Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RP424_RS03030 Genome accession   NZ_CP135270
Coordinates   624202..625182 (+) Length   326 a.a.
NCBI ID   WP_023314823.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain IMT49658-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 619202..630182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RP424_RS02990 - 619232..619558 (+) 327 WP_003862421.1 YggL family protein -
  RP424_RS02995 - 619616..620332 (+) 717 WP_003862419.1 DUF2884 domain-containing protein -
  RP424_RS03000 - 620445..620807 (-) 363 WP_022651832.1 endonuclease domain-containing protein -
  RP424_RS03005 hemW 620864..622012 (-) 1149 WP_023323732.1 radical SAM family heme chaperone HemW -
  RP424_RS03010 - 622005..622598 (-) 594 WP_022651831.1 XTP/dITP diphosphatase -
  RP424_RS03015 yggU 622602..622898 (-) 297 WP_003860023.1 DUF167 family protein YggU -
  RP424_RS03020 - 622895..623461 (-) 567 WP_003860024.1 YggT family protein -
  RP424_RS03025 - 623486..624184 (-) 699 WP_023314824.1 YggS family pyridoxal phosphate-dependent enzyme -
  RP424_RS03030 pilT 624202..625182 (+) 981 WP_023314823.1 type IV pilus twitching motility protein PilT Machinery gene
  RP424_RS03035 ruvX 625193..625609 (-) 417 WP_003860030.1 Holliday junction resolvase RuvX -
  RP424_RS03040 - 625609..626169 (-) 561 WP_022649353.1 YqgE/AlgH family protein -
  RP424_RS03045 gshB 626244..627191 (-) 948 WP_003860032.1 glutathione synthase -
  RP424_RS03050 rsmE 627219..627950 (-) 732 WP_023314822.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RP424_RS03055 endA 628002..628709 (-) 708 WP_003860034.1 deoxyribonuclease I -
  RP424_RS03060 - 628804..629301 (-) 498 WP_023314821.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35608.58 Da        Isoelectric Point: 6.2335

>NTDB_id=886346 RP424_RS03030 WP_023314823.1 624202..625182(+) (pilT) [Enterobacter hormaechei strain IMT49658-1]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGLHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRV

Nucleotide


Download         Length: 981 bp        

>NTDB_id=886346 RP424_RS03030 WP_023314823.1 624202..625182(+) (pilT) [Enterobacter hormaechei strain IMT49658-1]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCATTATTAAAAGCGTGGCTCAATG
ACGAACAGCAGGGCGCCTGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTGCGCCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACTCTGGCCGCGATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCTGCACAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGCGCTCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAACTGGCGGGAAGCCTGCGTGCGGTTCTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGGTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.456

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.456

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

47.72

100

0.482
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Neisseria meningitidis 8013

46.97

100

0.475
  pilT Acinetobacter baumannii D1279779

46.809

100

0.472
  pilT Acinetobacter baumannii strain A118

46.809

100

0.472
  pilT Pseudomonas aeruginosa PAK

46.809

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.667

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38