Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Hhaem_RS00610 Genome accession   NZ_AP024093
Coordinates   123384..124778 (+) Length   464 a.a.
NCBI ID   WP_223893698.1    Uniprot ID   -
Organism   Haemophilus haemolyticus strain 2019-19     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 118384..129778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Hhaem_RS00580 (Hhaem_01180) - 118688..119539 (-) 852 WP_223893692.1 thymidylate synthase -
  Hhaem_RS00585 (Hhaem_01190) lgt 119550..120356 (-) 807 WP_223893693.1 prolipoprotein diacylglyceryl transferase -
  Hhaem_RS00590 (Hhaem_01200) - 120365..121162 (-) 798 WP_223893694.1 sulfite exporter TauE/SafE family protein -
  Hhaem_RS00595 (Hhaem_01210) rppH 121162..121752 (-) 591 WP_118813215.1 RNA pyrophosphohydrolase -
  Hhaem_RS00600 (Hhaem_01220) ampD 122278..122832 (-) 555 WP_223893695.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Hhaem_RS00605 (Hhaem_01230) pilA 122947..123387 (+) 441 WP_223893697.1 prepilin peptidase-dependent pilin Machinery gene
  Hhaem_RS00610 (Hhaem_01240) pilB 123384..124778 (+) 1395 WP_223893698.1 GspE/PulE family protein Machinery gene
  Hhaem_RS00615 (Hhaem_01250) pilC 124775..125995 (+) 1221 WP_223893699.1 type II secretion system F family protein Machinery gene
  Hhaem_RS00620 (Hhaem_01260) pilD 125992..126684 (+) 693 WP_223893700.1 A24 family peptidase Machinery gene
  Hhaem_RS00625 (Hhaem_01270) rho 126738..128000 (-) 1263 WP_005629550.1 transcription termination factor Rho -
  Hhaem_RS00630 (Hhaem_01280) metJ 128248..128565 (+) 318 WP_005634287.1 met regulon transcriptional regulator MetJ -
  Hhaem_RS00635 (Hhaem_01290) cueR 128579..128965 (-) 387 WP_005629546.1 Cu(I)-responsive transcriptional regulator -
  Hhaem_RS00640 (Hhaem_01300) - 129042..129248 (+) 207 WP_223893701.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52712.14 Da        Isoelectric Point: 6.1425

>NTDB_id=83415 Hhaem_RS00610 WP_223893698.1 123384..124778(+) (pilB) [Haemophilus haemolyticus strain 2019-19]
MIDLTSAKPRVKAQNGEIFTISPDLWARNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKLVEPVLL
ESTQLKELLQQLSPNQLQVEEQVKYYQHQEQSHLEQQDDEPVIRLLNQIFESALQNNASDIHLETLSDHFQVRFRIDGVL
QVQPPLSKNLANRIISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKVVLRAQQNKPVELSFSELG
MTESQRQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEVELDGIIQSQINLQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQHEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFKNLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=83415 Hhaem_RS00610 WP_223893698.1 123384..124778(+) (pilB) [Haemophilus haemolyticus strain 2019-19]
ATGATCGACTTAACTAGTGCAAAACCTCGTGTAAAAGCACAAAATGGCGAGATATTTACGATCTCGCCAGATTTATGGGC
GCGTAATCAGCAACAACAGTCGCTACTCTTACGTTATTTTGCCTTACCACTCAAAGAAGAAAATAATCGTCTTTGGTTAG
GAGTCGATTCCCTTTCTAATCTTTCCGCCTGTGAAACTATTGCGTTTATAACTGGTAAGCTTGTTGAGCCAGTTTTATTA
GAAAGCACTCAACTTAAAGAACTATTACAGCAACTTTCCCCTAATCAACTTCAAGTGGAAGAGCAAGTTAAATATTACCA
ACATCAAGAACAGTCTCATCTTGAACAACAAGATGATGAACCCGTCATTCGATTACTTAATCAAATTTTTGAATCCGCTT
TACAAAACAATGCCTCAGATATTCATTTGGAGACCTTATCGGATCATTTCCAAGTGCGGTTTAGAATTGACGGTGTTTTA
CAAGTTCAGCCTCCACTTAGCAAAAATTTGGCGAATCGAATTATTTCTCGCCTAAAACTGCTCGCTAAATTAGATATCAG
TGAAACCCGCCTTCCACAAGATGGACGATTTCAATTTAAAACTACGTTTTCCGATATTCTTGATTTCCGTCTTTCAACGT
TACCAACTCATTGGGGAGAGAAAGTTGTCCTAAGAGCACAGCAAAATAAACCTGTAGAACTCAGCTTTTCTGAACTGGGC
ATGACCGAAAGTCAACGACAAGCATTTCAACGCGCGCTTAGCCAACCACAAGGATTAATTTTAGTAACTGGCCCAACAGG
AAGTGGGAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTGAATACACCAGATAAACATATTATGACCGCTGAGGATC
CCATTGAAGTTGAGCTGGATGGCATTATTCAAAGCCAAATTAATCTACAGATTGGATTAGATTTTAGCCGCCTATTGCGC
GCCTTTTTACGCCAAGATCCAGACATTATTATGTTAGGTGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTACGTGC
CGCCCAAACTGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATATCCGCCATTTCTCGCTTACAACAACTCG
GTATTCAACAACATGAAATTGAAAACAGTTTACTGCTCGTTATTGCTCAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGTTATCAAACGGATTTTAAAAATTTACGTGAGAGTGGTTTAGAAAAAGTTAGCCAAGGTATAACGGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(23-96)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

93.187

98.06

0.914
  pilB Haemophilus influenzae Rd KW20

91.63

97.845

0.897
  pilB Glaesserella parasuis strain SC1401

58.772

98.276

0.578
  pilB Vibrio campbellii strain DS40M4

40.947

100

0.429
  pilB Vibrio cholerae strain A1552

40.825

100

0.427
  pilB Legionella pneumophila strain ERS1305867

39.798

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.541

100

0.42
  pilB Acinetobacter baumannii D1279779

39.337

100

0.409
  pilB Acinetobacter baylyi ADP1

37.675

100

0.405
  pilF Neisseria gonorrhoeae MS11

37.605

100

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.527

100

0.379
  pilF Thermus thermophilus HB27

35.876

100

0.375

Multiple sequence alignment