Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RF679_RS14875 Genome accession   NZ_CP133720
Coordinates   3566219..3567355 (+) Length   378 a.a.
NCBI ID   WP_309481413.1    Uniprot ID   -
Organism   Undibacterium cyanobacteriorum strain 20NA77.5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3561219..3572355
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RF679_RS14850 (RF679_14850) - 3561468..3562412 (+) 945 WP_309481408.1 hydrogen peroxide-inducible genes activator -
  RF679_RS14855 (RF679_14855) ubiA 3562509..3563375 (+) 867 WP_309481409.1 4-hydroxybenzoate octaprenyltransferase -
  RF679_RS14860 (RF679_14860) proC 3563384..3564223 (-) 840 WP_309481410.1 pyrroline-5-carboxylate reductase -
  RF679_RS14865 (RF679_14865) - 3564213..3564944 (-) 732 WP_309481411.1 YggS family pyridoxal phosphate-dependent enzyme -
  RF679_RS14870 (RF679_14870) pilT 3565133..3566176 (+) 1044 WP_309481412.1 type IV pilus twitching motility protein PilT Machinery gene
  RF679_RS14875 (RF679_14875) pilU 3566219..3567355 (+) 1137 WP_309481413.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RF679_RS14880 (RF679_14880) - 3567352..3567846 (+) 495 WP_309481414.1 glutathione peroxidase -
  RF679_RS14885 (RF679_14885) - 3568292..3568660 (+) 369 WP_309481415.1 hypothetical protein -
  RF679_RS14895 (RF679_14895) - 3568826..3569557 (+) 732 WP_373921691.1 ZIP family metal transporter -
  RF679_RS14900 (RF679_14900) - 3569573..3570454 (-) 882 WP_309481417.1 sulfurtransferase -
  RF679_RS14905 (RF679_14905) - 3570523..3571461 (-) 939 WP_309481418.1 DMT family transporter -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41970.48 Da        Isoelectric Point: 6.5769

>NTDB_id=876105 RF679_RS14875 WP_309481413.1 3566219..3567355(+) (pilU) [Undibacterium cyanobacteriorum strain 20NA77.5]
MERDQATKFMNDLLKLMLSKNGSDLFITADFPPAFKIDGKVTPVSNQVLTSAHTIDLARAIMNDRQASEFESTKECNFAI
NPPGLGRFRVSAFLQQGKVGMVLRTITSAIPTFEELRLPPQLKDIAMTKRGLVIMVGATGSGKSTTLAAMVGHRNANSYG
HIITIEDPIEYVHPHGNCIITQREVGIDTDDWEIALKNTLRQAPDVIQIGEIRSRETMDLAIAFAETGHLCLATLHANSA
NQALDRMINFFPEERRQQLLMDLSLNLKAVISQRLVPMRLQKGRVAAIEIMINSPLISDLIFKGDVHEIKEIMKKSEELG
MMTFDKALFDLHEADLISYEDALRNADSVNELRLAIKLKGKDASNRDLSAGTHHLGIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=876105 RF679_RS14875 WP_309481413.1 3566219..3567355(+) (pilU) [Undibacterium cyanobacteriorum strain 20NA77.5]
ATGGAACGCGATCAGGCCACCAAGTTTATGAACGATTTGCTCAAGCTGATGTTGAGCAAAAATGGTTCCGATCTTTTTAT
CACGGCCGACTTCCCTCCCGCCTTTAAAATCGATGGCAAAGTGACGCCAGTATCCAATCAAGTACTAACCTCAGCCCACA
CCATCGATTTGGCGCGGGCCATCATGAACGACCGTCAAGCCTCTGAATTTGAATCAACTAAGGAATGTAACTTTGCGATT
AATCCACCGGGCTTAGGGCGTTTCCGTGTCTCCGCTTTCCTACAACAAGGTAAAGTCGGTATGGTTCTTCGTACCATTAC
TTCTGCGATTCCGACCTTTGAAGAACTACGTTTGCCGCCACAACTCAAAGATATTGCCATGACCAAGCGCGGTCTGGTGA
TCATGGTCGGTGCGACCGGTTCTGGTAAATCCACCACGCTCGCTGCGATGGTAGGCCACCGCAATGCCAACAGCTACGGC
CACATCATCACCATTGAAGATCCAATTGAATATGTCCACCCACATGGCAATTGCATCATCACCCAACGTGAAGTCGGTAT
CGATACCGATGATTGGGAAATCGCATTGAAAAACACTTTGCGTCAAGCCCCAGATGTCATTCAAATCGGTGAGATTCGCT
CACGCGAAACCATGGATTTAGCGATTGCCTTCGCTGAAACTGGTCACTTGTGCTTAGCCACCTTGCACGCCAACAGCGCC
AACCAAGCACTTGACCGTATGATCAACTTCTTCCCAGAAGAGCGACGTCAACAGCTATTAATGGATTTATCACTCAACTT
AAAGGCGGTCATTTCTCAGCGTTTGGTTCCAATGCGTCTTCAAAAAGGCCGCGTTGCCGCCATCGAAATTATGATCAACA
GCCCATTGATCTCGGACCTCATTTTCAAAGGTGACGTCCATGAGATCAAAGAAATCATGAAGAAATCGGAAGAGCTCGGC
ATGATGACCTTTGACAAAGCTTTGTTCGATCTGCACGAAGCCGATCTCATCTCTTACGAAGATGCCTTGCGTAATGCCGA
CTCTGTCAATGAATTGCGCCTTGCGATTAAGTTGAAAGGCAAGGACGCCAGCAATCGTGATTTGTCGGCCGGCACCCATC
ATTTGGGGATTGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.873

91.534

0.585
  pilU Acinetobacter baylyi ADP1

61.143

92.593

0.566
  pilU Vibrio cholerae strain A1552

54.958

93.386

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.516

91.799

0.399
  pilT Pseudomonas aeruginosa PAK

42.73

89.153

0.381
  pilT Acinetobacter baylyi ADP1

42.183

89.683

0.378
  pilT Acinetobacter nosocomialis M2

42.643

88.095

0.376
  pilT Acinetobacter baumannii strain A118

42.643

88.095

0.376
  pilT Acinetobacter baumannii D1279779

42.643

88.095

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.84

89.153

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.444

83.333

0.37
  pilT Vibrio cholerae strain A1552

44.444

83.333

0.37
  pilT Legionella pneumophila strain Lp02

41.39

87.566

0.362
  pilT Legionella pneumophila strain ERS1305867

41.39

87.566

0.362