAAGGTGCATCCGATATCCACTTTGAGCCTTATGAAAAGATGTACCGCGTGCGCC
TACGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTAAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAACTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCAAT
TGATATGCGTGTATCCACGCTACCGACTCTATTCGGAGAGAAAATTGTACTACGACTGCTCGACAGCAGCTCTGCGTCAC
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGCCCAACCGGGAGCGGAAAAACCGTTTCTCTGTACACCGGGCTGAGTATCCTCAATAAACCAGAAATCAA
CATTTCTACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCCCTGCGCTCTTTTCTTCGTCAAGACCCCGATGTGGTCATGGTCGGTGAGATTCGTGACTTAGACACT
GCGGAAATCGCCATAAAAGCATCGCAAACCGGTCACTTAGTACTTTCAACCTTGCATACCAATTCTGCGGCTGAAACGGT
TATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCATCTCTTAGTTTGATTATTGCCCAGCGCTTAGCCC
GTAAACTGTGCTCGCACTGCAAACAATCACAAGAGCTTACCGTTCAGCTACAACATCTCGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGCAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGCGCGTCGGTACATGAACTAGAAAAACTCGCCATCGCAAATGGTA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

95.009

100

0.95
  pilB Vibrio campbellii strain DS40M4

90.909

100

0.909
  pilB Vibrio cholerae strain A1552

74.021

100

0.742
  pilB Acinetobacter baumannii D1279779

51.619

93.583

0.483
  pilB Legionella pneumophila strain ERS1305867

49.168

96.435

0.474
  pilB Acinetobacter baylyi ADP1

50.29

92.157

0.463
  pilF Neisseria gonorrhoeae MS11

44.326

100

0.446
  pilB/pilB1 Synechocystis sp. PCC 6803

37.838

100

0.399
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.684

100

0.371
  pilF Thermus thermophilus HB27

37.294

97.504

0.364