Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   LC603172_RS11755 Genome accession   NZ_AP024565
Coordinates   2350529..2352544 (-) Length   671 a.a.
NCBI ID   WP_061432559.1    Uniprot ID   -
Organism   Microcystis aeruginosa strain NIES-88     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2345529..2357544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LC603172_RS11735 (MAN88_22770) - 2347296..2347832 (-) 537 WP_061432556.1 hypothetical protein -
  LC603172_RS11740 (MAN88_22780) - 2347928..2349142 (-) 1215 WP_061432557.1 type II secretion system F family protein -
  LC603172_RS11745 - 2349075..2349284 (+) 210 WP_192846050.1 hypothetical protein -
  LC603172_RS11750 (MAN88_22790) pilT 2349298..2350398 (-) 1101 WP_061432558.1 type IV pilus twitching motility protein PilT Machinery gene
  LC603172_RS11755 (MAN88_22800) pilB/pilB1 2350529..2352544 (-) 2016 WP_061432559.1 GspE/PulE family protein Machinery gene
  LC603172_RS11760 (MAN88_22810) grpE 2352785..2353507 (+) 723 WP_061432560.1 nucleotide exchange factor GrpE -
  LC603172_RS11765 (MAN88_22820) - 2353539..2354258 (-) 720 WP_061432561.1 HAD-IA family hydrolase -
  LC603172_RS11770 (MAN88_22830) - 2354349..2356313 (-) 1965 WP_061432562.1 amylo-alpha-1,6-glucosidase -
  LC603172_RS11775 (MAN88_22840) rpmH 2356404..2356541 (+) 138 WP_002739276.1 50S ribosomal protein L34 -
  LC603172_RS11780 (MAN88_22850) rnpA 2356568..2356930 (+) 363 WP_002763101.1 ribonuclease P protein component -
  LC603172_RS11785 (MAN88_22860) - 2356920..2357315 (+) 396 WP_002738951.1 PH domain-containing protein -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 75060.10 Da        Isoelectric Point: 5.7018

>NTDB_id=86032 LC603172_RS11755 WP_061432559.1 2350529..2352544(-) (pilB/pilB1) [Microcystis aeruginosa strain NIES-88]
MTFSSKKTRAVALRNYFSPFGNKLVQSGYIGADQMQQALVETRKSGRSLVEILQQLTGRPLPPDLQRQYKKNQLFELKIL
YGVESLDPELTDVDGLEIARLIDSLIPIDLCRRYRLIPLRKIEGEPAGILIAMVDPDNLEASDDINRILRLKDLGLQRLV
ITGEDYERLLEKFHSAQSELEQEKARLQKEKELEKLSDLTDIVGNIDVASGGANDEVADDLGEGDANQAPVINLVNKILA
KALQEWASDIHIEPQEEFLKIRFRKDGVLHQAFDPLPKKITPAVTARFKIMADLDIAERRLPQDGKIRRIYQGRKVDFRV
NTLPSRYGEKVCLRILDNSATQLGLDKLITDQKTLQIVRDMASRPFGLLLVTGPTGSGKSTTLYSVLAERNHPGVNISTA
EDPIEYSLPGITQVQVIREKGMDFASILRAFMRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARLD
EMGVEPFMISGSLLGVLAQRLMRRVCTECRVAYHPTQEELARFGLAAANEREVTFYKANTLTLEEIQQARKQGNLCVKCS
GSGYKGRVGVYEVMQNSERLQQLINQGATTDRIKETAVDEGMVTLLAYSLNLVREGYTTLEEVERVTFTDSGLEAELKAK
RKSGLICRGCRAELQPEWLDCPYCMTPRFSD

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=86032 LC603172_RS11755 WP_061432559.1 2350529..2352544(-) (pilB/pilB1) [Microcystis aeruginosa strain NIES-88]
ATGACATTTTCTTCCAAGAAAACCCGTGCCGTTGCCCTGCGCAATTACTTTTCGCCCTTTGGTAACAAGTTGGTGCAGTC
GGGCTATATCGGTGCCGACCAGATGCAACAGGCCCTAGTGGAAACACGGAAATCGGGACGATCGCTAGTAGAAATATTAC
AACAACTAACCGGGCGCCCTTTGCCCCCGGATTTACAGCGACAGTATAAGAAAAACCAGTTATTTGAACTAAAAATTCTC
TACGGAGTCGAATCGCTCGACCCCGAATTAACCGATGTGGATGGACTGGAAATAGCCCGATTGATCGATTCTTTGATTCC
GATCGATCTTTGTCGTCGCTATCGTCTCATCCCCCTGCGAAAGATCGAAGGAGAGCCGGCTGGGATTTTGATAGCCATGG
TCGATCCCGACAACCTCGAGGCTTCCGACGATATTAATCGTATTCTCCGTCTCAAGGATTTAGGTTTACAGCGTTTGGTG
ATTACCGGCGAAGATTATGAAAGATTACTGGAAAAATTCCACTCGGCTCAATCGGAACTAGAACAGGAAAAAGCCCGACT
GCAAAAAGAAAAAGAACTGGAAAAACTCTCGGATCTCACCGATATTGTCGGCAATATTGATGTGGCCTCCGGGGGTGCTA
ACGATGAAGTGGCCGATGATCTCGGAGAAGGGGACGCTAATCAAGCCCCCGTCATCAACCTCGTCAATAAAATTCTAGCT
AAAGCCCTACAGGAATGGGCCTCCGATATTCACATTGAACCCCAAGAAGAATTCCTGAAAATTCGCTTTCGCAAAGATGG
AGTTCTTCATCAAGCTTTTGATCCACTGCCGAAAAAAATTACCCCGGCGGTGACAGCCCGTTTTAAAATCATGGCCGATC
TCGATATTGCCGAACGTCGTCTTCCCCAAGATGGTAAAATTCGGCGGATATATCAAGGGCGGAAAGTGGATTTTCGGGTT
AACACCCTACCCAGTCGCTACGGGGAAAAAGTCTGTTTACGGATTCTCGACAACTCGGCTACCCAGTTGGGTTTAGATAA
ACTAATCACCGACCAAAAGACCCTGCAAATCGTCCGAGACATGGCCAGTCGTCCCTTTGGTCTGTTACTGGTGACAGGCC
CGACCGGTTCGGGGAAATCCACCACTTTATACTCGGTGTTAGCCGAAAGAAATCACCCCGGGGTCAATATTAGTACGGCC
GAAGATCCGATCGAATATTCTCTACCGGGCATCACCCAGGTGCAGGTAATTCGCGAAAAAGGCATGGATTTCGCCTCGAT
TCTGCGGGCATTCATGCGACAAGACCCGGATGTGATTCTGGTGGGAGAAACTAGGGACAAGGAAACGGCCAAAACAGCGA
TCGAGGCGGCCTTAACTGGTCACTTGGTCCTAACTACCCTACACACCAACGATGCCGCAGGTGCGATCGCTCGTCTCGAT
GAGATGGGGGTTGAACCGTTTATGATTTCTGGTTCTCTCTTGGGAGTCTTGGCCCAGCGTCTCATGCGGCGCGTCTGTAC
CGAGTGTCGAGTCGCCTATCACCCCACCCAAGAGGAATTAGCCCGTTTTGGTCTTGCCGCCGCCAATGAACGGGAAGTGA
CCTTTTATAAGGCTAATACCCTAACCCTAGAGGAAATTCAACAGGCGCGAAAACAGGGCAATCTCTGCGTTAAGTGTAGC
GGCAGCGGTTATAAAGGTCGGGTCGGGGTCTATGAGGTAATGCAAAATAGTGAACGACTGCAGCAGTTAATTAACCAAGG
GGCAACCACTGATCGCATTAAGGAGACGGCCGTCGATGAGGGCATGGTGACACTCCTAGCCTATAGTTTAAATCTGGTGC
GCGAGGGTTACACCACCCTCGAAGAGGTGGAACGGGTGACATTTACCGATTCTGGTCTGGAAGCGGAACTAAAAGCCAAA
CGGAAAAGCGGTTTAATCTGTCGTGGTTGTCGCGCCGAACTACAACCAGAATGGCTCGATTGTCCCTATTGTATGACACC
GCGATTTAGTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(237-504)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

74.622

98.659

0.736
  pilF Thermus thermophilus HB27

40.262

91.058

0.367

Multiple sequence alignment