Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QP609_RS16455 Genome accession   NZ_CP128404
Coordinates   3532165..3532593 (-) Length   142 a.a.
NCBI ID   WP_053495122.1    Uniprot ID   -
Organism   Stenotrophomonas sp. 8(2023)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3527165..3537593
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QP609_RS16440 pilR 3528224..3529597 (+) 1374 WP_014648199.1 sigma-54 dependent transcriptional regulator Regulator
  QP609_RS16445 pilB 3529710..3531440 (-) 1731 WP_014648200.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QP609_RS16450 - 3531665..3532036 (-) 372 WP_229767364.1 pilin -
  QP609_RS16455 pilA 3532165..3532593 (-) 429 WP_053495122.1 pilin Machinery gene
  QP609_RS16460 pilC 3532948..3534207 (+) 1260 WP_053495121.1 type II secretion system F family protein Machinery gene
  QP609_RS16465 - 3534216..3535079 (+) 864 WP_005418626.1 A24 family peptidase -
  QP609_RS16470 coaE 3535091..3535702 (+) 612 WP_053495120.1 dephospho-CoA kinase -
  QP609_RS16475 - 3536678..3536992 (-) 315 WP_258014725.1 hypothetical protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14860.98 Da        Isoelectric Point: 8.4668

>NTDB_id=847257 QP609_RS16455 WP_053495122.1 3532165..3532593(-) (pilA) [Stenotrophomonas sp. 8(2023)]
MKNQKGFTLIELMIVVAIIAILAAIALPQYQNYVAKSQFTAGLAEVSAGKTAYETKVNDGVTDGTQYTTVSNLGLKDSDR
CALTATAPDNTGAGSIVCTLKGNPKVATKKITLERDANGNWSCKSDLAAADKPKYAPATCQG

Nucleotide


Download         Length: 429 bp        

>NTDB_id=847257 QP609_RS16455 WP_053495122.1 3532165..3532593(-) (pilA) [Stenotrophomonas sp. 8(2023)]
ATGAAGAACCAGAAGGGCTTCACCCTTATCGAACTGATGATCGTCGTTGCGATCATTGCAATCCTGGCTGCCATCGCCCT
GCCGCAGTACCAGAACTACGTAGCCAAGTCGCAGTTCACGGCAGGCCTCGCCGAAGTTTCGGCCGGCAAGACTGCCTACG
AAACCAAGGTGAATGACGGTGTCACTGACGGCACGCAGTACACCACTGTATCGAACCTGGGCCTGAAGGATAGCGATCGT
TGCGCGCTCACCGCGACCGCTCCGGATAACACCGGCGCTGGTTCGATTGTGTGCACCTTGAAGGGTAACCCGAAGGTTGC
CACCAAGAAGATCACCTTGGAGCGTGACGCCAACGGCAACTGGAGCTGCAAGTCCGATCTGGCTGCTGCCGACAAGCCGA
AGTATGCTCCGGCTACCTGCCAGGGCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

52.899

97.183

0.514
  pilA/pilAI Pseudomonas stutzeri DSM 10701

48.571

98.592

0.479
  pilA Pseudomonas aeruginosa PAK

46.377

97.183

0.451
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.604

97.887

0.437
  pilA Vibrio cholerae strain A1552

44.604

97.887

0.437
  pilA Vibrio cholerae C6706

44.604

97.887

0.437
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.697

92.958

0.415
  comP Acinetobacter baylyi ADP1

37.908

100

0.408
  pilA Ralstonia pseudosolanacearum GMI1000

34.783

100

0.394
  pilA2 Legionella pneumophila str. Paris

36.667

100

0.387
  pilA2 Legionella pneumophila strain ERS1305867

36

100

0.38
  pilA Vibrio parahaemolyticus RIMD 2210633

42.063

88.732

0.373