Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PWH52_RS18320 Genome accession   NZ_CP119084
Coordinates   3926783..3927796 (-) Length   337 a.a.
NCBI ID   WP_338463945.1    Uniprot ID   -
Organism   Franconibacter daqui strain HNU6-13     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3921783..3932796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWH52_RS18300 - 3922633..3923196 (+) 564 WP_024551335.1 YqgE/AlgH family protein -
  PWH52_RS18305 ruvX 3923196..3923612 (+) 417 WP_024555563.1 Holliday junction resolvase RuvX -
  PWH52_RS18310 - 3923776..3924468 (-) 693 WP_338463943.1 IclR family transcriptional regulator -
  PWH52_RS18315 - 3924524..3926782 (+) 2259 WP_338463944.1 FAD-dependent oxidoreductase -
  PWH52_RS18320 pilT 3926783..3927796 (-) 1014 WP_338463945.1 type IV pilus twitching motility protein PilT Machinery gene
  PWH52_RS18325 - 3927815..3928528 (+) 714 WP_024555567.1 YggS family pyridoxal phosphate-dependent enzyme -
  PWH52_RS18330 - 3928544..3929098 (+) 555 WP_024555568.1 YggT family protein -
  PWH52_RS18335 yggU 3929095..3929385 (+) 291 WP_024555569.1 DUF167 family protein YggU -
  PWH52_RS18340 - 3929401..3929994 (+) 594 WP_024561875.1 XTP/dITP diphosphatase -
  PWH52_RS18345 hemW 3929987..3931123 (+) 1137 WP_024561874.1 radical SAM family heme chaperone HemW -
  PWH52_RS18350 - 3931194..3931520 (+) 327 Protein_3570 DUF559 domain-containing protein -

Sequence

Protein


Download         Length: 337 a.a.        Molecular weight: 36483.72 Da        Isoelectric Point: 6.8235

>NTDB_id=797618 PWH52_RS18320 WP_338463945.1 3926783..3927796(-) (pilT) [Franconibacter daqui strain HNU6-13]
MDMDELVALSVKHNVSDLHLCSALPARWRRRGRLEPVPHPLPEPETLLGEWLNETQRSELAFNGQLDFAVSLPGGARLRV
NAFRQQNGLSLALRLLPTRCPQLSSLDPPAALTGLLQAPDGLILVTGATGSGKSTTLAAMVDYLNQTLDGHILTLEDPVE
FQHASARCLIQQREVGIHCRSFSDGLRGALREDPDVILLGELRDSETIRLALSAAETGHLVLATLHTRGAAQAVERLVDV
FPAEEKIWVRSQLAGSLRAVLAQKLAESSDGGRVALYELLINTTAVANLIREGKSHQLPGQLETGRQQGMQSFAHSLASR
ICEGKIAAARPQAAKWG

Nucleotide


Download         Length: 1014 bp        

>NTDB_id=797618 PWH52_RS18320 WP_338463945.1 3926783..3927796(-) (pilT) [Franconibacter daqui strain HNU6-13]
ATGGATATGGATGAACTGGTGGCGCTTAGTGTAAAGCATAACGTTTCGGATCTACACCTGTGCAGCGCGTTGCCTGCGCG
ATGGCGTCGGCGCGGCAGGCTGGAGCCGGTGCCGCACCCGCTGCCTGAACCTGAAACCTTGCTCGGCGAGTGGCTTAATG
AAACCCAGCGCAGCGAGCTTGCCTTTAACGGGCAACTTGATTTCGCCGTCTCGCTGCCTGGCGGGGCGCGGCTGCGCGTC
AACGCCTTCCGGCAGCAAAACGGGCTTTCGCTCGCGCTGCGTTTGCTGCCGACGCGCTGTCCGCAGCTGTCGTCGCTCGA
TCCGCCAGCGGCGTTAACCGGGCTGCTGCAGGCGCCGGATGGCCTGATTCTGGTGACCGGGGCGACGGGCAGCGGAAAAT
CGACCACGCTGGCGGCGATGGTCGACTACCTCAACCAGACGCTGGATGGACATATCCTTACGCTGGAAGATCCGGTGGAG
TTTCAGCACGCCAGCGCACGTTGCCTGATTCAACAACGGGAGGTAGGCATTCATTGCCGAAGCTTCAGCGACGGTTTACG
CGGCGCGCTGCGGGAAGATCCGGATGTGATTTTACTGGGCGAACTGCGGGACAGCGAAACTATCCGGCTCGCCCTGAGCG
CGGCTGAAACGGGCCATCTGGTGCTGGCGACGCTGCACACGCGCGGCGCGGCGCAGGCGGTGGAGCGGCTGGTGGATGTC
TTCCCGGCGGAGGAGAAAATCTGGGTGCGCAGTCAACTTGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAACTGGCGGA
GTCGAGCGATGGCGGGCGGGTGGCGCTGTATGAGCTGCTTATCAACACCACGGCGGTGGCGAACCTTATCCGCGAAGGCA
AAAGCCATCAGTTGCCCGGCCAACTGGAAACCGGAAGACAGCAGGGGATGCAGAGCTTCGCGCACAGCCTGGCTTCGCGC
ATCTGTGAAGGAAAAATCGCCGCCGCGCGGCCGCAGGCGGCGAAGTGGGGATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(58-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.405

99.703

0.493
  pilT Vibrio cholerae strain A1552

49.405

99.703

0.493
  pilT Neisseria meningitidis 8013

48.378

100

0.487
  pilT Neisseria gonorrhoeae MS11

48.378

100

0.487
  pilT Pseudomonas aeruginosa PAK

48.795

98.516

0.481
  pilT Pseudomonas stutzeri DSM 10701

48.788

97.923

0.478
  pilT Acinetobacter baumannii strain A118

46.45

100

0.466
  pilT Acinetobacter nosocomialis M2

46.45

100

0.466
  pilT Acinetobacter baumannii D1279779

46.45

100

0.466
  pilT Acinetobacter baylyi ADP1

46.809

97.626

0.457
  pilT Legionella pneumophila strain Lp02

46.364

97.923

0.454
  pilT Legionella pneumophila strain ERS1305867

46.364

97.923

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.41

95.549

0.424
  pilU Pseudomonas stutzeri DSM 10701

41.867

98.516

0.412
  pilU Vibrio cholerae strain A1552

38.485

97.923

0.377