Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QP609_RS16445 Genome accession   NZ_CP128404
Coordinates   3529710..3531440 (-) Length   576 a.a.
NCBI ID   WP_014648200.1    Uniprot ID   -
Organism   Stenotrophomonas sp. 8(2023)     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3524710..3536440
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QP609_RS16430 sucC 3524975..3526144 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  QP609_RS16435 - 3526550..3528163 (+) 1614 WP_014648198.1 ATP-binding protein -
  QP609_RS16440 pilR 3528224..3529597 (+) 1374 WP_014648199.1 sigma-54 dependent transcriptional regulator Regulator
  QP609_RS16445 pilB 3529710..3531440 (-) 1731 WP_014648200.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QP609_RS16450 - 3531665..3532036 (-) 372 WP_229767364.1 pilin -
  QP609_RS16455 pilA 3532165..3532593 (-) 429 WP_053495122.1 pilin Machinery gene
  QP609_RS16460 pilC 3532948..3534207 (+) 1260 WP_053495121.1 type II secretion system F family protein Machinery gene
  QP609_RS16465 - 3534216..3535079 (+) 864 WP_005418626.1 A24 family peptidase -
  QP609_RS16470 coaE 3535091..3535702 (+) 612 WP_053495120.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62183.12 Da        Isoelectric Point: 5.8722

>NTDB_id=847256 QP609_RS16445 WP_014648200.1 3529710..3531440(-) (pilB) [Stenotrophomonas sp. 8(2023)]
MNAVTTANLVGITGLARRLVQDGALDEASARDAMAKAAAARQPLPTWFAQNKVVGAAQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGMGNLDVAAGDEDLGAGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLF
LDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPANLPEH
ALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKA
AAGVTSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=847256 QP609_RS16445 WP_014648200.1 3529710..3531440(-) (pilB) [Stenotrophomonas sp. 8(2023)]
ATGAACGCCGTAACCACCGCCAATCTCGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTCCAGGATGGTGCGCTGGACGA
GGCTTCCGCCCGCGACGCCATGGCCAAGGCCGCTGCGGCCCGGCAGCCGCTGCCGACCTGGTTCGCGCAGAACAAGGTGG
TCGGGGCGGCCCAGTTGGCGGCGGCCAACGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACGTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGCGGCAAGCT
GTTCGTGGGTACCAGCAATCCGACCCATTCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTGGAGCCGATCCTGG
TGGATGAAGACCAGATCCGCCGCACGCTCGAGCAGTGGCATGCCAGCCATGACACGCTGGGCGACGCGCTGGGCGGTGAC
GACGATGGCATGGGCAACCTGGATGTTGCGGCCGGAGACGAGGATCTCGGCGCCGGTGGCGACTCCGGCATCGATGCCAA
GGGTGATGACACCCCGGTGGTGAAGTTCGTCAACAAGGTACTGGTGGACGCAATCCGCAAGGGCGCCTCGGACATCCACT
TCGAGCCCTATGAAGACGACTATCGCGTGCGCCTGCGCATCGATGGCCTGCTGAAGATGGTCGCGCGCGCGCCGGTGAAG
CTGAACCAACGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGACG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACGCTGCCGACCCTGTTCGGCGAGAAGGTGG
TGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTGTTC
CTGGACGCCATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTATAC
CGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCGTCA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCACTGCGCTCGTTCCTGCGACAGGATCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTC
CACGCTGCACACCAACGATGCACCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCT
CGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGGTTGTGCAACAACTGCAAGCGCCCGGCCAACCTGCCCGAACAT
GCGTTGCTGGCCGAGGGCTTTACCCAGGCGCAGCTGGATGCGGGTATCGAGCTGTACGAGGCGGTCGGCTGTGACGAGTG
CACCGAGGGGTACAAGGGCCGCACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGCCACGATCGTGCTGGCCG
GTGGCAATGCGTTGCAGATTGCCGAGGCCGCACAGCAGATCGGGGTGAACGATCTGCGCCAGTCGGCGTTGAAGAAGGCG
GCGGCGGGTGTCACCAGCCTGGCCGAGATCAACCGCGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.268

100

0.556
  pilB Acinetobacter baylyi ADP1

54.623

100

0.554
  pilB Legionella pneumophila strain ERS1305867

51.845

98.785

0.512
  pilB Vibrio cholerae strain A1552

48.601

99.306

0.483
  pilF Neisseria gonorrhoeae MS11

48.768

98.611

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

45.455

99.306

0.451
  pilB Vibrio campbellii strain DS40M4

45.518

98.785

0.45