Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QN061_RS12835 Genome accession   NZ_CP126305
Coordinates   2750399..2750821 (+) Length   140 a.a.
NCBI ID   WP_158125403.1    Uniprot ID   -
Organism   Vibrio fluvialis strain SD21SC3889PM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2745399..2755821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QN061_RS12810 (QN061_12810) dsbC 2746220..2746984 (-) 765 WP_024374397.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  QN061_RS12815 (QN061_12815) xerD 2747001..2747909 (-) 909 WP_020331983.1 site-specific tyrosine recombinase XerD -
  QN061_RS12820 (QN061_12820) fldB 2748050..2748571 (+) 522 WP_020331982.1 flavodoxin FldB -
  QN061_RS12825 (QN061_12825) ampD 2748576..2749127 (-) 552 WP_020331981.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QN061_RS12830 (QN061_12830) nadC 2749280..2750167 (+) 888 WP_047459093.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QN061_RS12835 (QN061_12835) pilA 2750399..2750821 (+) 423 WP_158125403.1 pilin Machinery gene
  QN061_RS12840 (QN061_12840) pilB 2750828..2752516 (+) 1689 WP_222733531.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QN061_RS12845 (QN061_12845) pilC 2752552..2753778 (+) 1227 WP_143683928.1 type II secretion system F family protein Machinery gene
  QN061_RS12850 (QN061_12850) pilD 2753830..2754699 (+) 870 WP_143683929.1 A24 family peptidase Machinery gene
  QN061_RS12855 (QN061_12855) coaE 2754702..2755310 (+) 609 WP_143683930.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14585.77 Da        Isoelectric Point: 9.4843

>NTDB_id=837432 QN061_RS12835 WP_158125403.1 2750399..2750821(+) (pilA) [Vibrio fluvialis strain SD21SC3889PM]
MNANQRKQQQGFTLIELMIVVAIIGVLAAIAVPQYQKYVAKSEASAALASITGQRVNVESYVITEGVFPTTTQVPVPSSA
LGTITYANAASAAGDIIFTFKSSGVSPDVVSKKVELKRTSAGAWSCSTTVATDLKPKGCS

Nucleotide


Download         Length: 423 bp        

>NTDB_id=837432 QN061_RS12835 WP_158125403.1 2750399..2750821(+) (pilA) [Vibrio fluvialis strain SD21SC3889PM]
ATGAACGCGAATCAACGCAAACAACAGCAGGGCTTTACCCTGATCGAACTGATGATCGTGGTAGCAATTATTGGGGTGTT
GGCAGCAATCGCTGTTCCTCAGTATCAAAAGTATGTTGCAAAAAGTGAGGCTTCAGCAGCACTAGCATCTATAACAGGAC
AGCGAGTTAATGTTGAGTCTTATGTAATAACAGAAGGCGTATTTCCTACAACAACCCAGGTACCCGTACCTTCCTCTGCA
TTGGGAACAATAACCTATGCTAATGCAGCATCTGCTGCTGGGGATATCATATTTACATTCAAATCTTCAGGTGTAAGCCC
AGATGTTGTCAGTAAAAAAGTGGAACTAAAGAGAACAAGTGCTGGTGCATGGAGCTGTAGTACAACTGTAGCGACAGACT
TGAAGCCTAAAGGCTGTTCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)

Pilin (bacterial filament) (Pilin)

(40-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

49.669

100

0.536
  pilA Vibrio cholerae strain A1552

49.669

100

0.536
  pilA Vibrio cholerae C6706

49.669

100

0.536
  pilA Pseudomonas aeruginosa PAK

43.919

100

0.464
  pilA Acinetobacter baumannii strain A118

43.151

100

0.45
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.357

100

0.443
  pilA Vibrio parahaemolyticus RIMD 2210633

39.041

100

0.407
  comP Acinetobacter baylyi ADP1

34.81

100

0.393
  pilA2 Legionella pneumophila strain ERS1305867

35.333

100

0.379
  pilA2 Legionella pneumophila str. Paris

34.667

100

0.371
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.806

95.714

0.371
  pilA Acinetobacter nosocomialis M2

40.8

89.286

0.364