Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RCR42_RS18530 Genome accession   NZ_CP133336
Coordinates   3838298..3839278 (-) Length   326 a.a.
NCBI ID   WP_265186636.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain CRECL423     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3833298..3844278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCR42_RS18500 (RCR42_18500) - 3834175..3834672 (+) 498 WP_032621896.1 SprT family zinc-dependent metalloprotease -
  RCR42_RS18505 (RCR42_18505) endA 3834767..3835474 (+) 708 WP_023306912.1 deoxyribonuclease I -
  RCR42_RS18510 (RCR42_18510) rsmE 3835526..3836257 (+) 732 WP_003860033.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RCR42_RS18515 (RCR42_18515) gshB 3836289..3837236 (+) 948 WP_199126774.1 glutathione synthase -
  RCR42_RS18520 (RCR42_18520) - 3837311..3837871 (+) 561 WP_022649353.1 YqgE/AlgH family protein -
  RCR42_RS18525 (RCR42_18525) ruvX 3837871..3838287 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  RCR42_RS18530 (RCR42_18530) pilT 3838298..3839278 (-) 981 WP_265186636.1 type IV pilus twitching motility protein PilT Machinery gene
  RCR42_RS18535 (RCR42_18535) - 3839296..3839997 (+) 702 WP_199126772.1 YggS family pyridoxal phosphate-dependent enzyme -
  RCR42_RS18540 (RCR42_18540) - 3840019..3840585 (+) 567 WP_003860024.1 YggT family protein -
  RCR42_RS18545 (RCR42_18545) yggU 3840582..3840878 (+) 297 WP_022649356.1 DUF167 family protein YggU -
  RCR42_RS18550 (RCR42_18550) - 3840882..3841475 (+) 594 WP_022649357.1 XTP/dITP diphosphatase -
  RCR42_RS18555 (RCR42_18555) hemW 3841468..3842616 (+) 1149 WP_063146830.1 radical SAM family heme chaperone HemW -
  RCR42_RS18560 (RCR42_18560) - 3842673..3843011 (+) 339 WP_023298571.1 endonuclease domain-containing protein -
  RCR42_RS18565 (RCR42_18565) - 3843095..3843811 (-) 717 WP_022649360.1 DUF2884 domain-containing protein -
  RCR42_RS18570 (RCR42_18570) - 3843869..3844195 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35738.69 Da        Isoelectric Point: 6.5004

>NTDB_id=873511 RCR42_RS18530 WP_265186636.1 3838298..3839278(-) (pilT) [Enterobacter hormaechei strain CRECL423]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQFSALGVPRAIPELLSNDNGLILVTGTTGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIRQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=873511 RCR42_RS18530 WP_265186636.1 3838298..3839278(-) (pilT) [Enterobacter hormaechei strain CRECL423]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCCGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAGCAGCAGGGTGCCTGGTGGGCAAACGGGCAGGTAGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTACGCCTGTTGCCGCTGACGTGTCCGCAGTTCTCTGCGTTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAACGGCCTGATTCTGGTCACCGGCACCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTTCTCAATCACCATACGGACGGGCACATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTCATCCGGCAGCGGGAGATAGGCCAGCATAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGTGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACAG
CGGCTGAGACTGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGATACC
TTCCCGGCGCAGGAGAAAGATCCGGTTCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTACTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38