Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   STYK_RS01050 Genome accession   NZ_AP024523
Coordinates   210309..211325 (+) Length   338 a.a.
NCBI ID   WP_261805081.1    Uniprot ID   -
Organism   Streptococcus toyakuensis strain TP1632     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 205309..216325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STYK_RS01030 (STYK_01820) nagA 206456..207607 (+) 1152 WP_001134549.1 N-acetylglucosamine-6-phosphate deacetylase -
  STYK_RS01035 (STYK_01830) - 207770..208828 (+) 1059 WP_261805079.1 zinc-dependent alcohol dehydrogenase family protein -
  STYK_RS01040 (STYK_01840) - 208978..209343 (+) 366 WP_000286405.1 DUF1033 family protein -
  STYK_RS01045 (STYK_01850) comGA/cglA/cilD 209420..210361 (+) 942 WP_261805080.1 competence type IV pilus ATPase ComGA Machinery gene
  STYK_RS01050 (STYK_01860) comGB/cglB 210309..211325 (+) 1017 WP_261805081.1 competence type IV pilus assembly protein ComGB Machinery gene
  STYK_RS01055 (STYK_01870) comGC/cglC 211327..211653 (+) 327 WP_004257466.1 competence type IV pilus major pilin ComGC Machinery gene
  STYK_RS01060 (STYK_01880) comGD/cglD 211646..212050 (+) 405 WP_261081317.1 competence type IV pilus minor pilin ComGD Machinery gene
  STYK_RS01065 (STYK_01890) comGE/cglE 212013..212315 (+) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  STYK_RS01070 (STYK_01900) comGF/cglF 212278..212739 (+) 462 WP_261805082.1 competence type IV pilus minor pilin ComGF Machinery gene
  STYK_RS01075 (STYK_01910) comGG/cglG 212717..213130 (+) 414 WP_261805083.1 competence type IV pilus minor pilin ComGG Machinery gene
  STYK_RS01080 (STYK_01920) - 213163..213750 (+) 588 WP_261805084.1 class I SAM-dependent methyltransferase -
  STYK_RS01085 (STYK_01930) comYH 213811..214764 (+) 954 WP_049515760.1 class I SAM-dependent methyltransferase Machinery gene
  STYK_RS01090 (STYK_01940) - 214815..216005 (+) 1191 WP_033684718.1 acetate kinase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38471.40 Da        Isoelectric Point: 9.7344

>NTDB_id=85710 STYK_RS01050 WP_261805081.1 210309..211325(+) (comGB/cglB) [Streptococcus toyakuensis strain TP1632]
MDISQVFRLRRKKLATAKQKNIITLFNSLFSSGFHLVETISFLDRSALLNKQCVTQMRTGLSQGKSFSEMMESLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGFVSVLALSALTFYKRSSKMRVFSILARLPFLGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQALQNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=85710 STYK_RS01050 WP_261805081.1 210309..211325(+) (comGB/cglB) [Streptococcus toyakuensis strain TP1632]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTGTTTAA
CAGTCTCTTTTCCAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCCTTGTTGAACAAGCAGTGTG
TGACCCAGATGCGCACGGGCTTGTCTCAGGGAAAATCATTCTCAGAAATGATGGAAAGTTTGGGCTTTTCAAGTGCTATT
GTCACCCAGTTATCCCTAGCAGAAGTTCATGGGAATCTTCACCTGAGTTTGGGAAAGATAGAAGAGTATCTAGACAATCT
GGCCAAGGTCAAGAAAAAACTAATCGAAGTGGCGACCTATCCTTTGATTTTGCTGGGATTTCTTCTCTTAATTATGCTGG
GGCTACGTAACTACCTACTACCTCAACTGGATAGTAGTAATATTGCCACCCAAATTATCGGCAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGTTTGTTTCCGTACTTGCACTTTCAGCACTCACTTTCTATAAAAGAAGTTCGAAGATGCGCGTCTT
TTCTATCTTAGCACGTCTTCCCTTTCTTGGAATCTTTGTGCAGACCTATTTGACGGCCTATTACGCGCGTGAATGGGGGA
ATATGATTTCGCAGGGAATGGAGCTGACGCAGATTTTTCAGATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGACTTAGCTCAAGCTCTCCAAAATGGTCGTGAGTTTTCTCAAACTATAGGAACCTATCCTTTCTTTAGAAAGGA
ATTGAGTCTCATCATCGAGTATGGGGAAGTCAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCGCTGGTTTTTATCTTTGTGGCTCTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

96.746

100

0.967
  comGB/cglB Streptococcus pneumoniae D39

96.746

100

0.967
  comGB/cglB Streptococcus pneumoniae R6

96.746

100

0.967
  comGB/cglB Streptococcus pneumoniae TIGR4

96.746

100

0.967
  comGB/cglB Streptococcus mitis SK321

95.858

100

0.959
  comGB/cglB Streptococcus mitis NCTC 12261

95.858

100

0.959
  comYB Streptococcus gordonii str. Challis substr. CH1

72.024

99.408

0.716
  comYB Streptococcus mutans UA140

58.36

93.787

0.547
  comYB Streptococcus mutans UA159

58.36

93.787

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50

98.817

0.494

Multiple sequence alignment