Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QOM10_RS24965 Genome accession   NZ_CP126691
Coordinates   5672195..5673895 (+) Length   566 a.a.
NCBI ID   WP_150774961.1    Uniprot ID   A0A5E6SRS3
Organism   Pseudomonas sp. P108     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5667195..5678895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QOM10_RS24945 (QOM10_24935) - 5667364..5670315 (+) 2952 WP_316917123.1 DUF748 domain-containing protein -
  QOM10_RS24950 (QOM10_24940) - 5670315..5670611 (+) 297 WP_316917124.1 DUF2845 domain-containing protein -
  QOM10_RS24955 (QOM10_24945) - 5670882..5671238 (-) 357 WP_047600285.1 BON domain-containing protein -
  QOM10_RS24960 (QOM10_24950) pilA 5671537..5671968 (-) 432 WP_034153877.1 pilin Machinery gene
  QOM10_RS24965 (QOM10_24955) pilB 5672195..5673895 (+) 1701 WP_150774961.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QOM10_RS24970 (QOM10_24960) pilC 5673898..5675115 (+) 1218 WP_127929816.1 type II secretion system F family protein Machinery gene
  QOM10_RS24975 (QOM10_24965) pilD 5675117..5675989 (+) 873 WP_150751695.1 prepilin peptidase Machinery gene
  QOM10_RS24980 (QOM10_24970) coaE 5675986..5676609 (+) 624 WP_122607921.1 dephospho-CoA kinase -
  QOM10_RS24985 (QOM10_24975) yacG 5676606..5676806 (+) 201 WP_007909902.1 DNA gyrase inhibitor YacG -
  QOM10_RS24990 (QOM10_24980) - 5676812..5677027 (-) 216 WP_007909901.1 hypothetical protein -
  QOM10_RS24995 (QOM10_24985) - 5677093..5677782 (-) 690 WP_150774964.1 energy-coupling factor ABC transporter permease -
  QOM10_RS25000 (QOM10_24990) - 5677975..5678601 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62523.49 Da        Isoelectric Point: 5.9454

>NTDB_id=839451 QOM10_RS24965 WP_150774961.1 5672195..5673895(+) (pilB) [Pseudomonas sp. P108]
MNDIALSGLAKQLVQAELLTEKSAQQASQQAQRNRLSLVSYLVQNKLVKSSQVAEIASEHFGMALLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLSDAIEKFFDTNASGLEDMADVDLDG
LDVESIDDNKQDAIAGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSRSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPVQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKAIEIPRETLIKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDLQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=839451 QOM10_RS24965 WP_150774961.1 5672195..5673895(+) (pilB) [Pseudomonas sp. P108]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCCGAACTGCTCACGGAAAAAAGCGCCCAGCAAGC
CTCGCAACAGGCCCAGCGTAATCGCTTGTCGCTGGTCAGCTATCTGGTGCAGAACAAGCTGGTCAAGAGCTCACAGGTCG
CCGAAATCGCCTCCGAGCATTTCGGCATGGCCTTGCTTGATCTCAATTGCCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGCGAAAAACTCGTGCGCCAGCATCACGCCCTGCCCCTGTGGCGACGTGGCAACAAGCTGTTCGTGGGCATTTCCGA
CCCGAGCAATCATCAAGCGATCAACGACATTCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AGCTCAGCGATGCCATCGAAAAATTCTTCGACACCAACGCCAGCGGTCTGGAGGACATGGCCGATGTCGATCTCGATGGC
CTCGACGTCGAATCCATTGACGACAACAAACAGGATGCCATCGCCGGTCTCGACGCCGACGATGCGCCGGTGGTGCGCTT
CGTGCACAAGATGCTGCTCGACGCGATCAAGAGCGGCTCTTCCGACTTGCACTTCGAACCCTACGAGAAGAACTACCGGG
TAAGGGTGCGCACCGACGGCATTTTGCGCGAGGTGGCCAAGCCACCGATTCAACTGGCCGGGCGCATCGCCGCGCGCCTG
AAAGTCATGGCCAGCCTCGATATCTCGGAACGGCGCAAACCGCAGGACGGGCGGATCAAGATGCGCCTGTCGAAAAGCAG
GTCGATCGATTTTCGGGTCAACACGCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGCG
CACAGATCGGCATCGACGCGCTCGGCTATGAGCCCGTGCAGAAAGACCTGTATATGGCCGCGCTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGGCCCACCGGTTCGGGTAAAACCGTTTCGCTGTACACCGGCCTGAATATTCTCAACACCGTCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGTATCAACCAGGTCAACGTCAATCCCAAACAAG
GGCTCGACTTTGCCCAGGCACTGCGCTCGTTTCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAAACCGCCGAGATCGCGATCAAAGCCGCGCAGACCGGTCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCCGCGGA
AACCCTGACGCGCCTGCACAACATGGGCATTCCCGGCTTCAACATTGCCACCTCGGTCAGCCTGATCATCGCTCAGCGTC
TGGCGCGCAAACTGTGCAGTCACTGCAAGAAAGCCATCGAGATCCCCCGCGAAACCTTGATCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAACGGCGGCTACAAAGGACGCGTGGGGAT
TTATGAAGTGGTAAAGAACACACCAGAGCTGCAACGTTTGATCATGGCCGAAGGCAACTCGCTGGAAATCGACCTGCAGA
TGCGCCGAGACGGCTTCGACGACCTGCGCACCTCCGGGCTGCACAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5E6SRS3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.156

99.647

0.58
  pilB Acinetobacter baylyi ADP1

58.082

99.47

0.578
  pilB Legionella pneumophila strain ERS1305867

56.46

99.823

0.564
  pilB Vibrio cholerae strain A1552

51.15

99.823

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

49.822

99.293

0.495
  pilB Vibrio campbellii strain DS40M4

48.772

100

0.491
  pilF Neisseria gonorrhoeae MS11

49.378

99.47

0.491
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.736

93.816

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

39.575

91.519

0.362
  pilF Thermus thermophilus HB27

36.738

98.587

0.362