Detailed information NTG0083414

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: Hhaem_RS00605
• Genome accession: NZ_AP024093
• Coordinates: 122947..123387 (+)
• Length: 146 a.a.
• NCBI ID: WP_223893697.1
• Uniprot ID: -
• Organism: Haemophilus haemolyticus strain 2019-19
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 117947..128387

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Hhaem_RS00575 (Hhaem_01170)	tadA	118173..118688 (-)	516	WP_223893690.1	tRNA adenosine(34) deaminase TadA	-
Hhaem_RS00580 (Hhaem_01180)	-	118688..119539 (-)	852	WP_223893692.1	thymidylate synthase	-
Hhaem_RS00585 (Hhaem_01190)	lgt	119550..120356 (-)	807	WP_223893693.1	prolipoprotein diacylglyceryl transferase	-
Hhaem_RS00590 (Hhaem_01200)	-	120365..121162 (-)	798	WP_223893694.1	sulfite exporter TauE/SafE family protein	-
Hhaem_RS00595 (Hhaem_01210)	rppH	121162..121752 (-)	591	WP_118813215.1	RNA pyrophosphohydrolase	-
Hhaem_RS00600 (Hhaem_01220)	ampD	122278..122832 (-)	555	WP_223893695.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
Hhaem_RS00605 (Hhaem_01230)	pilA	122947..123387 (+)	441	WP_223893697.1	prepilin peptidase-dependent pilin	Machinery gene
Hhaem_RS00610 (Hhaem_01240)	pilB	123384..124778 (+)	1395	WP_223893698.1	GspE/PulE family protein	Machinery gene
Hhaem_RS00615 (Hhaem_01250)	pilC	124775..125995 (+)	1221	WP_223893699.1	type II secretion system F family protein	Machinery gene
Hhaem_RS00620 (Hhaem_01260)	pilD	125992..126684 (+)	693	WP_223893700.1	A24 family peptidase	Machinery gene
Hhaem_RS00625 (Hhaem_01270)	rho	126738..128000 (-)	1263	WP_005629550.1	transcription termination factor Rho	-

Sequence

Protein

• Length: 146 a.a.
• Molecular weight: 15022.18 Da
• Isoelectric Point: 8.0922

>NTDB_id=83414 Hhaem_RS00605 WP_223893697.1 122947..123387(+) (pilA) [Haemophilus haemolyticus strain 2019-19]
MKLTTQTTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKSDVELCVYSTGKPSSCSGGSNGIA
ADITTAKGYVKSVTTSNGAITVAGNGTLDGMSYTLTAEGDSANGVTWKTTCGTTNADIFPAGFCSK

Nucleotide

• Length: 441 bp

>NTDB_id=83414 Hhaem_RS00605 WP_223893697.1 122947..123387(+) (pilA) [Haemophilus haemolyticus strain 2019-19]
ATGAAACTAACTACACAAACTACCTTGAAAAAAGGCTTTACCTTAATTGAATTGATGATAGTCATCGCCATCATCGCGAT
TTTAGCTACTATCGCTATTCCTTCTTATCAAAATTATACCAAAAAAGCCGCAGTATCCGAATTACTGCAAGCTTCCGCAC
CTTATAAGTCAGATGTGGAATTGTGCGTTTATAGCACAGGTAAACCTTCCAGTTGCTCGGGAGGAAGCAATGGAATTGCA
GCTGATATTACAACAGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAGCAGGTAATGGTAC
ATTAGATGGAATGAGTTATACGCTCACGGCTGAAGGCGATAGTGCAAACGGCGTTACATGGAAAACCACCTGTGGCACAA
CGAATGCCGATATTTTCCCCGCTGGATTCTGTTCAAAATGA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (10-32)

• Pilin (bacterial filament) (Pilin) (42-142)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Haemophilus influenzae 86-028NP	81.25	98.63	0.801
pilA	Haemophilus influenzae Rd KW20	77.778	98.63	0.767
pilA	Glaesserella parasuis strain SC1401	56	100	0.575
pilA2	Legionella pneumophila strain ERS1305867	41.497	100	0.418
pilA2	Legionella pneumophila str. Paris	41.497	100	0.418
comP	Acinetobacter baylyi ADP1	41.379	99.315	0.411
pilE	Neisseria gonorrhoeae MS11	42.754	94.521	0.404
pilA	Vibrio campbellii strain DS40M4	37.671	100	0.377
pilA	Vibrio cholerae strain A1552	37.241	99.315	0.37
pilA	Vibrio cholerae C6706	37.241	99.315	0.37
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	37.241	99.315	0.37
pilA/pilAI	Pseudomonas stutzeri DSM 10701	37.063	97.945	0.363