Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RIF24_RS11685 Genome accession   NZ_CP134083
Coordinates   2232392..2233438 (-) Length   348 a.a.
NCBI ID   WP_214771042.1    Uniprot ID   -
Organism   Exiguobacterium acetylicum strain TC1-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2227392..2238438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RIF24_RS11665 (RIF24_11655) - 2227968..2229707 (-) 1740 WP_410501602.1 fimbrial assembly protein -
  RIF24_RS11670 (RIF24_11660) - 2229726..2230481 (-) 756 WP_410501603.1 prepilin peptidase -
  RIF24_RS11675 (RIF24_11665) - 2230541..2231032 (-) 492 WP_410501604.1 type II secretion system protein -
  RIF24_RS11680 (RIF24_11670) - 2231170..2232387 (-) 1218 WP_410501605.1 type II secretion system F family protein -
  RIF24_RS11685 (RIF24_11675) pilT 2232392..2233438 (-) 1047 WP_214771042.1 type IV pilus twitching motility protein PilT Machinery gene
  RIF24_RS11690 (RIF24_11680) - 2233435..2235099 (-) 1665 WP_410501606.1 GspE/PulE family protein -
  RIF24_RS11695 (RIF24_11685) - 2235108..2235422 (-) 315 WP_029342268.1 hypothetical protein -
  RIF24_RS11700 (RIF24_11690) - 2235438..2236805 (-) 1368 WP_410501607.1 G5 domain-containing protein -
  RIF24_RS11705 (RIF24_11695) - 2236995..2237522 (+) 528 WP_410501608.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RIF24_RS11710 (RIF24_11700) - 2237485..2238105 (+) 621 WP_410501609.1 PilW family protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38155.90 Da        Isoelectric Point: 7.1317

>NTDB_id=878781 RIF24_RS11685 WP_214771042.1 2232392..2233438(-) (pilT) [Exiguobacterium acetylicum strain TC1-3]
MNSTMERAQIEDILRSSKERGASDVHLTAGSPPVFRINGTLTPFGTEKMKPIIIDGFVQSLITEEMYLQLKQDRDIDFSF
GVTGVARYRVNCFYQRGALSMAFRTIPTEVPTLEQLALPPVLKRFLTKPHGLILVTGPTGSGKSTTLAAMINEINQTMYK
RIITLEDPIEYLHSHHKSLIDQREIGIDAPRFATGLRSALRQDPDVILLGEMRDLETIATAVTAAETGHLVFATVHTSTA
ASTVSRIIDVFPSEQQDQIRAQLANVLAGVVSQRLMPRSDGTGRVAALEIMVDTPAVSNLIRTGKEYQIQSVLQTGKQYG
MQTMQMALQDLVSRGLIPASAASPYISG

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=878781 RIF24_RS11685 WP_214771042.1 2232392..2233438(-) (pilT) [Exiguobacterium acetylicum strain TC1-3]
ATGAACAGTACGATGGAACGGGCACAAATCGAGGACATTTTGCGCTCCTCAAAAGAACGAGGGGCGTCAGATGTTCATTT
AACGGCGGGATCACCACCTGTCTTTCGGATTAACGGGACGTTGACACCGTTTGGAACAGAGAAGATGAAACCGATCATCA
TTGACGGGTTTGTCCAATCATTGATTACGGAAGAGATGTATCTACAGCTGAAGCAGGACCGGGACATCGACTTTTCGTTC
GGTGTGACCGGTGTCGCTCGGTACCGCGTCAACTGTTTTTACCAACGCGGAGCTTTGTCGATGGCGTTTCGAACGATTCC
GACAGAAGTTCCGACACTCGAACAACTAGCACTTCCGCCTGTCTTAAAACGATTCTTAACGAAGCCACACGGCTTGATTC
TCGTCACAGGACCGACGGGTTCCGGGAAGTCTACGACGCTTGCAGCGATGATCAATGAAATCAATCAGACGATGTATAAA
CGGATCATTACGCTTGAGGATCCGATTGAGTACTTACATAGTCATCATAAAAGTCTTATCGATCAGCGGGAAATTGGGAT
CGATGCGCCACGCTTTGCAACCGGATTACGTTCAGCACTCCGGCAAGATCCAGATGTCATTTTGCTCGGGGAGATGCGAG
ATCTTGAAACGATTGCAACAGCCGTGACGGCAGCGGAGACGGGTCACCTCGTCTTTGCAACGGTCCATACATCAACGGCT
GCCTCGACGGTTAGTCGGATCATTGATGTTTTCCCGTCGGAGCAACAAGACCAGATTCGGGCTCAACTGGCGAACGTTCT
TGCGGGCGTCGTCTCGCAACGCTTAATGCCACGTTCGGATGGGACAGGACGCGTTGCAGCACTCGAAATCATGGTCGATA
CACCCGCTGTCTCGAACTTGATTCGGACCGGTAAGGAATATCAGATTCAATCCGTCCTGCAGACGGGTAAACAATACGGC
ATGCAGACGATGCAGATGGCATTGCAGGATCTCGTCAGTCGGGGATTGATTCCGGCTTCTGCTGCATCACCGTACATATC
GGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.524

94.253

0.486
  pilT Neisseria meningitidis 8013

50

95.977

0.48
  pilT Neisseria gonorrhoeae MS11

50

95.977

0.48
  pilT Acinetobacter nosocomialis M2

48.949

95.69

0.468
  pilT Pseudomonas aeruginosa PAK

48.949

95.69

0.468
  pilT Acinetobacter baylyi ADP1

47.941

97.701

0.468
  pilT Acinetobacter baumannii D1279779

48.649

95.69

0.466
  pilT Acinetobacter baumannii strain A118

48.649

95.69

0.466
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.091

94.828

0.466
  pilT Vibrio cholerae strain A1552

49.091

94.828

0.466
  pilT Pseudomonas stutzeri DSM 10701

47.748

95.69

0.457
  pilT Legionella pneumophila strain Lp02

46.246

95.69

0.443
  pilT Legionella pneumophila strain ERS1305867

46.246

95.69

0.443
  pilU Pseudomonas stutzeri DSM 10701

40.426

94.54

0.382
  pilU Acinetobacter baylyi ADP1

40.06

95.402

0.382