Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RMN59_RS17795 Genome accession   NZ_CP134705
Coordinates   3763667..3764647 (-) Length   326 a.a.
NCBI ID   WP_032669250.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. hormaechei strain Naas 2022_1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3758667..3769647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RMN59_RS17765 (RMN59_17765) - 3759544..3760041 (+) 498 WP_311380271.1 SprT family zinc-dependent metalloprotease -
  RMN59_RS17770 (RMN59_17770) endA 3760136..3760843 (+) 708 WP_006811921.1 deoxyribonuclease I -
  RMN59_RS17775 (RMN59_17775) rsmE 3760895..3761626 (+) 732 WP_311380272.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RMN59_RS17780 (RMN59_17780) gshB 3761658..3762605 (+) 948 WP_006811923.1 glutathione synthase -
  RMN59_RS17785 (RMN59_17785) - 3762680..3763240 (+) 561 WP_006811924.1 YqgE/AlgH family protein -
  RMN59_RS17790 (RMN59_17790) ruvX 3763240..3763656 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  RMN59_RS17795 (RMN59_17795) pilT 3763667..3764647 (-) 981 WP_032669250.1 type IV pilus twitching motility protein PilT Machinery gene
  RMN59_RS17800 (RMN59_17800) - 3764665..3765366 (+) 702 WP_047355180.1 YggS family pyridoxal phosphate-dependent enzyme -
  RMN59_RS17805 (RMN59_17805) - 3765388..3765954 (+) 567 WP_006811928.1 YggT family protein -
  RMN59_RS17810 (RMN59_17810) yggU 3765951..3766247 (+) 297 WP_003860023.1 DUF167 family protein YggU -
  RMN59_RS17815 (RMN59_17815) - 3766251..3766844 (+) 594 WP_311380273.1 XTP/dITP diphosphatase -
  RMN59_RS17820 (RMN59_17820) hemW 3766837..3767985 (+) 1149 WP_311380274.1 radical SAM family heme chaperone HemW -
  RMN59_RS17825 (RMN59_17825) - 3768021..3768140 (+) 120 Protein_3480 DUF559 domain-containing protein -
  RMN59_RS17830 (RMN59_17830) - 3768243..3768959 (-) 717 WP_006811932.1 DUF2884 domain-containing protein -
  RMN59_RS17835 (RMN59_17835) - 3769016..3769342 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35691.71 Da        Isoelectric Point: 6.4131

>NTDB_id=883032 RMN59_RS17795 WP_032669250.1 3763667..3764647(-) (pilT) [Enterobacter hormaechei subsp. hormaechei strain Naas 2022_1]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMLGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=883032 RMN59_RS17795 WP_032669250.1 3763667..3764647(-) (pilT) [Enterobacter hormaechei subsp. hormaechei strain Naas 2022_1]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCGCCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCAGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTCAAGCATATGCTCGGCGTTTCGATCGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAACTGCTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCACCACACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGGTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCCTCCTTTGCCGAGGCCCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTGGGGGAGTTGCGCGACAGCGAAACAATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCACACGCGCGGTGCGGCGCAGGCAATTGAGCGCCTGGTCGATACC
TTCCCGGCGCAGGAGAAAGATCCGGTGCGCAATCAGCTGGCGGGAAGTCTGCGTGCGGTACTGGCGCAAAAACTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377