Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RAN89_RS01240 Genome accession   NZ_CP132507
Coordinates   270163..271206 (+) Length   347 a.a.
NCBI ID   WP_313867880.1    Uniprot ID   -
Organism   Rhodoferax mekongensis strain TBRC 17307     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 265163..276206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAN89_RS01220 (RAN89_01220) - 265807..267771 (+) 1965 WP_313867877.1 methyl-accepting chemotaxis protein -
  RAN89_RS01225 (RAN89_01225) - 267781..268251 (+) 471 WP_087495399.1 Lrp/AsnC ligand binding domain-containing protein -
  RAN89_RS01230 (RAN89_01230) - 268294..269421 (+) 1128 WP_313867878.1 ABC transporter substrate-binding protein -
  RAN89_RS01235 (RAN89_01235) - 269418..270116 (-) 699 WP_313867879.1 YggS family pyridoxal phosphate-dependent enzyme -
  RAN89_RS01240 (RAN89_01240) pilT 270163..271206 (+) 1044 WP_313867880.1 type IV pilus twitching motility protein PilT Machinery gene
  RAN89_RS01245 (RAN89_01245) pilU 271362..272498 (+) 1137 WP_087495403.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RAN89_RS01250 (RAN89_01250) - 272502..273428 (+) 927 WP_313867881.1 NAD(P)-dependent oxidoreductase -
  RAN89_RS01255 (RAN89_01255) - 273479..274108 (-) 630 WP_313867882.1 BON domain-containing protein -
  RAN89_RS01260 (RAN89_01260) - 274105..274704 (-) 600 WP_313867883.1 phosphoheptose isomerase -
  RAN89_RS01265 (RAN89_01265) - 274763..275149 (-) 387 WP_313867884.1 YraN family protein -
  RAN89_RS01270 (RAN89_01270) rsmI 275150..276073 (+) 924 WP_313867885.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38229.02 Da        Isoelectric Point: 6.8887

>NTDB_id=869302 RAN89_RS01240 WP_313867880.1 270163..271206(+) (pilT) [Rhodoferax mekongensis strain TBRC 17307]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVEPLDHKQVHAMVYDIMNDTQRKMYEEFLEIDFSFEIDGLAR
FRVNAFNHNRGAGAVFRTIPSKILSLEQLNAPRIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEFGHILTVED
PIEFVHESKKCLVNQREVGPHTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVAQMYSAIQTGNSVGMQTLDQN
LTELVKRNVISAAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=869302 RAN89_RS01240 WP_313867880.1 270163..271206(+) (pilT) [Rhodoferax mekongensis strain TBRC 17307]
GTGGACATTACCCAGCTGCTCGCATTTAGCGTCAAAAACAAAGCGTCTGACTTGCACCTCTCGGCAGGACTGCCGCCCAT
GATCCGGGTGCACGGCGATGTGCGCCGCATCAACGTCGAACCCTTGGACCACAAGCAGGTTCACGCCATGGTGTATGACA
TCATGAACGACACCCAGCGCAAGATGTATGAAGAGTTTCTGGAAATCGACTTCTCGTTCGAGATTGATGGCCTGGCCCGC
TTCCGCGTCAACGCCTTCAACCACAACCGCGGCGCAGGCGCCGTGTTCCGGACGATTCCGAGCAAGATCCTCTCGCTGGA
ACAGCTCAACGCCCCCCGCATCTTTGCAGACCTGGCCCTGAAGCCCCGCGGTCTGGTGCTGGTGACCGGCCCTACCGGTT
CCGGCAAGTCCACGACCCTGGCGGCCATGGTGAACTACCTGAACGAAACCGAGTTCGGCCACATCCTGACGGTGGAAGAC
CCGATCGAATTCGTGCACGAATCCAAGAAGTGCCTGGTCAACCAGCGTGAAGTCGGCCCCCATACCCTGAGCTTTGCCGC
CGCCCTGAAGTCCGCCCTGCGGGAAGACCCGGACGCGATTTTGGTGGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CCATGACAGCGGCGGAAACCGGCCACTTGGTGTTCGGCACATTGCACACCTCCAGTGCTGCCAAAACCATTGACCGGATC
ATCGACGTGTTCCCGGCCGAAGAAAAAGAAATGGTGCGGGCCATGTTGTCCGAGTCGCTGCAAGCCGTAATCTCGCAAAC
CCTGTGCAAAACCAAAGACGGCCAGGGCCGCGTGGCAGCCCACGAGATCATGCTGGGCACCAGCGCGATCCGTAACCTGA
TCCGCGAAGCCAAGGTCGCGCAGATGTACTCCGCCATCCAGACCGGTAACAGCGTAGGCATGCAAACCCTGGACCAGAAC
CTCACCGAGCTAGTTAAGCGCAACGTAATCAGCGCGGCCGAAGCACGCAGCAAAGCCAAGATTCCTGAGAACTTCCCAGG
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.971

99.424

0.775
  pilT Pseudomonas stutzeri DSM 10701

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

74.336

97.695

0.726
  pilT Legionella pneumophila strain ERS1305867

74.336

97.695

0.726
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.155

98.271

0.66
  pilT Vibrio cholerae strain A1552

67.155

98.271

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Vibrio cholerae strain A1552

43.881

96.542

0.424
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406