Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QQ994_RS25970 Genome accession   NZ_CP127872
Coordinates   5775836..5776834 (-) Length   332 a.a.
NCBI ID   WP_286100714.1    Uniprot ID   -
Organism   Pseudomonas asiatica strain RYYT.1b     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5770836..5781834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QQ994_RS25950 (QQ994_25950) argS 5771906..5773642 (+) 1737 WP_013974613.1 arginine--tRNA ligase -
  QQ994_RS25955 (QQ994_25955) - 5773644..5774342 (+) 699 WP_025340965.1 SPOR domain-containing protein -
  QQ994_RS25960 (QQ994_25960) - 5774487..5774906 (-) 420 WP_013974614.1 TM2 domain-containing protein -
  QQ994_RS25965 (QQ994_25965) - 5775073..5775708 (+) 636 WP_025340966.1 C40 family peptidase -
  QQ994_RS25970 (QQ994_25970) pilT 5775836..5776834 (-) 999 WP_286100714.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QQ994_RS25975 (QQ994_25975) - 5776900..5777586 (+) 687 WP_286100715.1 YggS family pyridoxal phosphate-dependent enzyme -
  QQ994_RS25980 (QQ994_25980) proC 5777625..5778443 (+) 819 WP_286100716.1 pyrroline-5-carboxylate reductase -
  QQ994_RS25985 (QQ994_25985) - 5778454..5779044 (+) 591 WP_013974619.1 YggT family protein -
  QQ994_RS25990 (QQ994_25990) - 5779144..5780283 (+) 1140 WP_025340970.1 homoserine O-acetyltransferase -
  QQ994_RS25995 (QQ994_25995) metW 5780291..5780911 (+) 621 WP_015272149.1 methionine biosynthesis protein MetW -
  QQ994_RS26000 (QQ994_26000) - 5780934..5781368 (+) 435 WP_286100717.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35808.38 Da        Isoelectric Point: 6.1023

>NTDB_id=844967 QQ994_RS25970 WP_286100714.1 5775836..5776834(-) (pilT) [Pseudomonas asiatica strain RYYT.1b]
MDVTDLLARAVDAGASDLHLAAGQIPMLRLDGELQRMALPTLGPAALTEGMAPMLDEDQRRQWAQGDELDLALELPVLGR
FRLNLFRQLHGPAATFRLIPGHIATLDELDLGDVFQAVAQCSDGLILVGGLTGSGKSSTLATLLDQLNRDRALHIITLED
PVEVIHSSQRSLVNQREVGRHCGGFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHTRSAVSSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVRRAGGGRVAAREVLVVTSAVRNLVREGRMAQLCSVMQAGGADGMRTMEGAM
RGLRERGLISDL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=844967 QQ994_RS25970 WP_286100714.1 5775836..5776834(-) (pilT) [Pseudomonas asiatica strain RYYT.1b]
ATGGATGTGACCGACCTGTTGGCCCGGGCCGTGGATGCGGGGGCTTCCGACCTGCACCTGGCGGCGGGCCAGATACCGAT
GTTGCGCCTGGATGGCGAGCTGCAGCGCATGGCCCTGCCGACCTTGGGCCCTGCCGCCTTGACCGAGGGCATGGCCCCCA
TGCTGGATGAAGACCAGCGCCGACAGTGGGCCCAGGGTGATGAGCTGGACCTGGCGCTGGAATTGCCGGTGCTGGGGCGC
TTTCGGCTGAACCTGTTTCGTCAGTTGCATGGCCCTGCGGCCACCTTTCGCCTGATCCCGGGGCATATCGCCACGCTCGA
TGAACTCGACCTGGGGGATGTGTTTCAAGCTGTTGCGCAGTGCAGCGATGGCTTGATCCTCGTGGGTGGGCTGACCGGCA
GCGGCAAGTCCAGCACCTTGGCGACACTGCTTGACCAGCTGAACCGCGATCGGGCACTGCATATCATCACCCTCGAAGAC
CCTGTCGAAGTTATCCACAGTAGCCAGCGCAGCCTGGTCAACCAGCGTGAGGTCGGCCGCCATTGCGGTGGCTTCGCCCA
GGGGCTGCGCAGTGCCCTGCGCCAGGACCCGGATGTGATCATGATCGGTGAGCTTCGCGACCTGGAAACCATTCGCCTGG
CCTTGCGCGCGGCCGAGACCGGACACCTGGTGTTGGCGACTGTGCATACGCGTTCGGCGGTGAGCAGTATTGACCGGTTG
GTGGAGGTGTTCGCTGCCGAAGAGAAGCCGCTGGTGCGGGCGATGCTGGCCGAGTCGTTGCGCCTGGTGGTAGCGCAGGT
GCTGGTCAGGCGTGCGGGCGGTGGGCGGGTTGCTGCGCGGGAAGTGCTGGTGGTGACGTCAGCGGTGCGTAACCTGGTTC
GGGAGGGGCGGATGGCGCAGTTGTGCTCGGTGATGCAGGCGGGTGGTGCGGACGGGATGCGGACGATGGAAGGGGCGATG
CGGGGGCTGAGGGAGCGAGGGCTGATCAGCGATCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.818

99.398

0.515
  pilT Acinetobacter nosocomialis M2

50.909

99.398

0.506
  pilT Acinetobacter baumannii D1279779

50.909

99.398

0.506
  pilT Acinetobacter baumannii strain A118

50.909

99.398

0.506
  pilT Pseudomonas stutzeri DSM 10701

50.606

99.398

0.503
  pilT Acinetobacter baylyi ADP1

50.303

99.398

0.5
  pilT Legionella pneumophila strain Lp02

48.788

99.398

0.485
  pilT Legionella pneumophila strain ERS1305867

48.788

99.398

0.485
  pilT Vibrio cholerae strain A1552

48.936

99.096

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.936

99.096

0.485
  pilT Neisseria meningitidis 8013

47.734

99.699

0.476
  pilT Neisseria gonorrhoeae MS11

47.432

99.699

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.619

99.699

0.455
  pilU Pseudomonas stutzeri DSM 10701

36.858

99.699

0.367
  pilF Thermus thermophilus HB27

31.282

100

0.367