Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q3F88_RS08910 Genome accession   NZ_CP130333
Coordinates   1863681..1864661 (+) Length   326 a.a.
NCBI ID   WP_023295411.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. xiangfangensis strain HD2292     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1858681..1869661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q3F88_RS08875 (Q3F88_08880) - 1859048..1859764 (+) 717 WP_015571842.1 DUF2884 domain-containing protein -
  Q3F88_RS08880 (Q3F88_08885) - 1859924..1860286 (-) 363 WP_023295414.1 endonuclease domain-containing protein -
  Q3F88_RS08885 (Q3F88_08890) hemW 1860343..1861491 (-) 1149 WP_017382926.1 radical SAM family heme chaperone HemW -
  Q3F88_RS08890 (Q3F88_08895) - 1861484..1862077 (-) 594 WP_023295413.1 XTP/dITP diphosphatase -
  Q3F88_RS08895 (Q3F88_08900) yggU 1862081..1862377 (-) 297 WP_003860023.1 DUF167 family protein YggU -
  Q3F88_RS08900 (Q3F88_08905) - 1862374..1862940 (-) 567 WP_015571838.1 YggT family protein -
  Q3F88_RS08905 (Q3F88_08910) - 1862962..1863663 (-) 702 WP_015571837.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q3F88_RS08910 (Q3F88_08915) pilT 1863681..1864661 (+) 981 WP_023295411.1 type IV pilus twitching motility protein PilT Machinery gene
  Q3F88_RS08915 (Q3F88_08920) ruvX 1864671..1865087 (-) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  Q3F88_RS08920 (Q3F88_08925) - 1865087..1865647 (-) 561 WP_006811924.1 YqgE/AlgH family protein -
  Q3F88_RS08925 (Q3F88_08930) gshB 1865722..1866669 (-) 948 WP_023295410.1 glutathione synthase -
  Q3F88_RS08930 (Q3F88_08935) rsmE 1866701..1867432 (-) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Q3F88_RS08935 (Q3F88_08940) endA 1867484..1868191 (-) 708 WP_003860034.1 deoxyribonuclease I -
  Q3F88_RS08940 (Q3F88_08945) - 1868286..1868783 (-) 498 WP_015571833.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35676.61 Da        Isoelectric Point: 6.2335

>NTDB_id=857956 Q3F88_RS08910 WP_023295411.1 1863681..1864661(+) (pilT) [Enterobacter hormaechei subsp. xiangfangensis strain HD2292]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFNHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMFQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=857956 Q3F88_RS08910 WP_023295411.1 1863681..1864661(+) (pilT) [Enterobacter hormaechei subsp. xiangfangensis strain HD2292]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAACCATATGCACGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAGCTGCTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTTCCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCATACGCGCGGCGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCAGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(85-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

47.812

98.16

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374