Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PQG02_RS01620 Genome accession   NZ_CP117768
Coordinates   247588..248712 (+) Length   374 a.a.
NCBI ID   WP_273766376.1    Uniprot ID   -
Organism   Nostoc sp. UHCC 0926     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 242588..253712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PQG02_RS01610 (PQG02_01655) grpE 243832..244581 (-) 750 WP_273766373.1 nucleotide exchange factor GrpE -
  PQG02_RS01615 (PQG02_01660) pilB/pilB1 245396..247408 (+) 2013 WP_273766374.1 type II/IV secretion system protein Machinery gene
  PQG02_RS01620 (PQG02_01665) pilT 247588..248712 (+) 1125 WP_273766376.1 type IV pilus twitching motility protein PilT Machinery gene
  PQG02_RS01625 (PQG02_01670) - 248788..250014 (+) 1227 WP_273766379.1 type II secretion system F family protein -
  PQG02_RS01630 (PQG02_01675) - 250157..251212 (-) 1056 Protein_215 IS630 family transposase -
  PQG02_RS01635 (PQG02_01680) - 251303..251863 (+) 561 WP_273766380.1 hypothetical protein -
  PQG02_RS01640 (PQG02_01685) - 252025..253047 (-) 1023 WP_273766382.1 saccharopine dehydrogenase-like oxidoreductase -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40937.26 Da        Isoelectric Point: 7.4489

>NTDB_id=787569 PQG02_RS01620 WP_273766376.1 247588..248712(+) (pilT) [Nostoc sp. UHCC 0926]
MEMMIEDLMEQLIEMGGSDLHLSAGLPPYFRISGKLTPIGEHVLTADQCQRLIFSMLNNTQRKTLEQTWELDCSYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEKLGLPDIVREMCDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHERQTQVRVQLSNSLVGVFSQTLVSKKNPKPGEYGRVMAQEILIVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADFYKAGTISFEAAMSKTSKPDEIQRLIGTATPQAAGAKPGAAARAH

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=787569 PQG02_RS01620 WP_273766376.1 247588..248712(+) (pilT) [Nostoc sp. UHCC 0926]
ATGGAAATGATGATTGAAGACCTGATGGAGCAGTTGATTGAAATGGGTGGCTCAGATTTGCATTTATCCGCAGGTTTGCC
TCCCTACTTCCGCATCAGTGGCAAACTCACCCCCATAGGTGAGCATGTATTAACAGCTGACCAATGTCAAAGGCTGATTT
TTAGTATGCTCAACAACACCCAACGTAAAACCTTGGAGCAGACCTGGGAGTTGGATTGTTCGTATGGTGTTAAGGGTTTA
GCTCGCTTCCGGGTCAATGTTTATAAAGAACGTGGTGCTTATGCTGCTTGCTTACGGGCATTAAGTTCTAAAATTCCTAA
CTTTGAAAAATTAGGTCTGCCAGATATTGTACGGGAAATGTGCGATAAGCCCAGAGGACTAATTCTAGTGACAGGCCCTA
CAGGTTCTGGCAAGACAACTACCCTAGCGGCAATGATCGATTTGATTAACCGCACCAAGGCGGAGCATATTTTAACGGTG
GAAGACCCAATTGAATTTGTCTACGAACCAATTAAAAGCTTGGTTCACCAGCGACAACTGGGTGAAGATACCAAGAGCTT
TGCTAATGCTTTGAAAGCAGCCCTGCGGGAAGATCCAGATATTATTCTGGTGGGAGAAATGCGCGATTTGGAAACGATTT
CTTTGGCGATTTCCGCAGCAGAAACAGGACACTTGGTATTTGGCACGCTACACACCAGTTCTGCTGCACAGACAGTTGAC
CGGATTATCGACGTTTTCCCCCATGAAAGACAAACTCAAGTGCGGGTGCAATTGTCTAACTCGTTAGTGGGGGTATTTAG
CCAAACCTTGGTGTCCAAGAAAAACCCTAAACCCGGTGAGTATGGTCGGGTGATGGCTCAAGAAATTCTGATTGTTACTC
CCGCTATTTCCAACTTGATTCGAGAAGGCAAAACATCTCAAATTTACTCAGCTATTCAAACTGGCGGCAAATTGGGGATG
CAAACTCTGGAGAAGGTTCTAGCTGATTTTTACAAAGCAGGAACGATCTCTTTTGAAGCTGCGATGTCTAAGACTTCTAA
GCCAGATGAAATCCAACGTCTCATCGGTACTGCTACACCGCAGGCAGCAGGTGCAAAACCCGGTGCGGCTGCTAGAGCGC
ATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.728

93.316

0.511
  pilT Pseudomonas stutzeri DSM 10701

53.868

93.316

0.503
  pilT Acinetobacter baylyi ADP1

53.314

92.781

0.495
  pilT Legionella pneumophila strain Lp02

53.026

92.781

0.492
  pilT Legionella pneumophila strain ERS1305867

53.026

92.781

0.492
  pilT Acinetobacter baumannii strain A118

52.45

92.781

0.487
  pilT Acinetobacter nosocomialis M2

52.45

92.781

0.487
  pilT Acinetobacter baumannii D1279779

52.45

92.781

0.487
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.956

98.128

0.471
  pilT Neisseria gonorrhoeae MS11

50.737

90.642

0.46
  pilT Neisseria meningitidis 8013

50.442

90.642

0.457
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.708

91.444

0.455
  pilT Vibrio cholerae strain A1552

49.708

91.444

0.455
  pilU Acinetobacter baylyi ADP1

38.244

94.385

0.361