Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LC603172_RS11750 Genome accession   NZ_AP024565
Coordinates   2349298..2350398 (-) Length   366 a.a.
NCBI ID   WP_061432558.1    Uniprot ID   A0A1V4BYN4
Organism   Microcystis aeruginosa strain NIES-88     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2344298..2355398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LC603172_RS28065 - 2344526..2344651 (-) 126 WP_260324559.1 hypothetical protein -
  LC603172_RS11730 (MAN88_22760) - 2344683..2347253 (+) 2571 WP_061432555.1 TIGR03960 family B12-binding radical SAM protein -
  LC603172_RS11735 (MAN88_22770) - 2347296..2347832 (-) 537 WP_061432556.1 hypothetical protein -
  LC603172_RS11740 (MAN88_22780) - 2347928..2349142 (-) 1215 WP_061432557.1 type II secretion system F family protein -
  LC603172_RS11745 - 2349075..2349284 (+) 210 WP_192846050.1 hypothetical protein -
  LC603172_RS11750 (MAN88_22790) pilT 2349298..2350398 (-) 1101 WP_061432558.1 type IV pilus twitching motility protein PilT Machinery gene
  LC603172_RS11755 (MAN88_22800) pilB/pilB1 2350529..2352544 (-) 2016 WP_061432559.1 GspE/PulE family protein Machinery gene
  LC603172_RS11760 (MAN88_22810) grpE 2352785..2353507 (+) 723 WP_061432560.1 nucleotide exchange factor GrpE -
  LC603172_RS11765 (MAN88_22820) - 2353539..2354258 (-) 720 WP_061432561.1 HAD-IA family hydrolase -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40334.54 Da        Isoelectric Point: 7.8147

>NTDB_id=86031 LC603172_RS11750 WP_061432558.1 2349298..2350398(-) (pilT) [Microcystis aeruginosa strain NIES-88]
MELMIEDLMEQLVEMGGSDMHIQSGAPVYFRISGKLSPINEESLTPQDCQKLIFSMLNNTQRKELEQNWELDCSYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEQLNLPRVVRELSERPRGLILVTGQTGSGKTTTLAAILDLINRTRSEHILTV
EDPIEYVFPNIKSLFHQRQKGEDTKSFANALRASLREDPDIILVGEMRDLETISLAITAAETGHLVFGTLHTSSAAGTVD
RIIDVFPANQQAQIRAMLSNSLLAVFSQNLVKKKNPKPGEFGRAMVQEIMVVTPAIANLIREGKAPQVYSAIQTGMKLGM
QTMEQGLANLVVRGVVSLEEAISKSGKPDELQRLIAGASANAKARI

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=86031 LC603172_RS11750 WP_061432558.1 2349298..2350398(-) (pilT) [Microcystis aeruginosa strain NIES-88]
ATGGAATTAATGATCGAAGATTTAATGGAACAATTGGTCGAGATGGGCGGTTCCGATATGCACATTCAGTCCGGGGCTCC
GGTCTATTTTCGCATTAGTGGCAAACTGTCCCCGATTAATGAAGAATCTTTAACCCCGCAAGATTGTCAGAAATTGATTT
TTAGTATGCTCAATAATACCCAAAGAAAAGAGTTAGAGCAGAATTGGGAATTAGACTGTTCCTACGGGGTGAAAGGATTA
GCAAGATTTAGGGTCAATGTTTATAAAGAAAGGGGGGCTTATGCAGCTTGTTTACGGGCTTTGTCCTCTAAAATTCCTAA
CTTTGAACAATTAAATTTACCTAGAGTAGTACGGGAGTTATCGGAAAGACCGAGGGGATTAATTCTCGTAACTGGCCAAA
CAGGTTCAGGTAAAACTACCACTTTAGCGGCAATTTTAGACTTAATTAATCGGACTAGGTCTGAACATATTTTAACTGTA
GAAGACCCAATTGAATACGTTTTTCCTAATATAAAAAGTCTGTTTCACCAACGACAAAAAGGAGAAGATACCAAGAGTTT
TGCTAACGCTTTAAGAGCATCTTTACGGGAAGATCCTGATATTATCCTTGTGGGAGAAATGCGGGATTTAGAAACAATTA
GTTTGGCAATCACGGCGGCAGAAACCGGTCACTTAGTCTTCGGAACCCTACACACTAGCTCGGCAGCGGGAACAGTGGAT
CGGATTATTGACGTTTTTCCTGCTAATCAACAGGCACAAATTCGCGCCATGTTATCCAATTCTTTACTAGCAGTTTTTAG
TCAAAACCTAGTCAAGAAAAAAAATCCTAAACCGGGGGAATTTGGTCGGGCAATGGTACAGGAAATCATGGTGGTAACTC
CAGCGATCGCTAACCTAATTCGGGAAGGAAAAGCCCCCCAAGTCTATTCAGCAATTCAAACCGGAATGAAGTTAGGAATG
CAAACCATGGAACAGGGATTAGCTAATTTAGTTGTTAGGGGTGTTGTCTCTTTGGAAGAGGCCATTTCTAAAAGCGGTAA
ACCCGATGAATTACAACGTCTTATCGCCGGGGCCAGCGCTAACGCTAAAGCCCGGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V4BYN4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

52.174

94.262

0.492
  pilT Legionella pneumophila strain ERS1305867

52.174

94.262

0.492
  pilT Pseudomonas aeruginosa PAK

51.297

94.809

0.486
  pilT Acinetobacter baumannii D1279779

51.009

94.809

0.484
  pilT Acinetobacter nosocomialis M2

51.009

94.809

0.484
  pilT Acinetobacter baumannii strain A118

51.009

94.809

0.484
  pilT Acinetobacter baylyi ADP1

50.432

94.809

0.478
  pilT Pseudomonas stutzeri DSM 10701

50.432

94.809

0.478
  pilT Vibrio cholerae strain A1552

50.882

92.896

0.473
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.882

92.896

0.473
  pilT Neisseria meningitidis 8013

49.263

92.623

0.456
  pilT Neisseria gonorrhoeae MS11

48.968

92.623

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.658

97.541

0.445
  pilU Pseudomonas stutzeri DSM 10701

38.462

95.902

0.369

Multiple sequence alignment