Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PTI97_RS04370 Genome accession   NZ_CP118099
Coordinates   846578..847615 (+) Length   345 a.a.
NCBI ID   WP_034771165.1    Uniprot ID   -
Organism   Exiguobacterium marinum strain a-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 841578..852615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PTI97_RS04345 (PTI97_04345) - 842290..842853 (-) 564 WP_274357331.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PTI97_RS04350 (PTI97_04350) - 842801..843256 (-) 456 WP_026826499.1 type IV pilus modification PilV family protein -
  PTI97_RS04355 (PTI97_04355) - 843415..844626 (+) 1212 WP_274357332.1 G5 domain-containing protein -
  PTI97_RS04360 (PTI97_04360) - 844623..844913 (+) 291 WP_274357333.1 hypothetical protein -
  PTI97_RS04365 (PTI97_04365) - 844915..846585 (+) 1671 WP_274357334.1 GspE/PulE family protein -
  PTI97_RS04370 (PTI97_04370) pilT 846578..847615 (+) 1038 WP_034771165.1 type IV pilus twitching motility protein PilT Machinery gene
  PTI97_RS04375 (PTI97_04375) - 847622..848839 (+) 1218 WP_026826494.1 type II secretion system F family protein -
  PTI97_RS04380 (PTI97_04380) - 848982..849455 (+) 474 WP_274357335.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PTI97_RS04385 (PTI97_04385) - 849554..850306 (+) 753 WP_274357336.1 prepilin peptidase -
  PTI97_RS04390 (PTI97_04390) pilM 850319..851884 (+) 1566 WP_274357337.1 pilus assembly protein PilM -
  PTI97_RS04395 (PTI97_04395) - 851881..852588 (+) 708 WP_026826490.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38439.30 Da        Isoelectric Point: 6.4220

>NTDB_id=790136 PTI97_RS04370 WP_034771165.1 846578..847615(+) (pilT) [Exiguobacterium marinum strain a-1]
MINQSIEVILTNAVEKRASDIHLTAGSPPIIRVDGSLITMGEEKVLPHHLEGYLKEIMTEDMRERLERDRDLDFSFGVPR
VSRFRVNAYYQRGTIAIAFRSISGEIPTLNELNLPTVLKRLIEKPHGLILVTGPTGSGKSTTLAAMIHEINRTQRKRIIT
LEDPIEYLHSHDQSIVDQREIGSDVMKFVSGLRAALRQDPDVILLGEMRDLETISTAMTAAETGHLVMATLHTSSAMSTV
SRIIDAFPAEQQDQIRTQLANVLLGVVSQRLFPRIDVPKGRVAAMEIMLGNPAVANLIRNEKEFQLPTVIQTSRQNGMQS
MDAAIEQLVRQGIINPNAVPTEEMR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=790136 PTI97_RS04370 WP_034771165.1 846578..847615(+) (pilT) [Exiguobacterium marinum strain a-1]
ATGATTAATCAATCGATTGAAGTCATCTTGACGAATGCGGTCGAGAAACGTGCCTCAGACATTCATCTGACAGCAGGCTC
ACCTCCTATCATTCGAGTAGATGGGAGTCTCATCACGATGGGTGAGGAGAAGGTATTACCTCATCATCTCGAAGGATATT
TAAAAGAAATTATGACGGAGGACATGCGGGAACGTTTAGAGCGTGACCGGGATCTTGACTTCTCGTTCGGTGTTCCACGC
GTTTCGCGTTTTCGAGTCAATGCTTACTATCAACGGGGTACGATTGCCATCGCATTCCGTTCCATTTCCGGGGAAATCCC
GACATTGAATGAGCTGAACCTTCCGACGGTGTTGAAGCGCTTGATTGAAAAGCCGCACGGTCTCATCCTTGTGACGGGTC
CGACCGGTTCAGGAAAATCAACGACGCTTGCGGCGATGATTCATGAGATCAATCGGACGCAGCGAAAACGGATCATCACG
TTAGAAGATCCAATCGAATATTTGCATTCCCATGACCAAAGCATCGTCGACCAACGGGAAATCGGCTCCGATGTCATGAA
GTTCGTGAGCGGGTTGCGGGCTGCTTTACGTCAAGATCCGGACGTCATTTTGCTCGGGGAGATGCGTGACTTAGAGACGA
TCTCGACGGCGATGACGGCGGCAGAGACGGGTCACCTCGTCATGGCGACGCTCCATACATCGAGTGCGATGTCGACCGTG
AGCCGAATCATCGATGCGTTCCCAGCTGAGCAACAAGACCAAATCCGGACGCAACTCGCTAACGTCCTTCTCGGGGTCGT
CAGTCAGCGGTTATTTCCTCGGATCGATGTGCCTAAAGGTCGTGTCGCCGCCATGGAAATCATGTTAGGGAATCCGGCTG
TCGCCAATTTGATTCGAAATGAAAAAGAATTCCAATTGCCGACTGTCATCCAGACGAGTCGCCAAAACGGGATGCAATCG
ATGGATGCTGCCATCGAGCAATTGGTACGGCAAGGAATCATCAATCCAAATGCCGTCCCGACAGAAGAGATGAGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.064

95.362

0.487
  pilT Acinetobacter baylyi ADP1

48.318

94.783

0.458
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

46.707

96.812

0.452
  pilT Vibrio cholerae strain A1552

46.707

96.812

0.452
  pilT Pseudomonas aeruginosa PAK

47.706

94.783

0.452
  pilT Acinetobacter baumannii strain A118

46.547

96.522

0.449
  pilT Acinetobacter nosocomialis M2

46.547

96.522

0.449
  pilT Acinetobacter baumannii D1279779

46.547

96.522

0.449
  pilT Neisseria meningitidis 8013

47.112

95.362

0.449
  pilT Neisseria gonorrhoeae MS11

46.364

95.652

0.443
  pilT Legionella pneumophila strain Lp02

46.084

96.232

0.443
  pilT Legionella pneumophila strain ERS1305867

46.084

96.232

0.443
  pilT Pseudomonas stutzeri DSM 10701

45.758

95.652

0.438
  pilU Vibrio cholerae strain A1552

41.159

95.072

0.391
  pilU Pseudomonas stutzeri DSM 10701

40.729

95.362

0.388