Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QUC26_RS26035 Genome accession   NZ_CP128508
Coordinates   5616898..5617896 (-) Length   332 a.a.
NCBI ID   WP_023661483.1    Uniprot ID   V7DB45
Organism   Pseudomonas asiatica strain F19-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5611898..5622896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUC26_RS26015 (QUC26_26015) argS 5613076..5614812 (+) 1737 WP_013974613.1 arginine--tRNA ligase -
  QUC26_RS26020 (QUC26_26020) - 5614814..5615512 (+) 699 WP_003257831.1 SPOR domain-containing protein -
  QUC26_RS26025 (QUC26_26025) - 5615549..5615968 (-) 420 WP_013974614.1 TM2 domain-containing protein -
  QUC26_RS26030 (QUC26_26030) - 5616135..5616770 (+) 636 WP_013974615.1 C40 family peptidase -
  QUC26_RS26035 (QUC26_26035) pilT 5616898..5617896 (-) 999 WP_023661483.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUC26_RS26040 (QUC26_26040) - 5617962..5618648 (+) 687 WP_013974617.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUC26_RS26045 (QUC26_26045) proC 5618687..5619505 (+) 819 WP_023661482.1 pyrroline-5-carboxylate reductase -
  QUC26_RS26050 (QUC26_26050) - 5619516..5620106 (+) 591 WP_013974619.1 YggT family protein -
  QUC26_RS26055 (QUC26_26055) - 5620206..5621345 (+) 1140 WP_013974620.1 homoserine O-acetyltransferase -
  QUC26_RS26060 (QUC26_26060) metW 5621353..5621973 (+) 621 WP_013974621.1 methionine biosynthesis protein MetW -
  QUC26_RS26065 (QUC26_26065) - 5621996..5622430 (+) 435 WP_013974622.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35873.56 Da        Isoelectric Point: 6.3081

>NTDB_id=848159 QUC26_RS26035 WP_023661483.1 5616898..5617896(-) (pilT) [Pseudomonas asiatica strain F19-1]
MDVTDLLARAVDAGASDLHLAAGQLPMLRLDGELQRMALPTLGPVALIEGMAPMLDEDQRRQWAQGDELDLALELPALGR
FRLNLFRQLHGPAATFRLIPGHIATLDELDLGDVFQAVAQCRDGLILVGGPTGSGKSSTLATLLDQLNRDRALHIITLED
PVEVIHSSQRSLVNQREVGRHCGGFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHTRSAVSSVDRL
VEVFAAEEKPLVRAMLAESLRLVVAQMLVRRAGGGRVAAREVLVVTPAVRNLVREGRMAQLCSVMQAGGADGMRTMEGAM
RGLKERGLISDL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=848159 QUC26_RS26035 WP_023661483.1 5616898..5617896(-) (pilT) [Pseudomonas asiatica strain F19-1]
ATGGATGTGACCGACCTGTTGGCCCGGGCCGTGGATGCGGGGGCTTCCGACCTGCACCTGGCGGCGGGCCAGTTACCGAT
GTTGCGCCTGGATGGCGAGCTGCAGCGCATGGCCCTGCCGACCTTGGGCCCGGTCGCCTTGATCGAGGGCATGGCACCCA
TGCTGGATGAAGACCAGCGCCGACAGTGGGCCCAGGGTGATGAGCTGGACCTGGCGCTGGAATTGCCGGCGCTAGGGCGC
TTTCGGCTGAATCTGTTTCGCCAGTTGCACGGCCCTGCGGCCACCTTTCGCCTGATCCCGGGGCATATCGCCACGCTCGA
TGAACTCGACCTGGGCGATGTGTTTCAAGCTGTTGCGCAGTGCCGAGATGGCTTGATCCTCGTCGGTGGGCCGACCGGCA
GTGGCAAGTCCAGCACCTTGGCGACACTGCTCGACCAGCTGAACCGCGATCGTGCTCTGCATATCATCACCCTTGAGGAC
CCTGTCGAAGTTATCCACAGCAGCCAGCGCAGCCTGGTCAACCAGCGTGAGGTCGGCCGCCATTGCGGTGGGTTCGCCCA
GGGGCTGCGCAGTGCCCTGCGCCAAGACCCGGATGTGATCATGATCGGTGAGCTGCGCGACCTGGAAACCATTCGCCTGG
CCTTGCGTGCGGCTGAGACCGGGCACCTGGTACTGGCGACAGTGCATACCCGTTCGGCGGTGAGCAGTGTTGACCGGTTG
GTGGAGGTGTTCGCTGCCGAAGAGAAGCCGTTGGTGCGGGCGATGCTGGCCGAGTCGTTGCGCCTGGTGGTAGCGCAGAT
GCTGGTCAGGCGCGCGGGCGGAGGGCGGGTTGCTGCGCGGGAAGTGCTGGTGGTGACGCCAGCGGTGCGTAACCTGGTTC
GGGAAGGGCGGATGGCGCAGTTGTGTTCGGTGATGCAGGCGGGTGGTGCGGACGGGATGCGGACGATGGAAGGGGCGATG
CGGGGGTTGAAGGAGAGGGGGCTGATCAGCGATCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V7DB45

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.121

99.398

0.518
  pilT Acinetobacter nosocomialis M2

51.212

99.398

0.509
  pilT Acinetobacter baumannii D1279779

51.212

99.398

0.509
  pilT Acinetobacter baumannii strain A118

51.212

99.398

0.509
  pilT Pseudomonas stutzeri DSM 10701

50.909

99.398

0.506
  pilT Acinetobacter baylyi ADP1

50.606

99.398

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.24

99.096

0.488
  pilT Vibrio cholerae strain A1552

49.24

99.096

0.488
  pilT Legionella pneumophila strain Lp02

48.788

99.398

0.485
  pilT Legionella pneumophila strain ERS1305867

48.788

99.398

0.485
  pilT Neisseria meningitidis 8013

48.64

99.699

0.485
  pilT Neisseria gonorrhoeae MS11

48.338

99.699

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.921

99.699

0.458
  pilU Pseudomonas stutzeri DSM 10701

37.16

99.699

0.37
  pilU Vibrio cholerae strain A1552

36.81

98.193

0.361