Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QFW87_RS25110 Genome accession   NZ_CP125618
Coordinates   5490922..5491353 (-) Length   143 a.a.
NCBI ID   WP_283181704.1    Uniprot ID   -
Organism   Pseudomonas sp. PMCC200344     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5485922..5496353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QFW87_RS25095 - 5486861..5489812 (+) 2952 WP_283181702.1 DUF748 domain-containing protein -
  QFW87_RS25100 - 5489812..5490108 (+) 297 WP_283189139.1 DUF2845 domain-containing protein -
  QFW87_RS25105 - 5490257..5490610 (-) 354 WP_283189140.1 BON domain-containing protein -
  QFW87_RS25110 pilA 5490922..5491353 (-) 432 WP_283181704.1 pilin Machinery gene
  QFW87_RS25115 pilB 5491579..5493279 (+) 1701 WP_283189141.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QFW87_RS25120 pilC 5493282..5494499 (+) 1218 WP_283189142.1 type II secretion system F family protein Machinery gene
  QFW87_RS25125 pilD 5494501..5495373 (+) 873 WP_283189143.1 A24 family peptidase Machinery gene
  QFW87_RS25130 coaE 5495370..5495993 (+) 624 WP_283189144.1 dephospho-CoA kinase -
  QFW87_RS25135 yacG 5495990..5496190 (+) 201 WP_008153323.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14274.36 Da        Isoelectric Point: 8.1130

>NTDB_id=831923 QFW87_RS25110 WP_283181704.1 5490922..5491353(-) (pilA) [Pseudomonas sp. PMCC200344]
MNTQKGFTLIELLIVVAIIGILATFALPQYSKYQARAKVTAGLAEISALKVPFEDTINQGTDPTLLLVAGNAAATTSNCT
LAASGTASTGAGTITCTLVNAPGPVLGKTITLTRSSGNTAGAWTCASNVNADFAPKGCPGSGA

Nucleotide


Download         Length: 432 bp        

>NTDB_id=831923 QFW87_RS25110 WP_283181704.1 5490922..5491353(-) (pilA) [Pseudomonas sp. PMCC200344]
ATGAATACTCAAAAAGGTTTTACCCTGATCGAACTGCTGATCGTGGTGGCGATCATCGGGATTCTGGCGACGTTTGCCTT
GCCACAATATTCAAAGTACCAGGCCCGGGCCAAAGTCACGGCGGGGCTGGCTGAGATCAGCGCGTTGAAAGTGCCTTTCG
AGGACACCATCAACCAAGGCACCGACCCTACATTGCTCTTGGTTGCGGGTAACGCGGCGGCGACGACCTCCAATTGCACG
TTGGCGGCTTCAGGCACAGCTTCGACAGGTGCGGGCACGATCACTTGCACGCTGGTTAATGCCCCAGGACCGGTGTTGGG
CAAAACCATCACACTGACTCGCTCGTCAGGAAACACGGCGGGCGCCTGGACGTGTGCCAGCAATGTTAACGCTGACTTCG
CACCAAAAGGCTGTCCCGGCAGCGGCGCGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-138)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

52.143

97.902

0.51
  pilA Pseudomonas aeruginosa PAK

42

100

0.441
  pilA Vibrio cholerae C6706

42.759

100

0.434
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.759

100

0.434
  pilA Vibrio cholerae strain A1552

42.759

100

0.434
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.478

96.503

0.42
  comP Acinetobacter baylyi ADP1

38.562

100

0.413
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.382

95.105

0.413
  pilA2 Legionella pneumophila str. Paris

40.288

97.203

0.392
  pilA2 Legionella pneumophila strain ERS1305867

40.288

97.203

0.392
  pilA Ralstonia pseudosolanacearum GMI1000

36.242

100

0.378
  pilA/pilA1 Eikenella corrodens VA1

39.394

92.308

0.364