NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	QOM10_RS24960	Genome accession	NZ_CP126691
Coordinates	5671537..5671968 (-)	Length	143 a.a.
NCBI ID	WP_034153877.1	Uniprot ID	A0A7Z2REX4
Organism	Pseudomonas sp. P108
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5666537..5676968

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QOM10_RS24945 (QOM10_24935)	-	5667364..5670315 (+)	2952	WP_316917123.1	DUF748 domain-containing protein	-
QOM10_RS24950 (QOM10_24940)	-	5670315..5670611 (+)	297	WP_316917124.1	DUF2845 domain-containing protein	-
QOM10_RS24955 (QOM10_24945)	-	5670882..5671238 (-)	357	WP_047600285.1	BON domain-containing protein	-
QOM10_RS24960 (QOM10_24950)	pilA	5671537..5671968 (-)	432	WP_034153877.1	pilin	Machinery gene
QOM10_RS24965 (QOM10_24955)	pilB	5672195..5673895 (+)	1701	WP_150774961.1	type IV-A pilus assembly ATPase PilB	Machinery gene
QOM10_RS24970 (QOM10_24960)	pilC	5673898..5675115 (+)	1218	WP_127929816.1	type II secretion system F family protein	Machinery gene
QOM10_RS24975 (QOM10_24965)	pilD	5675117..5675989 (+)	873	WP_150751695.1	prepilin peptidase	Machinery gene
QOM10_RS24980 (QOM10_24970)	coaE	5675986..5676609 (+)	624	WP_122607921.1	dephospho-CoA kinase	-
QOM10_RS24985 (QOM10_24975)	yacG	5676606..5676806 (+)	201	WP_007909902.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 143 a.a. Molecular weight: 14323.47 Da Isoelectric Point: 8.4936

                                >NTDB_id=839450 QOM10_RS24960 WP_034153877.1 5671537..5671968(-) (pilA) [Pseudomonas sp. P108]

MKKQQGFTLIELLIVVAIIGILATIALPQYSKYQARSKVTAGLAEISALKVPFEDTINQGTDPTLTLVAGSATAATSNCT

LAASGTASTGAGTITCTLLNAPGPVLSKTITLTRSSGTGAGVWTCGTTVAPDYSPKGCTASGT

Nucleotide

Download Length: 432 bp

                                >NTDB_id=839450 QOM10_RS24960 WP_034153877.1 5671537..5671968(-) (pilA) [Pseudomonas sp. P108]

ATGAAAAAACAACAAGGTTTTACTCTGATCGAGCTGCTGATCGTGGTGGCGATCATCGGGATTCTGGCGACCATCGCCTT

GCCGCAGTATTCCAAGTATCAGGCGCGGTCGAAGGTAACGGCGGGGCTGGCGGAGATCAGTGCGCTGAAGGTGCCGTTTG

AAGACACCATCAATCAAGGCACCGATCCTACGCTGACATTGGTTGCGGGTAGTGCGACGGCGGCGACATCCAACTGCACA

CTCGCCGCATCCGGTACTGCATCCACCGGTGCCGGAACCATCACTTGCACACTGCTCAATGCCCCGGGTCCGGTGCTGAG

CAAGACCATCACCCTGACTCGTTCTTCAGGGACCGGCGCGGGTGTATGGACCTGCGGTACCACCGTTGCTCCGGATTATT

CCCCTAAAGGCTGCACCGCCAGCGGTACATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-139)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A7Z2REX4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Vibrio cholerae C6706	45.395	100	0.483
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	45.395	100	0.483
pilA	Vibrio cholerae strain A1552	45.395	100	0.483
pilA	Acinetobacter baumannii strain A118	47.887	99.301	0.476
pilA/pilAI	Pseudomonas stutzeri DSM 10701	44.928	96.503	0.434
pilA	Pseudomonas aeruginosa PAK	41.06	100	0.434
comP	Acinetobacter baylyi ADP1	39.61	100	0.427
pilA/pilAII	Pseudomonas stutzeri DSM 10701	43.478	96.503	0.42
pilA	Vibrio parahaemolyticus RIMD 2210633	40.278	100	0.406
pilA2	Legionella pneumophila str. Paris	40.714	97.902	0.399
pilA2	Legionella pneumophila strain ERS1305867	40.714	97.902	0.399
pilA	Ralstonia pseudosolanacearum GMI1000	34.783	100	0.392

Detailed information NTG0839450