Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RAN89_RS01245 Genome accession   NZ_CP132507
Coordinates   271362..272498 (+) Length   378 a.a.
NCBI ID   WP_087495403.1    Uniprot ID   A0A1Y0NAF6
Organism   Rhodoferax mekongensis strain TBRC 17307     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 266362..277498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAN89_RS01225 (RAN89_01225) - 267781..268251 (+) 471 WP_087495399.1 Lrp/AsnC ligand binding domain-containing protein -
  RAN89_RS01230 (RAN89_01230) - 268294..269421 (+) 1128 WP_313867878.1 ABC transporter substrate-binding protein -
  RAN89_RS01235 (RAN89_01235) - 269418..270116 (-) 699 WP_313867879.1 YggS family pyridoxal phosphate-dependent enzyme -
  RAN89_RS01240 (RAN89_01240) pilT 270163..271206 (+) 1044 WP_313867880.1 type IV pilus twitching motility protein PilT Machinery gene
  RAN89_RS01245 (RAN89_01245) pilU 271362..272498 (+) 1137 WP_087495403.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RAN89_RS01250 (RAN89_01250) - 272502..273428 (+) 927 WP_313867881.1 NAD(P)-dependent oxidoreductase -
  RAN89_RS01255 (RAN89_01255) - 273479..274108 (-) 630 WP_313867882.1 BON domain-containing protein -
  RAN89_RS01260 (RAN89_01260) - 274105..274704 (-) 600 WP_313867883.1 phosphoheptose isomerase -
  RAN89_RS01265 (RAN89_01265) - 274763..275149 (-) 387 WP_313867884.1 YraN family protein -
  RAN89_RS01270 (RAN89_01270) rsmI 275150..276073 (+) 924 WP_313867885.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  RAN89_RS01275 (RAN89_01275) - 276070..276708 (-) 639 WP_313867886.1 2-dehydro-3-deoxy-6-phosphogalactonate aldolase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41936.22 Da        Isoelectric Point: 6.9719

>NTDB_id=869303 RAN89_RS01245 WP_087495403.1 271362..272498(+) (pilU) [Rhodoferax mekongensis strain TBRC 17307]
MERDQATKFINDLLKLMVSRNGSDLFITAEFPPAIKVDGKVTKVSPQPLNSAHTMALARSIMNDKQIAEFERTKESNFAI
APATIGRFRVNAFIQQGKVGMVLRVIPQVLPTIDGLGVPQVLKDIVQSKRGLCILVGATGSGKSTTLAAMVDWRNENSYG
HIITIEDPVEFVHAHKNCVITQREVGLDTDSWDAALKNTLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANSA
NQALDRVINFFPEERRSQLLMDLSLNLRAMVSQRLIPKQDGKGRVAAVEVMINSPLISDLIFKGEVSEVKEIMKKSRQAG
MQTFDQALFDLYEGHAITYEDALRNADSLNDLRLQIKLNSHRGKSQDLSAGTENFAIM

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=869303 RAN89_RS01245 WP_087495403.1 271362..272498(+) (pilU) [Rhodoferax mekongensis strain TBRC 17307]
ATGGAACGCGATCAAGCCACGAAATTTATCAACGATCTGCTCAAGTTGATGGTCAGCCGCAATGGCAGCGACTTGTTCAT
CACCGCGGAGTTTCCGCCCGCCATCAAGGTAGACGGTAAAGTCACCAAGGTGTCGCCACAGCCGCTGAACTCGGCCCACA
CCATGGCGCTGGCGCGTTCCATCATGAACGACAAGCAGATTGCCGAGTTCGAGCGCACCAAGGAGAGTAATTTCGCGATT
GCACCGGCCACCATCGGCCGGTTCCGGGTCAACGCTTTTATCCAGCAGGGCAAGGTCGGGATGGTGCTGCGCGTGATTCC
GCAAGTTCTGCCCACCATCGACGGTCTGGGTGTGCCCCAGGTGCTCAAAGACATTGTGCAAAGCAAGCGCGGACTGTGCA
TTCTGGTGGGCGCCACCGGCTCCGGCAAATCCACCACGCTGGCTGCCATGGTGGACTGGCGCAATGAGAACTCCTACGGC
CACATCATCACTATCGAAGACCCGGTGGAGTTTGTGCATGCTCACAAGAACTGCGTAATCACCCAGCGCGAAGTCGGGTT
GGACACGGACAGCTGGGACGCGGCTTTGAAGAACACCTTGCGCCAGGCGCCGGATGTGATTCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAACACGCCATCGCGTTTTCTGAAACCGGTCACCTGTGCCTGGCCACCCTGCACGCCAACAGTGCC
AACCAGGCGCTGGACCGCGTGATCAACTTCTTCCCTGAAGAGCGGCGCAGCCAGTTGCTGATGGACTTGTCGCTCAACCT
GCGCGCCATGGTGTCCCAGCGCCTGATCCCCAAGCAGGACGGCAAGGGCCGAGTGGCGGCGGTGGAAGTGATGATCAACT
CCCCGCTGATCTCTGACCTCATCTTCAAGGGTGAAGTCTCGGAAGTGAAAGAGATCATGAAGAAGAGCCGCCAGGCCGGC
ATGCAGACCTTCGATCAGGCCCTCTTCGATCTGTACGAGGGCCACGCCATCACCTACGAAGACGCACTGCGCAACGCCGA
CTCGCTCAACGACTTGCGTCTCCAAATCAAGCTCAACAGCCACCGTGGCAAGTCGCAAGACCTGTCGGCCGGCACTGAGA
ACTTCGCCATCATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(36-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y0NAF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.644

92.063

0.577
  pilU Acinetobacter baylyi ADP1

57.103

94.974

0.542
  pilU Vibrio cholerae strain A1552

52.874

92.063

0.487
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

89.153

0.399
  pilT Pseudomonas aeruginosa PAK

43.917

89.153

0.392
  pilT Acinetobacter nosocomialis M2

44.444

88.095

0.392
  pilT Acinetobacter baumannii D1279779

45.455

84.392

0.384
  pilT Acinetobacter baumannii strain A118

45.455

84.392

0.384
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Legionella pneumophila strain Lp02

42.943

88.095

0.378
  pilT Legionella pneumophila strain ERS1305867

42.943

88.095

0.378
  pilT Acinetobacter baylyi ADP1

43.789

85.185

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.533

83.862

0.365
  pilT Vibrio cholerae strain A1552

43.533

83.862

0.365