Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VZG88_RS07015 Genome accession   NZ_CP143871
Coordinates   1430347..1431453 (-) Length   368 a.a.
NCBI ID   WP_323368531.1    Uniprot ID   -
Organism   Synechococcus elongatus IITB6     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1425347..1436453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VZG88_RS06985 (VZG88_06985) - 1425793..1426116 (+) 324 WP_208676183.1 phasin family protein -
  VZG88_RS06990 (VZG88_06990) - 1426171..1426695 (+) 525 WP_208676181.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  VZG88_RS06995 (VZG88_06995) lipB 1426768..1427505 (-) 738 WP_338438675.1 lipoyl(octanoyl) transferase LipB -
  VZG88_RS07000 (VZG88_07000) raiA 1427711..1428334 (+) 624 WP_208676179.1 ribosome-associated translation inhibitor RaiA -
  VZG88_RS07005 (VZG88_07005) larC 1428391..1429599 (-) 1209 WP_208678817.1 nickel pincer cofactor biosynthesis protein LarC -
  VZG88_RS07010 (VZG88_07010) - 1429615..1430316 (+) 702 WP_208678816.1 L-threonylcarbamoyladenylate synthase -
  VZG88_RS07015 (VZG88_07015) pilT 1430347..1431453 (-) 1107 WP_323368531.1 type IV pilus twitching motility protein PilT Machinery gene
  VZG88_RS07020 (VZG88_07020) queA 1431621..1432700 (-) 1080 WP_208678814.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  VZG88_RS07025 (VZG88_07025) - 1432746..1433795 (+) 1050 WP_228383227.1 cupin -
  VZG88_RS07030 (VZG88_07030) - 1433792..1434577 (-) 786 WP_338437627.1 TIGR01548 family HAD-type hydrolase -
  VZG88_RS07035 (VZG88_07035) - 1435010..1435348 (+) 339 WP_208676167.1 30S ribosomal protein PSRP-3 -
  VZG88_RS07040 (VZG88_07040) - 1435476..1436417 (-) 942 WP_208679089.1 HEAT repeat domain-containing protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41030.99 Da        Isoelectric Point: 5.3135

>NTDB_id=844583 VZG88_RS07015 WP_323368531.1 1430347..1431453(-) (pilT) [Synechococcus elongatus IITB6]
MPAPAPAASPQPAASKQPSLAEIVRQAYEHDYSDVHVGVGEVPRFRDRGEIVQTTYPVTDTATFNAWLREVLTEEQVREF
EQGLDFDGATQYDFARVRINIFGSLRGPSMVLRLIPLRILSLDELSLPPVFRDICYYPKGLVLVTGPTGSGKSTTLAAMI
DFINQEMAKNIITIEDPIEFVHQSQRSLIKQREVGVHTLKFEAALRASLREDPDIILVGELRDRETINTALKAAQTGHLV
MATLHTNSAVKTIERVLNMFEPAEQPPVRVALAESLVAIIAQGLCRTIQGKRAAYHEILINTDAIKDYIIRGELEEVEQL
IPKCTFDGMCTSNQSLFRLFNEGRISEETALSQSSKPNEMSQMLRGRV

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=844583 VZG88_RS07015 WP_323368531.1 1430347..1431453(-) (pilT) [Synechococcus elongatus IITB6]
ATGCCTGCGCCTGCCCCAGCCGCATCTCCTCAGCCTGCGGCCAGTAAGCAGCCGAGCTTGGCAGAAATTGTCCGACAAGC
CTACGAGCACGACTACTCGGATGTGCACGTGGGGGTTGGAGAAGTACCGCGCTTCCGCGATCGCGGCGAGATTGTACAAA
CGACCTATCCGGTTACGGATACCGCCACGTTTAATGCTTGGCTGCGCGAAGTACTGACGGAAGAACAGGTGCGCGAATTT
GAGCAGGGCTTAGACTTCGACGGCGCAACCCAATACGACTTTGCGCGGGTTCGGATCAACATCTTTGGGTCGCTGCGGGG
CCCTTCCATGGTGTTGCGGTTAATTCCGCTGCGCATTCTCAGCCTCGATGAGTTAAGCCTCCCGCCGGTCTTCCGCGATA
TTTGCTACTACCCCAAAGGACTGGTGCTCGTCACGGGCCCTACGGGATCGGGGAAATCAACGACCCTCGCCGCCATGATT
GACTTCATCAATCAGGAAATGGCGAAAAATATCATCACGATTGAGGATCCCATCGAGTTTGTTCACCAGAGCCAGCGATC
GCTGATTAAGCAACGCGAAGTCGGTGTCCATACGCTGAAGTTTGAGGCGGCTTTGCGAGCGTCGTTGCGGGAAGACCCTG
ACATTATCCTCGTTGGTGAGTTGCGCGATCGCGAAACCATTAATACAGCCCTGAAAGCTGCTCAAACCGGTCACTTGGTG
ATGGCAACACTCCATACCAACAGTGCCGTCAAAACAATTGAGCGGGTTCTCAATATGTTTGAGCCAGCTGAGCAGCCACC
TGTGCGGGTTGCTCTTGCTGAATCTCTGGTGGCGATTATTGCTCAAGGACTCTGCCGCACTATTCAAGGCAAACGAGCTG
CCTATCACGAGATTTTAATCAATACGGATGCCATTAAGGACTACATCATTCGCGGTGAACTGGAAGAGGTGGAGCAGTTA
ATTCCCAAATGTACTTTTGACGGGATGTGCACTAGTAACCAGTCTCTCTTCCGACTCTTTAATGAAGGCAGAATTAGTGA
GGAGACGGCGCTCTCGCAATCGAGTAAGCCCAATGAAATGTCGCAGATGCTGCGGGGGCGCGTTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(23-286)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

46.018

92.12

0.424
  pilT Acinetobacter nosocomialis M2

45.133

92.12

0.416
  pilT Acinetobacter baumannii D1279779

45.133

92.12

0.416
  pilT Acinetobacter baumannii strain A118

45.133

92.12

0.416
  pilT Neisseria meningitidis 8013

45.619

89.946

0.41
  pilT Legionella pneumophila strain Lp02

45.181

90.217

0.408
  pilT Legionella pneumophila strain ERS1305867

45.181

90.217

0.408
  pilT Pseudomonas stutzeri DSM 10701

44.248

92.12

0.408
  pilT Neisseria gonorrhoeae MS11

45.317

89.946

0.408
  pilT Pseudomonas aeruginosa PAK

43.953

92.12

0.405
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.881

91.033

0.399
  pilT Vibrio cholerae strain A1552

43.881

91.033

0.399
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.21

94.293

0.389
  pilU Pseudomonas stutzeri DSM 10701

39.429

95.109

0.375
  pilU Vibrio cholerae strain A1552

39.466

91.576

0.361