Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QFX16_RS24735 Genome accession   NZ_CP125619
Coordinates   5361610..5363310 (+) Length   566 a.a.
NCBI ID   WP_283181705.1    Uniprot ID   -
Organism   Pseudomonas svalbardensis strain PMCC 200367     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5356610..5368310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QFX16_RS24715 - 5356892..5359843 (+) 2952 WP_283181702.1 DUF748 domain-containing protein -
  QFX16_RS24720 - 5359843..5360139 (+) 297 WP_283181703.1 DUF2845 domain-containing protein -
  QFX16_RS24725 - 5360288..5360641 (-) 354 WP_274074573.1 BON domain-containing protein -
  QFX16_RS24730 pilA 5360953..5361384 (-) 432 WP_283181704.1 pilin Machinery gene
  QFX16_RS24735 pilB 5361610..5363310 (+) 1701 WP_283181705.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QFX16_RS24740 pilC 5363313..5364530 (+) 1218 WP_283181706.1 type II secretion system F family protein Machinery gene
  QFX16_RS24745 pilD 5364532..5365404 (+) 873 WP_283181707.1 A24 family peptidase Machinery gene
  QFX16_RS24750 coaE 5365401..5366024 (+) 624 WP_283181708.1 dephospho-CoA kinase -
  QFX16_RS24755 yacG 5366021..5366221 (+) 201 WP_008153323.1 DNA gyrase inhibitor YacG -
  QFX16_RS24760 - 5366225..5366440 (-) 216 WP_033054332.1 hypothetical protein -
  QFX16_RS24765 - 5366504..5367193 (-) 690 WP_283181709.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62806.95 Da        Isoelectric Point: 5.8558

>NTDB_id=831954 QFX16_RS24735 WP_283181705.1 5361610..5363310(+) (pilB) [Pseudomonas svalbardensis strain PMCC 200367]
MNDIALSGLAKQLVLAELLTDKSAQQAYQQAQRNKISLVTYLVQNKLVKSRQVAEIASEHFGMTLLDLSCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGVSDPTNQQAINDIQFSTGLNTEAILVEDDKLSDAIEKFFDTQGTGLEDMADVDLDG
VDIESIDDNKQDTIAGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGILREVAKPPIHLANRIAARL
KVMASLDISERRRPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKELYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSISLIIAQRLARKLCSHCKKPIEIPREALLKEGFPE
ERIGSFTIYEPVGCDHCNNGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=831954 QFX16_RS24735 WP_283181705.1 5361610..5363310(+) (pilB) [Pseudomonas svalbardensis strain PMCC 200367]
ATGAATGACATCGCCCTTAGCGGTCTGGCCAAGCAATTGGTCCTGGCCGAGTTGCTCACTGACAAAAGCGCGCAACAGGC
GTATCAGCAAGCCCAGCGCAACAAAATCTCCCTGGTCACTTACCTGGTGCAGAACAAACTGGTGAAGAGTCGGCAGGTTG
CCGAGATTGCCTCGGAACATTTCGGCATGACGCTGCTTGATCTCAGTTGCCTGGATAAGGAAACACAACCTAAAGGGCTG
GTCAGCGAAAAACTGGTCCGTCAGCACCACGCCCTGCCCCTCTGGCGGCGCGGCAACAAGTTGTTCGTGGGCGTTTCCGA
CCCGACCAATCAGCAAGCCATCAATGACATCCAGTTCAGCACCGGGCTGAATACCGAAGCCATTCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAATTCTTCGATACCCAAGGCACCGGCCTGGAAGACATGGCCGACGTTGACCTCGATGGC
GTGGACATCGAGTCGATCGATGACAACAAACAAGACACCATCGCCGGCCAGGATGCCGATGACGCGCCCGTGGTGCGCTT
CGTTCACAAGATGTTGCTGGACGCGATCAAAAGCGGCTCTTCCGACCTGCATTTCGAGCCCTACGAAAAAACCTACCGCG
TGCGGATGCGCACCGACGGTATTTTGCGTGAAGTCGCCAAGCCACCGATTCACTTGGCCAATCGCATCGCCGCGCGGCTG
AAAGTCATGGCCAGCCTCGACATTTCGGAACGGCGCCGGCCCCAGGACGGGCGGATCAAGATGCGCCTGTCCAAGAGCAA
ATCCATCGACTTTCGGGTCAATACGCTGCCAACTCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGATCCGTCCAGCG
CTCAAATGGGCATCGACGCCCTCGGCTACGAGCCCGACCAGAAAGAACTGTACATGGCCGCCCTCAAGCAGCCGCAGGGG
ATGATTCTGGTGACCGGGCCCACCGGCTCGGGCAAGACCGTATCGCTCTACACCGGGCTGAACATTCTCAACACCGTGGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAAGTCAACGTCAATCCCAAACAGG
GAATGGACTTCGCCCAGGCGTTGCGCTCATTTCTGCGACAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTC
GAAACCGCCGAAATAGCCATTAAAGCCGCCCAGACCGGACACCTGGTGCTGTCGACCCTGCACACCAACAGCGCCGCCGA
AACCCTGACTCGCCTGCACAACATGGGCATTCCGGGTTTCAACATTGCCACCTCGATCAGCCTGATCATTGCCCAGCGCC
TGGCACGCAAGCTGTGCAGCCATTGCAAGAAACCCATCGAGATCCCCCGGGAGGCGTTGCTGAAGGAAGGCTTCCCCGAG
GAACGCATCGGCTCATTCACGATCTATGAGCCAGTCGGTTGCGATCACTGCAACAACGGTTACAAAGGGCGAGTAGGGAT
TTATGAAGTGGTGAAGAACACCCCGGACCTGCAACGGCTGATCATGGCCGAAGGTAACTCACTGGAAATCGACATCCAGA
TGCGTAAGGACGGCTTCAACGACCTGCGGACGTCGGGCCTGCTCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.437

99.47

0.581
  pilB Acinetobacter baumannii D1279779

57.801

99.647

0.576
  pilB Legionella pneumophila strain ERS1305867

55.908

100

0.56
  pilB Vibrio cholerae strain A1552

51.327

99.823

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

50.899

98.233

0.5
  pilB Vibrio campbellii strain DS40M4

49.298

100

0.496
  pilF Neisseria gonorrhoeae MS11

49.378

99.47

0.491
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.752

93.463

0.362