Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QFX16_RS24730 Genome accession   NZ_CP125619
Coordinates   5360953..5361384 (-) Length   143 a.a.
NCBI ID   WP_283181704.1    Uniprot ID   -
Organism   Pseudomonas svalbardensis strain PMCC 200367     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5355953..5366384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QFX16_RS24715 - 5356892..5359843 (+) 2952 WP_283181702.1 DUF748 domain-containing protein -
  QFX16_RS24720 - 5359843..5360139 (+) 297 WP_283181703.1 DUF2845 domain-containing protein -
  QFX16_RS24725 - 5360288..5360641 (-) 354 WP_274074573.1 BON domain-containing protein -
  QFX16_RS24730 pilA 5360953..5361384 (-) 432 WP_283181704.1 pilin Machinery gene
  QFX16_RS24735 pilB 5361610..5363310 (+) 1701 WP_283181705.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QFX16_RS24740 pilC 5363313..5364530 (+) 1218 WP_283181706.1 type II secretion system F family protein Machinery gene
  QFX16_RS24745 pilD 5364532..5365404 (+) 873 WP_283181707.1 A24 family peptidase Machinery gene
  QFX16_RS24750 coaE 5365401..5366024 (+) 624 WP_283181708.1 dephospho-CoA kinase -
  QFX16_RS24755 yacG 5366021..5366221 (+) 201 WP_008153323.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14274.36 Da        Isoelectric Point: 8.1130

>NTDB_id=831953 QFX16_RS24730 WP_283181704.1 5360953..5361384(-) (pilA) [Pseudomonas svalbardensis strain PMCC 200367]
MNTQKGFTLIELLIVVAIIGILATFALPQYSKYQARAKVTAGLAEISALKVPFEDTINQGTDPTLLLVAGNAAATTSNCT
LAASGTASTGAGTITCTLVNAPGPVLGKTITLTRSSGNTAGAWTCASNVNADFAPKGCPGSGA

Nucleotide


Download         Length: 432 bp        

>NTDB_id=831953 QFX16_RS24730 WP_283181704.1 5360953..5361384(-) (pilA) [Pseudomonas svalbardensis strain PMCC 200367]
ATGAATACTCAAAAAGGTTTTACCCTGATCGAACTGCTGATCGTGGTGGCGATCATCGGGATTCTGGCGACGTTTGCCTT
GCCACAATATTCAAAGTACCAGGCCCGGGCCAAAGTCACGGCGGGGCTGGCTGAGATCAGCGCGTTGAAAGTGCCTTTCG
AGGACACCATCAACCAAGGCACCGACCCTACATTGCTCTTGGTTGCGGGTAACGCGGCGGCGACGACCTCCAATTGCACG
TTGGCGGCTTCAGGCACAGCTTCGACAGGTGCGGGCACGATCACTTGCACGCTGGTTAATGCCCCAGGACCGGTGTTGGG
CAAAACCATCACACTGACTCGCTCGTCAGGAAACACGGCGGGCGCCTGGACGTGTGCCAGCAATGTTAACGCTGACTTCG
CACCAAAAGGCTGTCCCGGCAGCGGCGCGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-138)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

52.143

97.902

0.51
  pilA Pseudomonas aeruginosa PAK

42

100

0.441
  pilA Vibrio cholerae C6706

42.759

100

0.434
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.759

100

0.434
  pilA Vibrio cholerae strain A1552

42.759

100

0.434
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.478

96.503

0.42
  comP Acinetobacter baylyi ADP1

38.562

100

0.413
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.382

95.105

0.413
  pilA2 Legionella pneumophila str. Paris

40.288

97.203

0.392
  pilA2 Legionella pneumophila strain ERS1305867

40.288

97.203

0.392
  pilA Ralstonia pseudosolanacearum GMI1000

36.242

100

0.378
  pilA/pilA1 Eikenella corrodens VA1

39.394

92.308

0.364