Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q3350_RS19020 Genome accession   NZ_CP130605
Coordinates   3944681..3945661 (+) Length   326 a.a.
NCBI ID   WP_265189614.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. xiangfangensis strain HD2649     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3939681..3950661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q3350_RS18980 (Q3350_18950) - 3939764..3940090 (+) 327 WP_023304407.1 YggL family protein -
  Q3350_RS18985 (Q3350_18955) - 3940148..3940864 (+) 717 WP_023304406.1 DUF2884 domain-containing protein -
  Q3350_RS18990 (Q3350_18960) - 3940948..3941286 (-) 339 WP_023304405.1 endonuclease domain-containing protein -
  Q3350_RS18995 (Q3350_18965) hemW 3941343..3942491 (-) 1149 WP_017382926.1 radical SAM family heme chaperone HemW -
  Q3350_RS19000 (Q3350_18970) - 3942484..3943077 (-) 594 WP_015571839.1 XTP/dITP diphosphatase -
  Q3350_RS19005 (Q3350_18975) yggU 3943081..3943377 (-) 297 WP_023304404.1 DUF167 family protein YggU -
  Q3350_RS19010 (Q3350_18980) - 3943374..3943940 (-) 567 WP_015571838.1 YggT family protein -
  Q3350_RS19015 (Q3350_18985) - 3943962..3944663 (-) 702 WP_023304403.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q3350_RS19020 (Q3350_18990) pilT 3944681..3945661 (+) 981 WP_265189614.1 type IV pilus twitching motility protein PilT Machinery gene
  Q3350_RS19025 (Q3350_18995) ruvX 3945671..3946087 (-) 417 WP_023304402.1 Holliday junction resolvase RuvX -
  Q3350_RS19030 (Q3350_19000) - 3946087..3946647 (-) 561 WP_006811924.1 YqgE/AlgH family protein -
  Q3350_RS19035 (Q3350_19005) gshB 3946722..3947669 (-) 948 WP_015571835.1 glutathione synthase -
  Q3350_RS19040 (Q3350_19010) rsmE 3947701..3948432 (-) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Q3350_RS19045 (Q3350_19015) endA 3948484..3949191 (-) 708 WP_003860034.1 deoxyribonuclease I -
  Q3350_RS19050 (Q3350_19020) - 3949286..3949783 (-) 498 WP_015571833.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35668.68 Da        Isoelectric Point: 6.3047

>NTDB_id=860547 Q3350_RS19020 WP_265189614.1 3944681..3945661(+) (pilT) [Enterobacter hormaechei subsp. xiangfangensis strain HD2649]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMLGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHVLTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=860547 Q3350_RS19020 WP_265189614.1 3944681..3945661(+) (pilT) [Enterobacter hormaechei subsp. xiangfangensis strain HD2649]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAAGCATTATTAAAAGCGTGGCTCAACG
ATGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCTCGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAACTGCTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCTATGGTCGATTTCCTCAATCACCAGACGGACGGGCATGTTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCACACGCGCGGCGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.034

99.08

0.426
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

37.578

98.773

0.371