Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RIK66_RS04100 Genome accession   NZ_CP134635
Coordinates   664118..665143 (+) Length   341 a.a.
NCBI ID   WP_063925581.1    Uniprot ID   -
Organism   Enterobacter ludwigii strain 93A:C     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 659118..670143
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RIK66_RS04060 (RIK66_04060) - 659199..659525 (+) 327 WP_032678951.1 YggL family protein -
  RIK66_RS04065 (RIK66_04065) - 659582..660298 (+) 717 WP_020883689.1 DUF2884 domain-containing protein -
  RIK66_RS04070 (RIK66_04070) - 660360..660722 (-) 363 WP_020883690.1 endonuclease domain-containing protein -
  RIK66_RS04075 (RIK66_04075) hemW 660786..661928 (-) 1143 WP_063925582.1 radical SAM family heme chaperone HemW -
  RIK66_RS04080 (RIK66_04080) - 661921..662514 (-) 594 WP_014171572.1 XTP/dITP diphosphatase -
  RIK66_RS04085 (RIK66_04085) yggU 662518..662814 (-) 297 WP_014885193.1 DUF167 family protein YggU -
  RIK66_RS04090 (RIK66_04090) - 662811..663377 (-) 567 WP_014171570.1 YggT family protein -
  RIK66_RS04095 (RIK66_04095) - 663399..664100 (-) 702 WP_025203259.1 YggS family pyridoxal phosphate-dependent enzyme -
  RIK66_RS04100 (RIK66_04100) pilT 664118..665143 (+) 1026 WP_063925581.1 type IV pilus twitching motility protein PilT Machinery gene
  RIK66_RS04105 (RIK66_04105) ruvX 665109..665525 (-) 417 WP_014171567.1 Holliday junction resolvase RuvX -
  RIK66_RS04110 (RIK66_04110) - 665525..666085 (-) 561 WP_020883695.1 YqgE/AlgH family protein -
  RIK66_RS04115 (RIK66_04115) gshB 666172..667119 (-) 948 WP_020883696.1 glutathione synthase -
  RIK66_RS04120 (RIK66_04120) rsmE 667159..667890 (-) 732 WP_020883697.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RIK66_RS04125 (RIK66_04125) endA 667943..668650 (-) 708 WP_020883698.1 deoxyribonuclease I -
  RIK66_RS04130 (RIK66_04130) - 668745..669242 (-) 498 WP_032670169.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37128.15 Da        Isoelectric Point: 6.2326

>NTDB_id=882490 RIK66_RS04100 WP_063925581.1 664118..665143(+) (pilT) [Enterobacter ludwigii strain 93A:C]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPEVDTLLKAWLNDEQQGTWWANGQVDFAVTLAGNQRLRG
SAFKQTSGVSMTLRLLPGVCPQLSALGVPRVVPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FLYQSERCLIQQREIGQHSPSFADALRGALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDAQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGHQAGMQNFDQSLAER
RAQGQLQAPALNNPVRNSFRE

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=882490 RIK66_RS04100 WP_063925581.1 664118..665143(+) (pilT) [Enterobacter ludwigii strain 93A:C]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGATCAGGTCGCCTTGAACCCGCACCGTTTCCGCCGCCCGAAGTCGATACGCTATTAAAAGCGTGGCTCAACG
ATGAACAGCAGGGAACGTGGTGGGCGAACGGGCAGGTGGATTTTGCCGTCACCCTGGCGGGCAACCAGCGCCTGCGCGGA
AGCGCGTTTAAACAGACAAGCGGCGTCTCAATGACGCTGCGGCTGTTGCCCGGGGTCTGCCCGCAACTCTCCGCGTTGGG
GGTGCCCCGGGTTGTTCCTGAGCTGTTATCCAGTGACAACGGGTTGATCCTGGTCACCGGGGCAACCGGCAGCGGTAAGT
CCACTACGCTGGCAGCGATGGTAGATTTTCTTAATCACCATACTGACGGGCATATTCTGACGCTCGAAGACCCGGTGGAG
TTTCTATACCAGAGCGAACGGTGCCTGATCCAGCAGCGCGAGATTGGCCAACACAGCCCGTCATTTGCCGACGCGCTGCG
CGGGGCGTTACGCCAGGATCCTGATGTCATACTGCTGGGTGAGCTGCGCGACAGTGAAACAATCCGCCTCGCGCTGACGG
CGGCGGAAACCGGACATCTGGTGCTCGCGACACTGCATACCCGCGGGGCAGCGCAGGCGATTGAGCGGCTGGTCGATACG
TTTCCGGCGCAGGAGAAAGATCCGGTGCGCAATCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTCCTCCC
CGATGCGCAGGGCGGGCGCGTGGCGCTTTATGAGCTGCTGGTGAATACTCCGGCGGCGGCGAATCTCATTCGCGAAGGAA
AAACGTGGCAATTGCCCGGTATTATTCAAACCGGCCACCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCTGAAAGA
CGGGCGCAGGGGCAACTCCAGGCCCCGGCGCTCAATAACCCTGTTCGAAATAGCTTTCGAGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.112

99.12

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.112

99.12

0.487
  pilT Neisseria meningitidis 8013

48.048

97.654

0.469
  pilT Neisseria gonorrhoeae MS11

46.491

100

0.466
  pilT Legionella pneumophila strain ERS1305867

48.758

94.428

0.46
  pilT Legionella pneumophila strain Lp02

48.758

94.428

0.46
  pilT Acinetobacter baylyi ADP1

46.988

97.361

0.457
  pilT Pseudomonas stutzeri DSM 10701

47.706

95.894

0.457
  pilT Acinetobacter baumannii D1279779

47.401

95.894

0.455
  pilT Pseudomonas aeruginosa PAK

47.401

95.894

0.455
  pilT Acinetobacter baumannii strain A118

47.401

95.894

0.455
  pilT Acinetobacter nosocomialis M2

47.812

93.842

0.449
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.72

94.428

0.422
  pilU Pseudomonas stutzeri DSM 10701

40.303

96.774

0.39
  pilU Vibrio cholerae strain A1552

39.13

94.428

0.369