Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   COW1_RS16020 Genome accession   NZ_AP024239
Coordinates   3373826..3374251 (-) Length   141 a.a.
NCBI ID   WP_201344757.1    Uniprot ID   A0A7R7GNV2
Organism   Thiohalobacter sp. COW1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3368826..3379251
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  COW1_RS16005 - 3369754..3370893 (-) 1140 WP_201344751.1 glycosyltransferase family 4 protein -
  COW1_RS16010 (TspCOW1_31740) - 3370915..3371760 (-) 846 WP_201344753.1 glycosyltransferase family 2 protein -
  COW1_RS16015 (TspCOW1_31750) - 3371783..3373756 (-) 1974 WP_201344755.1 hypothetical protein -
  COW1_RS16020 (TspCOW1_31760) pilA2 3373826..3374251 (-) 426 WP_201344757.1 pilin Machinery gene
  COW1_RS16025 (TspCOW1_31770) pilR 3374562..3375911 (-) 1350 WP_201344759.1 sigma-54 dependent transcriptional regulator Regulator
  COW1_RS16030 (TspCOW1_31780) - 3376190..3377812 (-) 1623 WP_201344761.1 ATP-binding protein -
  COW1_RS16035 - 3377809..3378048 (-) 240 WP_201344763.1 PP0621 family protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14765.85 Da        Isoelectric Point: 6.1807

>NTDB_id=84257 COW1_RS16020 WP_201344757.1 3373826..3374251(-) (pilA2) [Thiohalobacter sp. COW1]
MKKTQQGFTLIELMIVVAIIGILAAIAIPAYQDYTIRAKVSEIMGLAAKDKSSVSEYYISMGQMPTVAQSGVSTDAGQST
YVSNIAYAQNSTTEGQLTYTVTNLGAATGTIVFVGTGASTGVSWTCNTGTVDAKYLPANCR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=84257 COW1_RS16020 WP_201344757.1 3373826..3374251(-) (pilA2) [Thiohalobacter sp. COW1]
ATGAAGAAGACACAGCAGGGTTTTACCCTTATCGAACTCATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATCGC
AATCCCGGCCTATCAGGATTACACCATCCGGGCGAAAGTCTCAGAAATCATGGGCCTGGCTGCCAAGGACAAGTCGAGCG
TTTCGGAATATTACATCTCCATGGGACAGATGCCCACTGTTGCGCAGTCGGGTGTCAGCACAGACGCAGGTCAGAGCACG
TATGTGTCGAACATCGCATATGCTCAAAATAGCACGACCGAGGGCCAACTCACATACACTGTTACTAACCTCGGCGCAGC
AACTGGCACCATCGTATTTGTAGGAACCGGAGCGTCAACAGGCGTTAGCTGGACCTGTAATACGGGCACTGTCGACGCAA
AATATCTGCCGGCAAACTGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)

Pilin (bacterial filament) (Pilin)

(37-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7R7GNV2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

53.237

98.582

0.525
  pilA Ralstonia pseudosolanacearum GMI1000

43.529

100

0.525
  pilA2 Legionella pneumophila strain ERS1305867

52.518

98.582

0.518
  comP Acinetobacter baylyi ADP1

46.207

100

0.475
  pilE Neisseria gonorrhoeae strain FA1090

40.252

100

0.454
  pilE Neisseria gonorrhoeae MS11

38.125

100

0.433
  pilA/pilA1 Eikenella corrodens VA1

34.969

100

0.404
  pilA Acinetobacter baumannii strain A118

39.286

99.291

0.39
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.007

100

0.39
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.184

100

0.39
  pilA Haemophilus influenzae 86-028NP

38.406

97.872

0.376
  pilA Pseudomonas aeruginosa PAK

35.57

100

0.376
  pilA Vibrio parahaemolyticus RIMD 2210633

40.945

90.071

0.369
  pilA Haemophilus influenzae Rd KW20

36.957

97.872

0.362

Multiple sequence alignment