Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q9293_RS12865 Genome accession   NZ_CP132333
Coordinates   2921146..2922273 (-) Length   375 a.a.
NCBI ID   WP_372342212.1    Uniprot ID   -
Organism   Geothrix sp. PMB-07     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2912212..2921075 2921146..2922273 flank 71


Gene organization within MGE regions


Location: 2912212..2922273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9293_RS12830 (Q9293_12830) - 2912212..2912736 (+) 525 WP_306247096.1 type II secretion system protein -
  Q9293_RS12835 (Q9293_12835) - 2912980..2914036 (-) 1057 Protein_2538 IS3 family transposase -
  Q9293_RS12840 (Q9293_12840) - 2914342..2916123 (-) 1782 WP_306247098.1 hypothetical protein -
  Q9293_RS12845 (Q9293_12845) - 2916414..2918441 (-) 2028 WP_306247100.1 glycosyltransferase family 39 protein -
  Q9293_RS12850 (Q9293_12850) - 2918438..2919211 (-) 774 WP_306247102.1 glycosyltransferase family 2 protein -
  Q9293_RS12855 (Q9293_12855) - 2919397..2919828 (-) 432 WP_306247104.1 type IV pilin protein -
  Q9293_RS12860 (Q9293_12860) - 2919873..2921075 (-) 1203 WP_306247106.1 type II secretion system F family protein -
  Q9293_RS12865 (Q9293_12865) pilT 2921146..2922273 (-) 1128 WP_372342212.1 type IV pilus twitching motility protein PilT Machinery gene

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41417.25 Da        Isoelectric Point: 7.2866

>NTDB_id=868180 Q9293_RS12865 WP_372342212.1 2921146..2922273(-) (pilT) [Geothrix sp. PMB-07]
MVEYGGTDLHITTETAPQIRIDGRMVPLKLPPLDAAQTRWLCYGVMTDQQKHRLEEDLEVDFSFGLQGVARFRANVFNQR
GATAGVFRTIPEQIRSFDQLGLPPSIQALCDKPRGLVLVTGVTGSGKSTTLAAMVDKINAEEPLHILTIEDPVEYVHKHK
RALVNQREIHADTHSFKKALRSALRQDPDVVLVGELRDLETIESALTIAETGHLTFGTLHTNSTVQTINRIIDVFPSHQQ
PQVRAQLSLVLEGVVCQSLIPKASGKGRALALEIMIPNSAIRNLIREDKIHQIYGTMQAGQQKYGMQTFNQSLADLVMRK
EITQEQAFEYSSNTDELRELINRGVGLGSAGGRANTPAQPTSSILGGRNPNIKYT

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=868180 Q9293_RS12865 WP_372342212.1 2921146..2922273(-) (pilT) [Geothrix sp. PMB-07]
ATGGTGGAGTACGGGGGGACGGATCTGCACATCACCACGGAAACGGCCCCCCAGATTCGCATCGACGGCCGCATGGTGCC
GCTCAAGCTGCCTCCCCTGGATGCCGCCCAGACGCGCTGGCTCTGCTACGGCGTCATGACCGACCAGCAGAAACACCGCC
TGGAAGAGGATCTGGAAGTGGACTTCTCCTTCGGTCTCCAGGGCGTGGCCCGCTTCCGCGCCAATGTGTTCAACCAGCGG
GGTGCCACCGCCGGTGTGTTTCGCACGATTCCCGAACAGATCCGCAGCTTCGACCAGCTGGGCCTGCCGCCCTCCATCCA
GGCCCTCTGCGACAAGCCCCGAGGCCTGGTGCTGGTCACGGGGGTGACGGGGTCAGGCAAGTCCACCACCCTGGCGGCCA
TGGTGGACAAGATCAATGCAGAGGAACCGCTTCACATCCTCACCATTGAAGATCCGGTGGAATACGTCCACAAGCACAAG
CGGGCTCTGGTGAATCAGCGGGAGATCCACGCAGATACCCACAGCTTCAAGAAGGCCCTGCGATCGGCCCTTCGCCAGGA
TCCCGATGTGGTGCTGGTGGGTGAGCTGCGCGATCTGGAGACCATCGAATCAGCCCTGACGATCGCCGAAACTGGCCACC
TCACCTTCGGCACGCTGCACACCAACAGCACCGTGCAGACCATCAACCGCATCATCGACGTGTTCCCCAGCCACCAGCAA
CCTCAAGTCCGAGCCCAGCTTTCCCTGGTGCTGGAAGGGGTCGTCTGCCAAAGCCTCATTCCCAAGGCCAGTGGCAAAGG
CCGGGCTTTGGCCCTGGAAATCATGATCCCCAACTCGGCCATCCGGAACCTCATTCGCGAGGATAAGATCCACCAGATCT
ACGGCACCATGCAGGCCGGCCAACAAAAATACGGCATGCAGACCTTCAACCAGAGCCTCGCAGATCTGGTCATGCGCAAG
GAGATCACCCAAGAGCAGGCCTTCGAATACTCATCCAATACCGATGAGTTGCGTGAACTCATCAACCGGGGCGTTGGCCT
CGGATCCGCAGGGGGGCGAGCAAATACGCCCGCTCAACCCACCAGTTCCATCCTGGGTGGGCGAAATCCCAACATCAAAT
ACACCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(3-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

56.024

88.533

0.496
  pilT Legionella pneumophila strain Lp02

56.024

88.533

0.496
  pilT Pseudomonas aeruginosa PAK

54.462

86.667

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.167

96

0.472
  pilT Acinetobacter nosocomialis M2

53.988

86.933

0.469
  pilT Acinetobacter baumannii strain A118

53.988

86.933

0.469
  pilT Acinetobacter baumannii D1279779

53.988

86.933

0.469
  pilT Pseudomonas stutzeri DSM 10701

53.231

86.667

0.461
  pilT Acinetobacter baylyi ADP1

54.088

84.8

0.459
  pilT Neisseria meningitidis 8013

51.205

88.533

0.453
  pilT Neisseria gonorrhoeae MS11

50.904

88.533

0.451
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.83

84.8

0.448
  pilT Vibrio cholerae strain A1552

52.83

84.8

0.448
  pilU Pseudomonas stutzeri DSM 10701

41.57

91.733

0.381
  pilU Vibrio cholerae strain A1552

40.171

93.6

0.376