Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   STYK_RS01045 Genome accession   NZ_AP024523
Coordinates   209420..210361 (+) Length   313 a.a.
NCBI ID   WP_261805080.1    Uniprot ID   -
Organism   Streptococcus toyakuensis strain TP1632     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 204420..215361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STYK_RS01025 (STYK_01810) - 204487..206304 (+) 1818 WP_261805078.1 acyltransferase family protein -
  STYK_RS01030 (STYK_01820) nagA 206456..207607 (+) 1152 WP_001134549.1 N-acetylglucosamine-6-phosphate deacetylase -
  STYK_RS01035 (STYK_01830) - 207770..208828 (+) 1059 WP_261805079.1 zinc-dependent alcohol dehydrogenase family protein -
  STYK_RS01040 (STYK_01840) - 208978..209343 (+) 366 WP_000286405.1 DUF1033 family protein -
  STYK_RS01045 (STYK_01850) comGA/cglA/cilD 209420..210361 (+) 942 WP_261805080.1 competence type IV pilus ATPase ComGA Machinery gene
  STYK_RS01050 (STYK_01860) comGB/cglB 210309..211325 (+) 1017 WP_261805081.1 competence type IV pilus assembly protein ComGB Machinery gene
  STYK_RS01055 (STYK_01870) comGC/cglC 211327..211653 (+) 327 WP_004257466.1 competence type IV pilus major pilin ComGC Machinery gene
  STYK_RS01060 (STYK_01880) comGD/cglD 211646..212050 (+) 405 WP_261081317.1 competence type IV pilus minor pilin ComGD Machinery gene
  STYK_RS01065 (STYK_01890) comGE/cglE 212013..212315 (+) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  STYK_RS01070 (STYK_01900) comGF/cglF 212278..212739 (+) 462 WP_261805082.1 competence type IV pilus minor pilin ComGF Machinery gene
  STYK_RS01075 (STYK_01910) comGG/cglG 212717..213130 (+) 414 WP_261805083.1 competence type IV pilus minor pilin ComGG Machinery gene
  STYK_RS01080 (STYK_01920) - 213163..213750 (+) 588 WP_261805084.1 class I SAM-dependent methyltransferase -
  STYK_RS01085 (STYK_01930) comYH 213811..214764 (+) 954 WP_049515760.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35501.53 Da        Isoelectric Point: 6.2392

>NTDB_id=85709 STYK_RS01045 WP_261805080.1 209420..210361(+) (comGA/cglA/cilD) [Streptococcus toyakuensis strain TP1632]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELYMRVGDERCKIGCYNFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMVSLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIGELGKQYRQRGLYLFSGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVTAEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQPTSWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=85709 STYK_RS01045 WP_261805080.1 209420..210361(+) (comGA/cglA/cilD) [Streptococcus toyakuensis strain TP1632]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGAGCGCAAGACATTTATTTTGTCCCCAAGTT
AGACGCCTATGAGCTTTATATGAGGGTGGGAGACGAGCGCTGTAAAATCGGTTGTTATAATTTTGAAAAGTTTGCGGCCG
TCATCAGTCACTTTAAGTTTGTGGCAGGTATGAATGTTGGGGAAAAGAGACGTAGTCAGCTAGGTTCCTGCGATTATGCC
TATGACCAGAAGATGGTTTCTCTACGTTTATCTACTGTAGGTGATTATCGAGGTCATGAGAGTTTAGTTATTCGCTTGTT
GCATGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGGAGAACTGGGCAAGCAGTATAGGCAACGGGGACTCT
ATCTCTTTTCTGGTCCAGTCGGAAGTGGCAAGACGACCTTGATGCACGAATTGGCTAAATCTCTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTCCAGTTGCAGTTGAATGAAGCGATTGGGCTGAC
CTATGAAAATCTAATCAAACTTTCTTTGCGTCATCGTCCTGATCTCTTGATTATCGGAGAAATTCGGGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGGGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAACGCCTGCTGGAGTTGGGTGTGACTGCGGAGGAATTGGCAGTCGTTCTGCAAGGAGTCTGCTACCAGAGATTAATTGG
GGGAGGAGGAATCGTTGATTTTGCAAACAAAGACTATCAAGAACACCAGCCAACTAGCTGGAATGAACAGATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.486

100

0.965
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

94.569

100

0.946
  comGA/cglA/cilD Streptococcus pneumoniae D39

94.569

100

0.946
  comGA/cglA/cilD Streptococcus pneumoniae R6

94.569

100

0.946
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

94.569

100

0.946
  comYA Streptococcus gordonii str. Challis substr. CH1

78.71

99.042

0.78
  comYA Streptococcus mutans UA159

65.595

99.361

0.652
  comYA Streptococcus mutans UA140

65.595

99.361

0.652
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55

Multiple sequence alignment