Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RRL13_RS18780 Genome accession   NZ_CP135498
Coordinates   3978944..3979951 (-) Length   335 a.a.
NCBI ID   WP_315686743.1    Uniprot ID   -
Organism   Enterobacter cloacae strain isolateB     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3973944..3984951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RRL13_RS18745 (RRL13_18745) - 3974066..3974563 (+) 498 WP_182062367.1 SprT family zinc-dependent metalloprotease -
  RRL13_RS18750 (RRL13_18750) endA 3974658..3975365 (+) 708 WP_315686741.1 deoxyribonuclease I -
  RRL13_RS18755 (RRL13_18755) rsmE 3975418..3976149 (+) 732 WP_182062369.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RRL13_RS18760 (RRL13_18760) gshB 3976169..3977116 (+) 948 WP_182062370.1 glutathione synthase -
  RRL13_RS18765 (RRL13_18765) - 3977191..3977751 (+) 561 WP_182062371.1 YqgE/AlgH family protein -
  RRL13_RS18770 (RRL13_18770) ruvX 3977751..3978167 (+) 417 WP_014071696.1 Holliday junction resolvase RuvX -
  RRL13_RS18775 (RRL13_18775) - 3978161..3978883 (-) 723 WP_182062372.1 IclR family transcriptional regulator -
  RRL13_RS18780 (RRL13_18780) pilT 3978944..3979951 (-) 1008 WP_315686743.1 type IV pilus twitching motility protein PilT Machinery gene
  RRL13_RS18785 (RRL13_18785) - 3979969..3980673 (+) 705 WP_182062374.1 YggS family pyridoxal phosphate-dependent enzyme -
  RRL13_RS18790 (RRL13_18790) - 3980692..3981258 (+) 567 WP_182062375.1 YggT family protein -
  RRL13_RS18795 (RRL13_18795) yggU 3981255..3981551 (+) 297 WP_182062376.1 DUF167 family protein YggU -
  RRL13_RS18800 (RRL13_18800) - 3981555..3982148 (+) 594 WP_182062377.1 XTP/dITP diphosphatase -
  RRL13_RS18805 (RRL13_18805) hemW 3982141..3983289 (+) 1149 WP_182062378.1 radical SAM family heme chaperone HemW -
  RRL13_RS18810 (RRL13_18810) - 3983346..3983684 (+) 339 WP_182062379.1 endonuclease domain-containing protein -
  RRL13_RS18815 (RRL13_18815) - 3983736..3984455 (-) 720 WP_315686749.1 DUF2884 domain-containing protein -
  RRL13_RS18820 (RRL13_18820) - 3984509..3984835 (-) 327 WP_182062380.1 YggL family protein -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 36718.84 Da        Isoelectric Point: 6.8755

>NTDB_id=886894 RRL13_RS18780 WP_315686743.1 3978944..3979951(-) (pilT) [Enterobacter cloacae strain isolateB]
MVMEEIVALSVKHNVSDLHLCSDSPPRWRRAGTLEPAPFPPPDITQLLKTWLNDEQQGTWWANGQVDFAVTLTSQQRLRG
SAFRHINGASLTLRLLPASCPQLSALGAPRVLPVLLDNDSGLILVTGATGSGKSTTLAAMVDHLNRHCDKHILTLEDPVE
FIYQSDRCLIHQREIGQHCPSFAEALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLFRDVQGGRVALYELLVNTSASANLIREGKTWQLPGIIQTGQQAGMQNFDQSFAER
RAQGRLHERSAVAPG

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=886894 RRL13_RS18780 WP_315686743.1 3978944..3979951(-) (pilT) [Enterobacter cloacae strain isolateB]
GTGGTAATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTGTCGGATCTACACCTGTGTAGTGATTCACCGCCACG
CTGGCGCAGGGCAGGTACGCTTGAACCTGCGCCATTCCCGCCGCCCGATATTACGCAGCTATTAAAAACGTGGCTTAACG
ACGAACAGCAGGGAACCTGGTGGGCAAATGGGCAGGTGGATTTTGCAGTGACGCTCACCAGCCAGCAGCGTTTACGCGGC
AGTGCATTCCGCCACATTAACGGGGCCTCACTCACGCTCAGACTCTTGCCGGCGAGCTGCCCACAGCTTTCGGCGCTGGG
TGCGCCGCGTGTGCTCCCGGTACTGCTGGACAACGACAGTGGTTTGATTCTGGTGACAGGGGCAACCGGAAGTGGCAAAT
CCACCACGCTTGCGGCAATGGTGGATCATCTTAATCGCCACTGCGATAAACACATTCTGACGCTGGAAGACCCGGTTGAG
TTTATTTATCAAAGCGATCGCTGTCTTATCCATCAACGGGAAATAGGCCAACACTGTCCTTCATTTGCAGAGGCGCTTCG
CGCCGCATTACGTGAAGATCCTGATGTGATTTTGTTGGGGGAGCTTCGCGACAGCGAAACGATCAGGCTGGCCCTTACGG
CAGCGGAAACCGGCCATCTGGTGTTGGCAACACTGCATACGCGTGGTGCTGCGCAGGCAATAGAGCGCCTGGTCGATACC
TTTCCGGCACAGGAAAAAGATCCGGTGCGCAATCAACTGGCGGGTAGTCTTCGCGCGGTACTGGCGCAAAAGCTCTTTCG
GGATGTTCAGGGAGGGCGCGTGGCACTGTACGAACTGCTGGTGAATACTTCAGCGTCGGCAAACCTTATCCGTGAGGGCA
AAACCTGGCAATTGCCTGGTATTATCCAGACCGGGCAACAGGCGGGGATGCAGAATTTTGACCAAAGCTTTGCCGAACGC
AGAGCGCAAGGGCGGCTTCACGAAAGGTCGGCGGTTGCGCCGGGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.305

97.91

0.493
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.305

97.91

0.493
  pilT Pseudomonas stutzeri DSM 10701

47.305

99.701

0.472
  pilT Acinetobacter baumannii strain A118

46.108

99.701

0.46
  pilT Pseudomonas aeruginosa PAK

46.108

99.701

0.46
  pilT Acinetobacter baumannii D1279779

46.108

99.701

0.46
  pilT Neisseria meningitidis 8013

46.951

97.91

0.46
  pilT Acinetobacter baylyi ADP1

46.789

97.612

0.457
  pilT Neisseria gonorrhoeae MS11

46.646

97.91

0.457
  pilT Acinetobacter nosocomialis M2

45.808

99.701

0.457
  pilT Legionella pneumophila strain ERS1305867

47.205

96.119

0.454
  pilT Legionella pneumophila strain Lp02

47.205

96.119

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.188

95.522

0.403
  pilU Pseudomonas stutzeri DSM 10701

38.393

100

0.385
  pilU Vibrio cholerae strain A1552

39.385

97.015

0.382