Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QN061_RS12840 Genome accession   NZ_CP126305
Coordinates   2750828..2752516 (+) Length   562 a.a.
NCBI ID   WP_222733531.1    Uniprot ID   -
Organism   Vibrio fluvialis strain SD21SC3889PM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2745828..2757516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QN061_RS12810 (QN061_12810) dsbC 2746220..2746984 (-) 765 WP_024374397.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  QN061_RS12815 (QN061_12815) xerD 2747001..2747909 (-) 909 WP_020331983.1 site-specific tyrosine recombinase XerD -
  QN061_RS12820 (QN061_12820) fldB 2748050..2748571 (+) 522 WP_020331982.1 flavodoxin FldB -
  QN061_RS12825 (QN061_12825) ampD 2748576..2749127 (-) 552 WP_020331981.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QN061_RS12830 (QN061_12830) nadC 2749280..2750167 (+) 888 WP_047459093.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QN061_RS12835 (QN061_12835) pilA 2750399..2750821 (+) 423 WP_158125403.1 pilin Machinery gene
  QN061_RS12840 (QN061_12840) pilB 2750828..2752516 (+) 1689 WP_222733531.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QN061_RS12845 (QN061_12845) pilC 2752552..2753778 (+) 1227 WP_143683928.1 type II secretion system F family protein Machinery gene
  QN061_RS12850 (QN061_12850) pilD 2753830..2754699 (+) 870 WP_143683929.1 A24 family peptidase Machinery gene
  QN061_RS12855 (QN061_12855) coaE 2754702..2755310 (+) 609 WP_143683930.1 dephospho-CoA kinase -
  QN061_RS12860 (QN061_12860) zapD 2755343..2756083 (+) 741 WP_020332196.1 cell division protein ZapD -
  QN061_RS12865 (QN061_12865) yacG 2756138..2756335 (+) 198 WP_020332195.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62007.76 Da        Isoelectric Point: 6.0857

>NTDB_id=837433 QN061_RS12840 WP_222733531.1 2750828..2752516(+) (pilB) [Vibrio fluvialis strain SD21SC3889PM]
MNTNLLAILRQADLLSPTQEQAVMDHIHASGTNVPEALIDLAIFNHHDLVEHISQIFGLPNTDLTRFDFAPLCQQLGLRE
LITRYQALPLNSNGQILTLAVSDPTHLQVEDEFRFATGLQIELVLADHNALQAAIRRLYGRTIASHGTQGKEISQDELAS
LVQVSDEEKTSIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESFYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLNADTAIDMRVSTLPTLWGEKIVLRLLDSSASSLDIDKLGYNDAQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTDEVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAADSVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVATSPSPTLQQQFHFSSQQ
PIYAANPSGCNECTNGYSGRVGIYEVMAFNSALAEAIMQRASIHQIESIAQQHGMQTLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=837433 QN061_RS12840 WP_222733531.1 2750828..2752516(+) (pilB) [Vibrio fluvialis strain SD21SC3889PM]
ATGAACACCAACCTACTCGCCATCCTGCGTCAGGCCGATTTGCTTAGCCCTACGCAGGAACAGGCCGTGATGGATCATAT
TCACGCTTCCGGAACCAATGTTCCGGAAGCGTTGATCGATTTGGCGATCTTTAATCATCACGATCTGGTCGAACATATCA
GCCAGATCTTTGGTTTGCCGAATACTGATCTGACCCGTTTTGACTTCGCGCCGCTTTGCCAGCAGTTGGGGCTGCGCGAG
CTCATCACCCGTTATCAGGCTCTGCCCCTCAACAGCAACGGTCAGATACTGACTCTTGCGGTATCCGATCCTACTCACCT
ACAAGTTGAAGACGAGTTTCGTTTTGCGACCGGGTTACAAATAGAACTGGTGTTGGCTGATCATAATGCACTGCAGGCCG
CTATTCGCCGCTTGTATGGCCGAACCATCGCGTCACATGGCACACAGGGCAAGGAGATCAGCCAGGACGAACTGGCCAGT
CTGGTGCAGGTTTCCGATGAAGAAAAAACATCAATCGAAGATCTGAGTCAGGATGACTCCCCGGTCAGTCGTTTCATCAA
CCAGATCCTGCTGGATGCGGTGCGTAAAGGCGCTTCTGATATCCACTTTGAACCCTATGAATCCTTTTATCGCATTCGCC
TGCGCTGCGACGGCATTTTGGTGGAAACCCAGCAACCAGCCAGCCATTTAAGCCGCCGACTGGCTGCGCGAATTAAGATC
TTATCGAAACTCGATATCGCTGAGCGACGTTTACCGCAAGACGGCCGCATCAAATTACGCCTCAATGCGGACACGGCCAT
TGATATGCGGGTCTCCACTCTGCCAACCTTGTGGGGGGAGAAAATCGTCCTGCGTCTGCTCGACAGCAGTGCGTCCAGTC
TCGATATCGACAAGTTGGGTTATAACGATGCGCAAAAGCAGCTTTATCTGGCGGCCCTCACCAAGCCGCAAGGTATGATT
CTGATGACTGGCCCGACGGGCAGCGGTAAGACAGTCTCACTCTATACCGGCCTTCGCATTCTCAACACCGACGAAGTGAA
TATTTCTACCGCCGAAGACCCGGTGGAAATTAACCTGCCCGGCATTAACCAGGTTCAGGTGCAGCCCAAAATTGGTTTTG
GCTTTGCGCAGGCTCTGCGCTCCTTTCTGCGTCAGGATCCCGATGTGGTGATGGTGGGCGAAATCCGCGATTTGGAAACG
GCAGAGATTGCCGTCAAAGCCGCCCAAACCGGCCACTTGGTGCTATCCACACTGCACACCAACTCCGCCGCCGATAGCGT
GATTCGGCTGGCGAACATGGGCATTGAACCGTTCAACCTCGCTTCATCGCTGAGCCTCATCATCGCTCAACGACTTGCCC
GACGCCTTTGCCCTCACTGCAAAGTCGCCACATCACCCTCTCCGACACTGCAGCAACAATTTCACTTTAGTTCTCAGCAG
CCTATCTATGCCGCCAACCCATCAGGCTGCAACGAGTGCACCAACGGTTACAGCGGGCGGGTGGGGATTTATGAAGTGAT
GGCATTTAACTCCGCGCTGGCTGAAGCGATTATGCAACGAGCCAGCATCCATCAAATCGAATCCATTGCCCAGCAACATG
GCATGCAAACGCTGTTGCAATCGGGGATCGAAAAACTTCGCGCCGGGATCACCAGCTTCAGTGAGTTGCAGCGTGTTCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

81.851

100

0.819
  pilB Vibrio campbellii strain DS40M4

71.352

100

0.714
  pilB Vibrio parahaemolyticus RIMD 2210633

71.174

100

0.712
  pilB Legionella pneumophila strain ERS1305867

51.838

91.993

0.477
  pilB Acinetobacter baumannii D1279779

50.29

91.993

0.463
  pilB Acinetobacter baylyi ADP1

49.231

92.527

0.456
  pilF Neisseria gonorrhoeae MS11

44.681

100

0.448
  pilF Thermus thermophilus HB27

37.367

100

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

37.634

99.288

0.374