Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RHN82_RS13610 Genome accession   NZ_CP133860
Coordinates   2845126..2846106 (+) Length   326 a.a.
NCBI ID   WP_032619064.1    Uniprot ID   A0AA37AL66
Organism   Enterobacter cloacae complex sp. EB5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2840126..2851106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHN82_RS13570 (RHN82_13570) - 2840212..2840538 (+) 327 WP_003862421.1 YggL family protein -
  RHN82_RS13575 (RHN82_13575) - 2840596..2841312 (+) 717 WP_003862419.1 DUF2884 domain-containing protein -
  RHN82_RS13580 (RHN82_13580) - 2841393..2841731 (-) 339 WP_047050751.1 endonuclease domain-containing protein -
  RHN82_RS13585 (RHN82_13585) hemW 2841788..2842936 (-) 1149 WP_047050752.1 radical SAM family heme chaperone HemW -
  RHN82_RS13590 (RHN82_13590) - 2842929..2843522 (-) 594 WP_015571839.1 XTP/dITP diphosphatase -
  RHN82_RS13595 (RHN82_13595) yggU 2843526..2843822 (-) 297 WP_023304404.1 DUF167 family protein YggU -
  RHN82_RS13600 (RHN82_13600) - 2843819..2844385 (-) 567 WP_047050754.1 YggT family protein -
  RHN82_RS13605 (RHN82_13605) - 2844407..2845108 (-) 702 WP_047050755.1 YggS family pyridoxal phosphate-dependent enzyme -
  RHN82_RS13610 (RHN82_13610) pilT 2845126..2846106 (+) 981 WP_032619064.1 type IV pilus twitching motility protein PilT Machinery gene
  RHN82_RS13615 (RHN82_13615) ruvX 2846117..2846533 (-) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  RHN82_RS13620 (RHN82_13620) - 2846533..2847093 (-) 561 WP_006811924.1 YqgE/AlgH family protein -
  RHN82_RS13625 (RHN82_13625) gshB 2847168..2848115 (-) 948 WP_047050758.1 glutathione synthase -
  RHN82_RS13630 (RHN82_13630) rsmE 2848147..2848878 (-) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RHN82_RS13635 (RHN82_13635) endA 2848930..2849637 (-) 708 WP_047050759.1 deoxyribonuclease I -
  RHN82_RS13640 (RHN82_13640) - 2849732..2850229 (-) 498 WP_017692693.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35706.68 Da        Isoelectric Point: 6.4131

>NTDB_id=877263 RHN82_RS13610 WP_032619064.1 2845126..2846106(+) (pilT) [Enterobacter cloacae complex sp. EB5]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=877263 RHN82_RS13610 WP_032619064.1 2845126..2846106(+) (pilT) [Enterobacter cloacae complex sp. EB5]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCATTATTAAAAGCGTGGCTCAACG
ATGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAACTGCTGTCTAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGAAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCATCAGACAGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTACG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCATACGCGCGGCGCGGCGCAGGCGATTGAGCGCCTGGTCGATACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTGCTGGTGAATACGTCGGCGGCGGCCAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374