Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QFW87_RS25115 Genome accession   NZ_CP125618
Coordinates   5491579..5493279 (+) Length   566 a.a.
NCBI ID   WP_283189141.1    Uniprot ID   -
Organism   Pseudomonas sp. PMCC200344     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5486579..5498279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QFW87_RS25095 - 5486861..5489812 (+) 2952 WP_283181702.1 DUF748 domain-containing protein -
  QFW87_RS25100 - 5489812..5490108 (+) 297 WP_283189139.1 DUF2845 domain-containing protein -
  QFW87_RS25105 - 5490257..5490610 (-) 354 WP_283189140.1 BON domain-containing protein -
  QFW87_RS25110 pilA 5490922..5491353 (-) 432 WP_283181704.1 pilin Machinery gene
  QFW87_RS25115 pilB 5491579..5493279 (+) 1701 WP_283189141.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QFW87_RS25120 pilC 5493282..5494499 (+) 1218 WP_283189142.1 type II secretion system F family protein Machinery gene
  QFW87_RS25125 pilD 5494501..5495373 (+) 873 WP_283189143.1 A24 family peptidase Machinery gene
  QFW87_RS25130 coaE 5495370..5495993 (+) 624 WP_283189144.1 dephospho-CoA kinase -
  QFW87_RS25135 yacG 5495990..5496190 (+) 201 WP_008153323.1 DNA gyrase inhibitor YacG -
  QFW87_RS25140 - 5496194..5496409 (-) 216 WP_033054332.1 hypothetical protein -
  QFW87_RS25145 - 5496473..5497162 (-) 690 WP_283181709.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62773.83 Da        Isoelectric Point: 5.7208

>NTDB_id=831924 QFW87_RS25115 WP_283189141.1 5491579..5493279(+) (pilB) [Pseudomonas sp. PMCC200344]
MNDIALSGLAKQLVLAELLTDKSAQQAYQQAQRNKISLVTYLVQNKLVKSRQVAEIASEHFGMTLLDLSCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGVSDPTNQQAINDIQFSTGLNTEAILVEDDKLSDAIEKFFDTQGTGLEDMADVDLDG
VDIESIDDNKQDTIAGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGILREVAKPPIHLANRIAARL
KVMASLDISERRRPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKELYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSISLIIAQRLARKLCSHCKKPIEIPREALLREGFPE
ERIGSFTIYEPVGCDQCNGGYKGRVGIYEVVKNTQDLQRLIMAEGNSLEIDSQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=831924 QFW87_RS25115 WP_283189141.1 5491579..5493279(+) (pilB) [Pseudomonas sp. PMCC200344]
ATGAATGACATCGCCCTTAGCGGTCTGGCCAAGCAATTGGTCCTGGCCGAGTTGCTCACTGACAAAAGCGCGCAACAGGC
GTATCAGCAAGCCCAGCGCAACAAAATCTCCCTGGTCACTTACCTGGTGCAGAACAAACTGGTGAAGAGTCGGCAGGTTG
CCGAGATTGCCTCGGAACATTTCGGCATGACGCTGCTTGATCTCAGTTGCCTGGATAAGGAAACACAACCTAAAGGGCTG
GTCAGCGAAAAACTGGTCCGTCAGCACCACGCCCTGCCCCTCTGGCGGCGCGGCAACAAGTTGTTCGTGGGCGTTTCCGA
CCCGACCAATCAGCAAGCCATCAATGACATCCAGTTCAGCACCGGGCTGAATACCGAAGCCATTCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAATTCTTCGATACCCAAGGCACCGGCCTGGAAGACATGGCCGACGTTGACCTCGATGGC
GTGGACATCGAGTCGATCGATGACAACAAACAAGACACCATCGCCGGCCAGGATGCCGATGACGCGCCCGTGGTGCGCTT
CGTTCACAAGATGTTGCTGGACGCGATCAAAAGCGGCTCTTCCGACCTGCATTTCGAGCCCTACGAAAAAACCTACCGCG
TGCGGATGCGCACCGACGGTATTTTGCGTGAAGTCGCCAAGCCACCGATTCACTTGGCCAATCGCATCGCCGCGCGGCTG
AAAGTCATGGCCAGCCTCGACATCTCGGAACGGCGCCGGCCCCAGGACGGGCGGATCAAGATGCGCCTGTCCAAGAGCAA
ATCCATCGACTTTCGGGTCAATACGCTGCCAACTCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGATCCGTCCAGCG
CTCAAATGGGCATCGACGCCCTCGGCTACGAGCCCGACCAGAAAGAACTGTACATGGCCGCCCTCAAGCAGCCGCAGGGG
ATGATTCTGGTGACCGGGCCGACCGGCTCGGGCAAGACCGTATCGCTCTACACCGGGCTGAACATTCTCAACACCGTGGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAGCAGG
GAATGGACTTCGCCCAAGCGCTGCGCTCATTTCTGCGACAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTT
GAAACCGCCGAAATCGCCATCAAAGCCGCCCAGACCGGACACCTGGTGCTGTCGACCCTGCACACCAACAGCGCCGCCGA
AACCCTGACTCGCCTGCACAACATGGGCATTCCGGGTTTCAACATTGCCACCTCGATCAGCCTGATCATTGCCCAGCGCC
TGGCACGCAAGCTGTGCAGCCATTGCAAGAAACCCATAGAGATTCCCCGGGAGGCGCTGCTCAGGGAAGGCTTCCCCGAG
GAACGCATCGGCTCATTCACGATCTATGAACCGGTCGGTTGCGATCAGTGCAACGGCGGTTACAAAGGACGAGTCGGGAT
TTATGAAGTGGTGAAGAACACACAGGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCACTGGAAATCGACAGTCAGA
TGCGTAAAGACGGCTTCAACGACCTGCGGACGTCGGGCTTGCTCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.259

99.47

0.58
  pilB Acinetobacter baumannii D1279779

57.624

99.647

0.574
  pilB Legionella pneumophila strain ERS1305867

56.085

100

0.562
  pilB Vibrio cholerae strain A1552

51.504

99.823

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

50.899

98.233

0.5
  pilB Vibrio campbellii strain DS40M4

49.298

100

0.496
  pilF Neisseria gonorrhoeae MS11

49.378

99.47

0.491
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.13

93.463

0.366
  pilF Thermus thermophilus HB27

36.283

99.823

0.362