Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QCL67_RS00235 Genome accession   NZ_CP122442
Coordinates   44159..45184 (+) Length   341 a.a.
NCBI ID   WP_182268812.1    Uniprot ID   A0A839BXE3
Organism   Enterobacter ludwigii strain I9455333cz     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 39159..50184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QCL67_RS00195 (QCL67_00195) - 39240..39566 (+) 327 WP_020883688.1 YggL family protein -
  QCL67_RS00200 (QCL67_00200) - 39623..40339 (+) 717 WP_020883689.1 DUF2884 domain-containing protein -
  QCL67_RS00205 (QCL67_00205) - 40401..40763 (-) 363 WP_182268811.1 DUF559 domain-containing protein -
  QCL67_RS00210 (QCL67_00210) hemW 40827..41969 (-) 1143 WP_014171573.1 radical SAM family heme chaperone HemW -
  QCL67_RS00215 (QCL67_00215) - 41962..42555 (-) 594 WP_020883692.1 XTP/dITP diphosphatase -
  QCL67_RS00220 (QCL67_00220) yggU 42559..42855 (-) 297 WP_014885193.1 DUF167 family protein YggU -
  QCL67_RS00225 (QCL67_00225) - 42852..43418 (-) 567 WP_014171570.1 YggT family protein -
  QCL67_RS00230 (QCL67_00230) - 43440..44141 (-) 702 WP_020883693.1 YggS family pyridoxal phosphate-dependent enzyme -
  QCL67_RS00235 (QCL67_00235) pilT 44159..45184 (+) 1026 WP_182268812.1 type IV pilus twitching motility protein PilT Machinery gene
  QCL67_RS00240 (QCL67_00240) ruvX 45150..45566 (-) 417 WP_014171567.1 Holliday junction resolvase RuvX -
  QCL67_RS00245 (QCL67_00245) - 45566..46126 (-) 561 WP_182268813.1 YqgE/AlgH family protein -
  QCL67_RS00250 (QCL67_00250) gshB 46213..47160 (-) 948 WP_020883696.1 glutathione synthase -
  QCL67_RS00255 (QCL67_00255) rsmE 47200..47931 (-) 732 WP_020883697.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QCL67_RS00260 (QCL67_00260) endA 47984..48691 (-) 708 WP_073396422.1 deoxyribonuclease I -
  QCL67_RS00265 (QCL67_00265) - 48786..49283 (-) 498 WP_182268814.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37147.20 Da        Isoelectric Point: 6.0946

>NTDB_id=815495 QCL67_RS00235 WP_182268812.1 44159..45184(+) (pilT) [Enterobacter ludwigii strain I9455333cz]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRAGRLEPAPFPPPEVDTLLKAWLNDEQQGTWWANGQVDFAVTLAGNQRLRG
SVFKQTSGVSMTLRLLPGVCPQLSALGVPRVVPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FLYQSERCLIQQREIGQHSPSFADALRGALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDAQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQSGMQNFDQSLAER
RAQGQLQAPALNNPVRNSFRE

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=815495 QCL67_RS00235 WP_182268812.1 44159..45184(+) (pilT) [Enterobacter ludwigii strain I9455333cz]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGAGCAGGTCGTCTTGAACCCGCGCCGTTTCCGCCTCCCGAAGTCGATACGCTATTAAAGGCGTGGCTCAACG
ATGAACAGCAGGGAACGTGGTGGGCGAACGGGCAGGTGGATTTTGCCGTTACCCTGGCGGGCAATCAGCGCCTGCGCGGA
AGCGTGTTTAAACAGACAAGCGGCGTCTCAATGACGCTGAGGCTGTTACCCGGGGTCTGCCCGCAACTCTCCGCGTTGGG
GGTGCCACGGGTTGTTCCTGAGCTGTTATCCAGTGACAACGGGCTAATTCTGGTCACCGGGGCAACCGGCAGCGGTAAGT
CCACTACGCTGGCAGCGATGGTGGATTTTCTTAATCACCATACTGACGGGCATATTCTGACGCTCGAAGACCCGGTGGAG
TTTCTGTACCAGAGCGAACGGTGCCTGATCCAGCAGCGCGAGATTGGCCAACACAGCCCGTCATTTGCCGACGCGCTGCG
CGGGGCGTTACGCCAGGATCCTGATGTCATACTGCTGGGTGAGCTGCGCGACAGTGAAACAATCCGCCTCGCGCTGACGG
CGGCGGAAACCGGACATCTGGTGCTCGCGACACTGCATACCCGCGGGGCAGCGCAGGCGATTGAGCGGCTGGTCGATACG
TTTCCGGCGCAGGAGAAAGATCCGGTGCGCAATCAGCTGGCTGGCAGCCTGCGGGCAGTGCTGGCGCAAAAGCTCCTCCC
CGATGCACAGGGCGGGCGCGTGGCGCTTTATGAGCTGCTGGTGAATACTCCGGCGGCGGCGAATCTCATTCGCGAAGGAA
AAACGTGGCAATTGCCCGGTATTATTCAAACCGGCCAGCAGTCGGGGATGCAGAACTTTGACCAGAGCCTGGCTGAAAGA
CGGGCGCAGGGGCAACTCCAGGCCCCGGCGCTCAATAACCCTGTTCGAAATAGCTTTCGAGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A839BXE3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.817

99.12

0.484
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.817

99.12

0.484
  pilT Neisseria meningitidis 8013

48.348

97.654

0.472
  pilT Neisseria gonorrhoeae MS11

46.784

100

0.469
  pilT Legionella pneumophila strain Lp02

48.758

94.428

0.46
  pilT Legionella pneumophila strain ERS1305867

48.758

94.428

0.46
  pilT Acinetobacter baylyi ADP1

47.289

97.361

0.46
  pilT Pseudomonas stutzeri DSM 10701

47.401

95.894

0.455
  pilT Acinetobacter baumannii D1279779

47.095

95.894

0.452
  pilT Acinetobacter baumannii strain A118

47.095

95.894

0.452
  pilT Pseudomonas aeruginosa PAK

47.095

95.894

0.452
  pilT Acinetobacter nosocomialis M2

47.5

93.842

0.446
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.099

94.428

0.416
  pilU Pseudomonas stutzeri DSM 10701

40

96.774

0.387
  pilU Vibrio cholerae strain A1552

39.13

94.428

0.369